Blood samples were analyzed using a veterinary hematology analyzer ABC Vet (HORIBA®, UK). The following parameters were determined: counts of white blood cells, red blood cells, platelets, granulocytes, lymphocytes, and monocytes, hematocrit, mean corpuscular volume, hemoglobin concentration, and red cell distribution width.
Abc vet
Manufactured by Horiba
Sourced in France, United Kingdom, Japan, Germany
The ABC Vet is a compact and versatile lab equipment designed for veterinary applications. It provides quick and accurate analysis of various biological samples. The core function of the ABC Vet is to perform automated hematology tests, delivering reliable results to support veterinary diagnosis and patient care.
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28 protocols using abc vet
1
Hematological Analysis of Blood Samples
2
Comprehensive Blood and Serum Analysis
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The blood collected from facial vein was analyzed for hematological parameters: counts of white blood cells, red blood cells, platelets, granulocytes, lymphocytes, monocytes, hematocrit, mean corpuscular volume, hemoglobin concentration , and red cell distribution width using a fully automated veterinary analyzer ABC Vet (HORIBA®, UK). The samples of sera separated from blood (collected by cardiac puncture) were analyzed using Spotchem EZ Chemistry Analyzer (Woodley; Lancashire, UK) and multiparameter strips: Spotchem II Panel V, according to the manufacturer’s instructions. The following biochemical parameters were measured: activities of ALP and alanine transaminase (ALT), concentrations of total protein (T-Pro), creatinine (Cre) and blood urea nitrogen (BUN).
3
Equine Blood Sampling for Injury Analysis
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Blood was collected from injured horses on the 1st (16 horses) or 3rd (12 horses) day after the injury depending when the injury was notified by an owner. Exact time of blood collection is presented in Table 1 . Control horses were blood-sampled on both 1st and 3rd day after the race. All blood samples were acquired by a jugular venipuncture into 20 ml syringes and transferred immediately into K2-EDTA tubes for hematological tests and plain tubes for SAA analysis. EDTA blood samples were kept in +4°C and examined within 5 h for routine hematological parameters in an automated analyzer (ABC Vet, Horiba ABX). Plain tubes were centrifuged at 4380g for 5 minutes, collected serum was frozen and stored at -20°C for SAA analysis. SAA concentrations were measured using an enzyme linked immunosorbent assay (PHASE SAA Assay, Tridelta Ltd) previously validated for use in equine studies [13 (link)–17 ]. Basic dilution of the samples was 1:1000. Samples with SAA values above the detection limit were further analyzed at the dilution of 1:2000.
4
Whole Blood Differential Analysis
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In Experiment B, on day 30, a whole blood differential count was performed (ABC Vet, Horiba ABX, France) using EDTA-treated blood, yielding counts of leukocytes, red blood cells, and platelets, and concentrations of hemoglobin, mean corpuscular hemoglobin, and mean hematocrit percentages and mean corpuscular volume.
5
Comprehensive Hematological Analysis
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The analysis of Red Blood Cell (RBC) included erythrocyte counts, hemoglobin concentration, hematocrit, Mean Corpuscular Volume (MCV), Mean Corpuscular Hemoglobin Concentration (MCHC). The White Blood Cell (WBC) included total and differential leukocyte counts. All hematological measurements were carried out in a hematology analyzer (ABC VET - Horiba® ABX Diagnostics). The differential leukocyte count was performed by optical microscopy (Nikon Eclipse E200®), 1000× magnification, of blood smears stained with Fast Panoptic kit (Laborclin®, São José do Rio Preto - SP).
6
Leukocyte Quantification in Mice
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Whole blood of each mouse was used for the quantitative determination of white blood cells. Differential leukocyte counts consisted of an evaluation of the total quantity for each type of leukocyte. The counting of total number of leukocytes (mm3) was performed on automatic blood cell analyzer ABC Vet (HORIBA®, United Kingdom). For mice, one blood drop was obtained by cardiac puncture and was immediately transferred to a microscope slide for a blood smear. Slides were stained using the Wright stain method (Lillie, 1972 ). Cell count was made using an optical microscope with a 100x magnification. For each slide, 100 cells were counted based in morphological criteria.
7
Anticoagulant Effects on Blood Cells in Rats
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The blood samples were collected from the tail arteries of 32 male Wistar rats (177.7 ± 13.5 g), at seven weeks old, after being anesthetized by an intraperitoneal injection of pentobarbital (45 mg/kg), using K2EDTA as an anticoagulant. The blood cells were counted 15, 30, and 60 min following injection into the right femoral vein of the vehicle (phosphate buffered saline, 1 mL/kg), UFH (150 U/kg, 1 mL/kg), PS (1.5 mg/kg, 1 mL/kg), or both (Figure 9 a), using the Animal Blood Counter (ABC Vet, Horiba ABX Sp. z o.o., Warsaw, Poland). The blood samples were collected from the heart and drawn into 3.13% trisodium citrate in a volume ratio of 9:1 at the end of the experiment. The platelet aggregation was measured according to the methods previously described, with the collagen addition at a concentration of 7.5 µg/mL.
8
Blood Collection and Analysis Protocol
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Blood was collected from anaesthetized animals (pentobarbital, 140 mg/kg, i.p.) via the right ventricle to a syringe with nadroparine (end concentration: 10 U/ml) for analysis of blood count and HbA1c, and the rest of the sample were centrifuged to obtain plasma (1000g, 5 min, 4°C). Complete blood count was analyzed within 15 minutes after collection (by automatic blood counter ABC Vet, HORIBA). HbA1c and total hemoglobin concentrations were measured using a biochemical analyser (ABX Pentra 400, HORIBA), and the ratio was given as a percentage of HbA1c. Glucose, aspartate aminotransferase, alanine aminotransferase, creatinine, albumin, and total protein were measured using colorimetric methods (ABX Pentra 400, HORIBA).
9
Comprehensive Euthanasia Blood Analysis
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On the day of euthanasia, blood
was first taken from the facial vein for hematological analyses performed
with a fully automated veterinary analyzer ABC Vet (HORIBA, UK): counts
of white blood cells, red blood cells, platelets, granulocytes, lymphocytes
(LYM), and monocytes; hematocrit value, mean corpuscular volume; hemoglobin
concentration; and red blood cell distribution width. Next, blood
was drawn postmortem by cardiac puncture, allowed to clot, and centrifuged
(800g, 10 min, RT). Serum isolated by centrifugation
(800g, 10 min, RT) was used for biochemical analysis
(using a SPOTCHEM EZ Chemistry Analyzer—Woodley Equipment and
multiparameter strips SPOTCHEM II Panel V, according to the manufacturer’s
instructions) and cytokine profiling. The levels of IL-1α, IL-1β,
IL-6, IL-10, IL-12p70, IL-17α, IL-23, IL-27, MCP-1, IFN-β,
IFN-γ, TNF-α, and GM-CSF were examined using the LEGENDplex
Mouse Inflammation Panel (13-plex) immunoassay (BioLegend) and a BD
LSRFortessa flow cytometer, and analyzed using LEGENDplex software
(BioLegend).
was first taken from the facial vein for hematological analyses performed
with a fully automated veterinary analyzer ABC Vet (HORIBA, UK): counts
of white blood cells, red blood cells, platelets, granulocytes, lymphocytes
(LYM), and monocytes; hematocrit value, mean corpuscular volume; hemoglobin
concentration; and red blood cell distribution width. Next, blood
was drawn postmortem by cardiac puncture, allowed to clot, and centrifuged
(800g, 10 min, RT). Serum isolated by centrifugation
(800g, 10 min, RT) was used for biochemical analysis
(using a SPOTCHEM EZ Chemistry Analyzer—Woodley Equipment and
multiparameter strips SPOTCHEM II Panel V, according to the manufacturer’s
instructions) and cytokine profiling. The levels of IL-1α, IL-1β,
IL-6, IL-10, IL-12p70, IL-17α, IL-23, IL-27, MCP-1, IFN-β,
IFN-γ, TNF-α, and GM-CSF were examined using the LEGENDplex
Mouse Inflammation Panel (13-plex) immunoassay (BioLegend) and a BD
LSRFortessa flow cytometer, and analyzed using LEGENDplex software
(BioLegend).
10
Intracardiac Blood Cell Analysis
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Blood samples were collected by an intracardiac route. The total number of cells was determined using an automated hematology counter (ABC Vet—Horiba ABX), whereas blood smears were stained with Rosenfeld solution to determine the differential count. A total of 100 cells were counted using a conventional optical microscope (Leica Microsystems, Wetzlar, Germany).
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