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E el r0015c

Manufactured by Elabscience
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Sourced in China

E-EL-R0015c is a lab equipment product from Elabscience. It is a centrifuge that is used to separate different components of a liquid sample by applying centrifugal force.

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Lab products found in correlation

E el r0012c, by Elabscience (13 mentions) E el r2856c, by Elabscience (11 mentions) E el r0019c, by Elabscience (5 mentions) E el r0567c, by Elabscience (3 mentions) Seg886hu, by Cloud-Clone (2 mentions) Seg886ra, by Cloud-Clone (2 mentions) E el h0109c, by Elabscience (2 mentions) E el r0505c, by Elabscience (2 mentions) E el r0016c, by Elabscience (2 mentions) Trizol, by Thermo Fisher Scientific (2 mentions) Occludin 27260 1 ap, by Proteintech (1 mentions) Tnf α ab6671, by Abcam (1 mentions) β actin gb11001, by Wuhan Servicebio Technology (1 mentions) Sod assay kit, by Nanjing Jiancheng (1 mentions) Multiskan mk3, by Thermo Fisher Scientific (1 mentions) E el h0149c, by Elabscience (1 mentions) E el h0102c, by Elabscience (1 mentions) E el r0009c, by Elabscience (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Lipopolysaccharide, by Merck Group (1 mentions)

19 protocols using e el r0015c

1

ELISA for Rat IL-6 and TNF-α

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IL-6 and TNF-α levels in the serum of rats and cells were measured using a rat IL-6 ELISA kit (E-EL-R0015c; Elabscience, Wuhan, China) and a rat TNF-α ELISA kit (E-EL-R2856c; Elabscience) respectively according to the manufacturer’s instructions.
Ye S., Qi X., Liu Y., Zhuang L, & Gu Z. (2023). RIP1/3-dependent programmed necrosis induces intestinal injury in septic rats: RIP1/3-dependent programmed necrosis induces intestinal injury. Acta Biochimica et Biophysica Sinica, 56(1), 106-113.
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2

High-Fat Diet Induced Gut Barrier Dysfunction

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The high-fat diet (HFD, D12492) chow was purchased from Beijing Huafukang Bioscience; standard rat chow (SRC) was offered by the experimental animal center of Wuhan University Renmin Hospital. Rat ELISA kits of interleukin- (IL-) 1β (E-EL-R0012c) and IL-6 (E-EL-R0015c) were purchased from Elabscience (Wuhan, China). Diamine oxidase (DAO) assay kit (A088-1) and endotoxin assay kit (E039-1-1), reduced form of glutathione (GSH) assay kit (A006-2-1) and maleic dialdehyde (MDA) assay kit (A003-1-2), and superoxide dismutase (SOD) assay kit (A001-2-2) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Zonula occluden-1 (Zo-1) (21773-1-AP) and occludin (27260-1-AP) antibodies were purchased from Proteintech (Wuhan, China); RIP3 (ab56164) and TNF-α (ab6671) antibodies were purchased from Abcam (Cambridge, UK); TLR4 (GB11519), NF-κB P65 (GB11142), and β-actin (GB11001) antibodies were purchased from Servicebio (Wuhan, China). TAK242 (HY-11109), an inhibitor of TLR4, was purchased from MCE (Shanghai, China).
Su Y.R., Hong Y.P., Mei F.C., Wang C.Y., Li M., Zhou Y., Zhao K.L., Yu J, & Wang W.X. (2019). High-Fat Diet Aggravates the Intestinal Barrier Injury via TLR4-RIP3 Pathway in a Rat Model of Severe Acute Pancreatitis. Mediators of Inflammation, 2019, 2512687.
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3

Quantification of Hippocampal IL-1β and IL-6

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Rats were killed with overdose pentobarbital sodium after the 7-day house and hippocampus tissues were collected. The production of IL-1β (E-EL-R0012c, Elabscience Biotechnology, Wuhan, China) and IL-6 (E-EL-R0015c, Elabscience Biotechnology, Wuhan, China) in hippocampus tissues was detected using corresponding ELISA kits according to the manufacturer’s instructions: briefly, hippocampus tissues were homogenized and then 100-μl sample was incubated in one well of ELISA plate at 37°C for 90 min Afterward, 100 μl biotinylation antibody was added into the well and incubated at 37°C for 60 min. Then 100 μl enzyme-binding solution was added into the mixture and incubated at 37°C for 30 min. Finally, 50 μl TMB substrate was added and incubated at 37°C for 30 min. The OD values at 450 nm of different samples were detected using a Microplate Reader (MULTISKAN MK3, Thermo).
Zheng B., Zhang S., Ying Y., Guo X., Li H., Xu L, & Ruan X. (2018). Administration of Dexmedetomidine inhibited NLRP3 inflammasome and microglial cell activities in hippocampus of traumatic brain injury rats. Bioscience Reports, 38(5), BSR20180892.
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4

Quantifying CIRP and Inflammatory Factors

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The CIRP concentrations in the human and rat serum were detected using CIRP ELISA kits (SEG886Hu and SEG886Ra, Cloud-Clone Corp.) according to the manufacturer's protocol. The concentration difference of CIRP in the human serum between the two time points was recorded as ΔCIRP. The ELISA kits for interleukin 6 (IL-6, E-EL-H0102c and E-EL-R0015c, Elabscience), IL-1β (E-EL-H0149c and E-EL-R0012c, Elabscience), and tumor necrosis factor alpha (TNF-α, E-EL-H0109c and E-EL-R2856c, Elabscience) were used to detect inflammatory factors in the rat serum and the cell culture medium.
Zheng X., Fan Y., Li J., Ma T., Li Y., Wang Q., Yan Y, & Liu W. (2022). Change in Oxidative Stress and Mitochondrial Dynamics in Response to Elevated Cold-Inducible RNA-Binding Protein in Cardiac Surgery-Associated Acute Kidney Injury. Oxidative Medicine and Cellular Longevity, 2022, 3576892.
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5

Measurement of Inflammatory Cytokines in Rat Plasma

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The level of IL-1, IL-6, IFN, and TNF-α in the plasma of rats was measured by ELISAs. IL-1 (E-EL-R0012c), IL-6 (E-EL-R0015c), IFN (E-EL-R0009c), and TNF-α (E-EL-R0019c) ELISA kits were purchased from Elabscience (Wuhan, China). The procedures were conducted according to the instruction manuals for the kits. Sterile PBS was used as the control.
Li Y., Wang Y., Chen Y., Wang Y., Zhang S., Liu P., Chen Z., Song P., Luo L., Luo Y., Dang Y, & Zhao L. (2020). Corilagin Ameliorates Atherosclerosis in Peripheral Artery Disease via the Toll-Like Receptor-4 Signaling Pathway in vitro and in vivo. Frontiers in Immunology, 11, 1611.
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6

Anti-inflammatory Pathway Regulation

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SB203580 (Cat. HY-10256) was purchased from MedChemExpress (Monmouth, NJ, America). Lipopolysaccharide was purchased from Sigma (Cat. L4130, St. Louis, MO, America). Antibodies for MAPK14 (Cat. 8690S) and β-actin (Cat. 4970S) were purchased from Cell Signaling Technology (Danvers, Massachusetts, America). Cell counting kit-8 (Cat. C0038) was purchased from Beyotime Biotechnology (Shanghai, China). Enzyme-linked immunosorbent assay (ELISA) detection kit of pro-inflammatory cytokines TNF-α (E-EL-R2856c), interleukin (IL)-6 (E-EL-R0015c), and IL-1β (E-EL-R0012c) were purchased from Elabscience (Wuhan, China). In situ cell death detection kit (Cat. 11684795910) was purchased from Roche (Huntsville, German).
She H., Tan L., Wang Y., Du Y., Zhou Y., Zhang J., Du Y., Guo N., Wu Z., Li Q., Bao D., Mao Q., Hu Y., Liu L, & Li T. (2023). Integrative single-cell RNA sequencing and metabolomics decipher the imbalanced lipid-metabolism in maladaptive immune responses during sepsis. Frontiers in Immunology, 14, 1181697.
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7

Multiplex Protein Detection in Biological Fluids

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Cold‐inducible RNA‐binding protein (SEG886Hu and SEG886Ra, Cloud‐Clone Corp.), mitochondrial transcription factor A (TFAM; SEH050Hu, Cloud‐Clone Corp.), interleukin 6 (IL‐6; E‐EL‐H0109c and E‐EL‐R0015c, Elabscience), interleukin 1 beta (IL‐1β; E‐EL‐R0012c, Elabscience), tumour necrosis factor‐alpha (TNF‐α; E‐EL‐R2856c, Elabscience) and high mobility group protein B1 (HMGB1; E‐EL‐R0505c, Elabscience) ELISA kits were used to detect these molecules in the UW solution, human serum and rat serum.
Liu W., Fan Y., Ding H., Han D., Yan Y., Wu R., Lv Y, & Zheng X. (2021). Normothermic machine perfusion attenuates hepatic ischaemia‐reperfusion injury by inhibiting CIRP‐mediated oxidative stress and mitochondrial fission. Journal of Cellular and Molecular Medicine, 25(24), 11310-11321.
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8

Comprehensive Serum Biomarker Profiling

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Enzyme-linked immunosorbent assay (ELISA) kits were used to measure the serum concentrations of HSP70 (DYC1663E, Minneapolis, MN, USA), follicle stimulating hormone (FSH)(CSB-E06869r, Cusabio Biotech CO.,Ltd., Wuhan, China), luteinizing hormone (LH) (CSB-E12654r, Cusabio Biotech CO.,Ltd., Wuhan, China), estradiol (E2) (CSB-E05110r, Cusabio Biotech CO.,Ltd., Wuhan, China), progesterone (P) (CSB-E07282r, Cusabio Biotech CO.,Ltd., Wuhan, China), testosterone (T)(E-EL-0072c, Elabscience Biotechnology Co.,Ltd., Wuhan, China), insulin (CSB-E05070r, Elabscience Biotechnology Co.,Ltd., Wuhan, China), C-reactive protein (CRP) (E-EL-R0022c, Elabscience Biotechnology Co.,Ltd., Wuhan, China), interleukin (IL)-6(E-EL-R0015c, Elabscience Biotechnology Co.,Ltd., Wuhan, China), IL-18(E-EL-R0567c, Elabscience Biotechnology Co.,Ltd., Wuhan, China), tumor necrosis factor (TNF)-α. (E-EL-R0019c, Elabscience Biotechnology Co.,Ltd., Wuhan, China). There were standard curves used to calculate for all these kits. The coefficients of variation within and between plates were less than 10%.
Wu G., Hu X., Ding J, & Yang J. (2019). Abnormal expression of HSP70 may contribute to PCOS pathology. Journal of Ovarian Research, 12, 74.
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9

Cytokine Profiling in Serum

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The levels of interleukin (IL)-6 (E-EL-R0015c; Elabscience, China), IL-1β (E-EL-R0012c; Elabscience), and IL-18 (E-EL-R0567c; Elabscience) in the serum were determined using ELISA kits and the 96-well plates placed into a multimode reader (SYNERGR LX; BioTEK).
Chen L., Lin B., Yang J., Zhong L., Xiong X, & Wang X. (2023). Hydrogen sulfide alleviates ischemia induced liver injury by repressing the SPHK1/S1P pathway. Annals of Translational Medicine, 11(2), 73.
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10

Blood Sampling and ELISA Analysis in Rat Model

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On the 3rd day, after HRV monitoring, 6 rats from each group were anesthetized with isoflurane inhalation [17 (link), 18 (link)]; then, the chest cavity was dissected, and 2 mL of blood was taken from the right atrium for ELISA and 2 mL for flow cytometry (FCM). For ELISA, blood was preserved at room temperature for 2 h prior to centrifugation at 3000 r/min at 4°C for 10 min; then, the upper layer of the serum was separated and stored in the refrigerator at -80°C for detection. For FCM, fresh blood was taken for testing. According to the manufacturer's instructions [19 (link)], the serum levels of TNF-α, IL-1α, IL-10, IL-6, HMGB1, and sCD14 were determined with ELISA.
The main related antibodies include: rat anti-TNF-α (lot number: E-EL-R0019c, manufacturer: Elabscience), rat anti-IL-1α (lot number: E-EL-R0011c, manufacturer: Elabscience), rat anti-IL-6 (lot number: E-EL-R0015c, manufacturer: Elabscience), rat anti-IL-10 (lot number: E-EL-R0016c, manufacturer: Elabscience), rat anti-HMGB-1 (lot number: E-EL-R0505c, manufacturer: Elabscience), and rat anti-sCD14 (lot number: CSB-E11178r, manufacturer: Cusabio).
Li H.B., Zhou Y., Zhao A.H, & Guo L.L. (2020). Exploring the Mechanism on the Medullary Visceral Zone Inhibiting the Cholinergic Anti-inflammatory Pathway Induced by Sepsis. Mediators of Inflammation, 2020, 1320278.
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