Zetasizer nano zs series zen 3600

The Zetasizer Nano ZS series ZEN 3600 (Malvern Panalytical, Malvern, UK) was used to determine the zeta potential, measured by laser Doppler micro-electrophoresis (Smoluchowski’s theory). Fractions 1 and 2 from all three donors were diluted 1:50 in PBS, for a final volume of 1 mL. Each dilution was placed into a plastic, disposable folded capillary cell, and any air bubbles were removed from the cuvette through gentle tapping prior to recording. Three sets of 20 measurements were taken for each sample.

Liposomes were characterized for diameter, polydispersity index, conductivity, electrophoretic mobility, and charge (ζ potential) using a Zetasizer Nano ZS series ZEN3600 fitted with a 633 nm laser (Malvern Instruments Ltd.). Liposomes (50 μl) containing 15-HETE-PE, generated as above, were added to 950 μl deionized water and placed in a clear plastic ζ cell (DTS 1070, Malvern) and equilibrated for 2 minutes at 25°C prior to measurement. Each was measured at least 4 times. Liposome diameter was also measured using by nanoparticle tracking analysis using a NanoSight LM10 system (Malvern Instruments Ltd.) running NTA-software v2.3 and configured with a temperature controlled, 488nm LM14 laser module and a high-sensitivity camera system (OrcaFlash2.8, Hamamatsu C11440). In brief, 4-mM liposomes were diluted 1,000 times in nano-particle free water (Fresenius Kabi) and analyzed under controlled fluid flow at 37°C, as previously described (49 (link)). Data were combined from 4 preparations done on different days, with each analyzed 5 times.