DU145 cells (1.0 × 105 cells) were plated into 35 mm high μ dishes (ibidi GmbH, Am Klopferspitz, Germany). The cells were washed once with PBS and treated with DMSO or acacetin (50 μM) for 24 h. After washing with PBS twice, the attached cells were fixed with 4% paraformaldehyde in PBS for 10 min at room temperature. The fixed cells were permeabilized with 0.2% Triton X-100 for 10 min and blocked with 1.0% BSA in PBS for 1 h. The cells were incubated with an anti-STAT3 antibody followed by goat-anti-rabbit IgG-FITC secondary antibody. The nuclei were counterstained with 2 μg/mL of DAPI in PBS for 2 min. All images were acquired on a laser scanning confocal microscope (LSM 510 META; Carl Zeiss, St. Cloud, MN, USA) and analyzed with the LSM Version 3.2 software (Carl Zeiss).
Lsm version 3
Manufactured by Zeiss
Sourced in United States
The LSM Version 3.2 software is a comprehensive imaging solution for microscopy applications. It provides a user-friendly interface for the acquisition, processing, and analysis of high-quality images. The software supports a wide range of Zeiss microscope models and offers advanced features for enhanced imaging capabilities.
Automatically generated - may contain errors
Lab products found in correlation
35 mm high μ dishes, by Ibidi (2 mentions)
Lsm 510 meta, by Zeiss (2 mentions)
4 6 diamidino 2 phenylindole (dapi), by Santa Cruz Biotechnology (1 mentions)
Goat anti rabbit igg fitc secondary antibody, by Santa Cruz Biotechnology (1 mentions)
Anti stat3 antibody, by Cell Signaling Technology (1 mentions)
2 protocols using lsm version 3
1
STAT3 Localization in DU145 Cells
2
STAT3 Activation in DU145 Cells
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DU145 cells (1.0 × 105 cells) were plated into 35‐mm high‐μ dishes (ibidi GmbH, Am Klopferspitz, Germany). The cells were washed once with PBS and treated with DMSO or HCA (20 μmol/L) for 1 or 24 hours. After washing with PBS twice, the attached cells were fixed with 4% paraformaldehyde in PBS for 10 minutes at room temperature. The fixed cells were permeabilized with .2% Triton X‐100 for 10 minutes and blocked with 1.0% BSA in PBS for 1 hour. The cells were incubated with an anti‐STAT3 antibody (Cell Signaling, Danvers, MA, USA) followed by goat anti‐rabbit IgG‐FITC secondary antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA). The nuclei were counterstained with 2 μg/mL DAPI (Santa Cruz Biotechnology) in PBS for 2 minutes. All images were acquired on a laser scanning confocal microscope (LSM 510 META; Carl Zeiss, St. Cloud, MN, USA) and analyzed with LSM Version 3.2 software (Carl Zeiss).
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