The reactants were grouped into four reaction mixtures: glucosamine-cyclohexylamine, glucosamine-benzylamine, glucose-cyclohexylamine, and glucose-benzylamine. In these experiments, the main factors for investigation were two folds of reaction times and initial reaction concentration ratios. The reaction times ranged from 0.5, 1.0, 1.5, 2.0, and 2.5 h. The ratio of the initial reactant concentrations ranged from 10:1, 5:1, 1:1, 1:5, and 1:10. The initial reaction pH was fixed at pH 9.0 using 0.1 M NaOH and 0.1 M HCl accordingly and verified by the use of a pH meter (Metrohm 827 pH lab, Metrohm AG, Herisau, Switzerland). The reactants were prepared at concentrations of 0.1 M by dissolving their respective quantities in distilled water. For each concentration ratio, the final concentration of the reactant with the smaller ratio was achieved by appropriate dilution using distilled water. Equivalent volumes of the reactants were then combined together in order to come up with the final reactants mixture to undergo the Maillard reaction. Aliquots of 5 mL of the reactants mixture at the different concentration ratios were prepared and kept in covered test tubes. The temperature was kept constant at 120°C in a dry oven (Dongwon Scientific System). The reactions were conducted in triplicate.
827 ph lab
Manufactured by Metrohm
Sourced in Switzerland, Germany, Japan
The 827 pH lab is a compact and versatile pH measurement device designed for laboratory use. It features a large, easy-to-read display and intuitive user interface. The 827 pH lab is capable of performing accurate pH measurements and can be used with a wide range of pH electrodes.
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Lab products found in correlation
Malvern zetasizer nano s, by Malvern Panalytical (2 mentions)
Nano z, by Malvern Panalytical (2 mentions)
Sodium sulphate na2so4, by Merck Group (1 mentions)
Citric acid, by Merck Group (1 mentions)
Uv 1800 uv vis spectrophotometer, by Shimadzu (1 mentions)
Type d8 advance, by Bruker (1 mentions)
Icap 6000 series spectrometer, by Thermo Fisher Scientific (1 mentions)
Advance 400 mhz spectrometer, by Bruker (1 mentions)
Zetasizer nano z, by Malvern Panalytical (1 mentions)
Atomic absorption spectrometer, by PerkinElmer (1 mentions)
Ultrospec 2000, by GE Healthcare (1 mentions)
Brookfield dv 3 ultra, by Ametek (1 mentions)
Biotrode, by Metrohm (1 mentions)
Lactic acid, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
Agilent 1200 series, by Agilent Technologies (1 mentions)
761 compact ic, by Metrohm (1 mentions)
34 protocols using 827 ph lab
1
Maillard Reaction Kinetics Optimization
2
Phycocyanin Stability in Aqueous Solutions
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Phycocyanin was supplied as the commercial product Linablue G1 from DIC Spirulina (Chuo-ku, Tokyo 103-8233, Japan). This formulation contained 40% Spirulina colour, including phycocyanin and other proteins, 5% tri-sodium citrate, and 55% D-trehalose.
Citric acid (Sigma-Aldrich) in demineralised water was used to prepare standard aqueous solutions with pH 6, 5, 4, 3, 2.5, 2, and 1.9, all ± 0.1. The final pH was measured using a Metrohm 827 pH lab. SDS, and sodium sulphate (Na2SO4) were from Sigma-Aldrich.
Citric acid (Sigma-Aldrich) in demineralised water was used to prepare standard aqueous solutions with pH 6, 5, 4, 3, 2.5, 2, and 1.9, all ± 0.1. The final pH was measured using a Metrohm 827 pH lab. SDS, and sodium sulphate (Na2SO4) were from Sigma-Aldrich.
3
pH-Dependent Solubility and Octanol-Water Partitioning of ABN401
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Solutions at pH values of 1 to 12 were prepared using HCl and NaOH. At first, 10−1 N, 10−2 N, 10−3 N, 10−4 N, 10−5 N, and 10−6 N of HCl and NaOH solutions were prepared and their pH values were measured with a pH meter (Metrohm 827 pH lab, Zurich, Switzerland). One milliliter of the 10−4 N, 10−5 N, and 10−6 N of HCl and NaOH solutions was withdrawn and diluted in a 100 mL flask for more coordination. In each prepared solution (n = 3), excess ABN401 was dissolved and mixed for 24 h. All samples were centrifuged for 1 min at 12,000 rpm. The pH solubility of ABN401 was analyzed by HPLC and the pKa was determined by plotting a graph of log(solubility) against pH.
ABN401 solution (10 μg/mL) was mixed with octanol at a ratio of 1:1 in a glass vial and placed horizontally in a Digital Bio Rotator (SeoLin Bioscience, Seongnam, Korea), and shaken at a constant 90 rpm for 24 h. After 6 h of equilibrium under room temperature, two phases of water and octanol were separated using a separating funnel. The experiment was repeated five times. The concentration of ABN401 at each phase was analyzed by HPLC and log P and log D were calculated using the Henderson-Hasselbalch equation below with the previously determined pKa value.
ABN401 solution (10 μg/mL) was mixed with octanol at a ratio of 1:1 in a glass vial and placed horizontally in a Digital Bio Rotator (SeoLin Bioscience, Seongnam, Korea), and shaken at a constant 90 rpm for 24 h. After 6 h of equilibrium under room temperature, two phases of water and octanol were separated using a separating funnel. The experiment was repeated five times. The concentration of ABN401 at each phase was analyzed by HPLC and log P and log D were calculated using the Henderson-Hasselbalch equation below with the previously determined pKa value.
4
Potentiometric Redox Potential Determination
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For each sample, the redox potential was determined using a potentiometric method that uses a pH meter (Metrohm 827 pH Lab) equipped with a platinum electrode (combined Pt-ring 6.0451.100). The potential was expressed in millivolts (mV).
5
Porcine Palatal Tissue Dissolution Assay
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A simple yet reliable weight loss assay 19 was used to test the soft tissue dissolution efficacy of test and control solutions. Twenty-four frozen specimens of similar volume and surface area were obtained from porcine palatal mucosa with a round punch (8 mm) applied at the crest of the rugae. Specimens were thawed at room temperature in a humid environment. They were blotted dry and then weighed using a precision balance (AT 261; Mettler Instrumente AG, N€ anikon-Uster, Switzerland). The specimens were then immersed without agitation in 10 mL test or control solutions for 20 minutes in cylindrical 40-mL polypropylene containers with a plastic lid (Semadeni, Ostermundigen, Switzerland). Subsequently, specimens were immersed in 0.1 mol/L sodium thiosulfate (Na 2 S 2 O 3 ) to stop the oxidizing action of H 2 O 2 or NaOCl, blotted dry, and weighed again. Tests were repeated 5 times (n 5 6) on different experimental days. In each run, 1 specimen per group was assessed to avoid bias.
The weight loss was calculated in the percent of the original tissue weight. The pH values of the test and control solutions before and after exposure to the soft tissue were determined using a calibrated pH electrode (827 pH lab, Deutsche Metrohm). These experiments were also performed at room temperature (25 C).
The weight loss was calculated in the percent of the original tissue weight. The pH values of the test and control solutions before and after exposure to the soft tissue were determined using a calibrated pH electrode (827 pH lab, Deutsche Metrohm). These experiments were also performed at room temperature (25 C).
6
Evaluating Sealers' pH Stability
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Polytetrafluoroethylene (PTFE) tubes (1.5 mm inner diameter, 10 mm length) were filled with sealers and left to completely set. The PTFE tubes were submerged in falcon tubes containing 10 ml of deionized water. The tubes were incubated at 37 °C and pH measurements were performed after 3 h, 6 h, 9 h, 24 h, 3 days, 7 days, 1 month, and 3 months (827 pH Lab, Deutsche Metrohm GmbH & Co. KG, Filderstradt, Germany).
7
Measuring Tea Infusion pH
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To exclude the possibility of the inhibition of tea film formation being caused by acidic conditions in the tea infusion, the pH was measured before and after the brew time as well as after rheological measurements. A pH meter (Metrohm 827 pH lab, Switzerland) was used for this purpose. Three brews were made and measured.
8
Glucose Modulation of S. aureus Growth and Impact on P. aeruginosa Viability
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ON cultures of S. aureus were diluted to an optical density at 600 nm (OD600) of 0.01 in TSB with increasing glucose concentrations (0 to 1%), shaking at 180 rpm at 37°C. Relative growth, determined by optical density at 600 nm, OD600 (UV-1800 UV-VIS spectrophotometer, Shimadzu, Japan), and pH (827 pH lab, Metrohm, CH) were determined after ON culturing.
A similar approach was conducted with variousS. aureus strains (see Table S1 in Supplemental File 1), where pH was measured in ON cultures grown with 1% glucose. In addition, supernatants were prepared from ON cultures, and P. aeruginosa was inoculated into supernatant with an optical density of 0.01 (OD600). Culture flasks were cultured ON at 37°C, shaking at 180 rpm. The viability of P. aeruginosa was assessed by plating aliquots from the ON cultures on LB agar plates (growth or no growth). Plates were incubated ON at 37°C.
A similar approach was conducted with various
9
Synthesis and Characterization of γ-MnO2
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The γ-MnO2 synthesis and electrochemical analytical characterizations were performed by methods such as X-ray diffraction (XRD, Bruker, Type D8- ADVANCE), electrochemical impedance spectroscopy (EIS, µ3AUT70980), cyclic voltammetry (CV, model-µ3AUT70980), field emission-scanning electron microscopy (FE-SEM, JSM-7610F Schottky Field Emission Scanning Electron Microscope, 25.0 kV, JOEL), battery tester (Hioki model: BT 3554), pH meter (Metrohm, 827 pH lab), Brunauer–Emmett–Teller (BET, Functional Capability, NOVA Company, Japan), and X-ray photoelectron spectroscopy (XPS, XPRT Kratos—AXIS Nova, Kratos Analytical Ltd., Shimadzu, Japan).
10
Analytical Determination of Nb and Ta
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Nb and Ta concentrations were determined by ICP-OES using an iCAP 6000 series spectrometer (Thermo Scientific). The samples were diluted in 2% HNO 3 containing 0.7% H 2 O 2 . Quantitative analyses of Nb and Ta were performed at the 309.418 nm and 240.063 nm spectral emission lines, respectively. Nb concentrations were also confirmed by UV-vis spectroscopy using a recently reported method. 10 All UV spectra were measured on a Cary 100-Scan spectrophotometer in double beam mode. pH measurements were performed with an 827 pH-lab (Metrohm) pH-meter and a low alkaline error combined electrode (Unitrode, Metrohm). The pH-meter was calibrated with standards at pH 4.01, 7.00 and 12.00 (Mettler Toledo). Water contents in the organic phase were determined using a Mettler Toledo V20 volumetric Karl-Fisher titration device filled with the Hydranal®-Composite 5 reagent. Water titrations were performed in anhydrous methanol. Chloride ions released in the aqueous phase by Aliquat® 336 were titrated by the classical argentometric method. Solution-state 23 Na NMR spectra were acquired on a Bruker Advance 400 MHz spectrometer. 23 Na NMR spectra were referenced to a 1.0 M NaCl aqueous solution.
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