Digital mouse stereotaxic instrument

Orthotopic brain xenografting was generated as previously described.⁷, ⁸ Briefly, 6‐week‐old female BALB/c nude mice were divided randomly into the corresponding group, and each group had three mice. Animals were placed in a stereotaxic instrument (Stoelting Digital Mouse Stereotaxic Instrument), and their skull was exposed. The microsyringe (10 μl) was positioned with a stereotaxic instrument. Tumour cells exposed to different treatments were injected into the mouse brains by using a micropipette mounted on a stereotaxic instrument. Animals were imaged using the IVIS Lumina III in vivo imaging system (PerkinElmer). For more detail, please refer to our previous publication.⁸, ¹², ¹³

The FOC was fix to a digital mouse stereotaxic instrument (Stoelting Co.). The bregma was calibrated to be coordinate (0,0), and the FOC was scanned through the mouse motor cortex with 1 mm interval laterally and posteriorly to the bregma. EMG was performed using needle electrode inserted subcutaneously and parallel to the forelimb triceps brachii muscle. Reference electrode was inserted in the footpad. A ground electrode was inserted subcutaneously on the trunk and ipsilateral to the stimulation site. EMG signals were acquired using a Multi Clamp 700B amplifier (Molecular Devices), filtered at 1 to 5000 Hz, and digitized with an Axon DigiData 1550 digitizer (Molecular Devices).