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Hsv 1

Manufactured by Agilent Technologies
Sourced in Denmark

The HSV-1 is a laboratory equipment product designed for the detection and analysis of the Herpes Simplex Virus type 1 (HSV-1). The core function of this product is to facilitate the identification and quantification of HSV-1 in various sample types, such as clinical specimens or laboratory-cultured materials.

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3 protocols using hsv 1

1

Comprehensive Immunophenotyping Panel

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The following antibodies (Abs) were obtained from BD Biosciences (San Jose, CA), eBioscience (San Diego, CA), BioLegend (San Diego, CA), or Miltenyi Biotec, (Bergisch Gladbach, Germany) unless otherwise noted: fluorochrome-labeled Ab (clone) against CD45 (30-F11 and HI30), PDCA-1 (927 and JF05–1C2.4.1), CD45R/B220 (RA3–6B2), Siglec-H (eBio440c), CD11c (HL3), Ly6C (HK1.4), Ly49Q (clone number 2000000; MBL International Corporation, Woburn, MA), Gr-1 (RB6–8C5), Ly6G (1A8), CD11b (M1/70), CD68 (FA-11), CD4 (RM4–5), CD3 (17A2), CD19 (6D5), IFN-γ (XMG1.2), F4/80 (BM8), BDCA-2 (201A), BDCA-4 (12C2), β-III-Tubulin (TuJ-1; R&D Systems, Minneapolis, MN), and HSV-1 (polyclonal; Dako, Carpinteria, CA). Fluorochrome-conjugated rat IgG1, IgG2a, IgG2b, IgG2c, mouse IgG1, IgG2a, and Armenian hamster IgG1 were used as isotype-matched controls.
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2

Comprehensive Immunophenotyping Panel

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The following antibodies (Abs) were obtained from BD Biosciences (San Jose, CA), eBioscience (San Diego, CA), BioLegend (San Diego, CA), or Miltenyi Biotec, (Bergisch Gladbach, Germany) unless otherwise noted: fluorochrome-labeled Ab (clone) against CD45 (30-F11 and HI30), PDCA-1 (927 and JF05–1C2.4.1), CD45R/B220 (RA3–6B2), Siglec-H (eBio440c), CD11c (HL3), Ly6C (HK1.4), Ly49Q (clone number 2000000; MBL International Corporation, Woburn, MA), Gr-1 (RB6–8C5), Ly6G (1A8), CD11b (M1/70), CD68 (FA-11), CD4 (RM4–5), CD3 (17A2), CD19 (6D5), IFN-γ (XMG1.2), F4/80 (BM8), BDCA-2 (201A), BDCA-4 (12C2), β-III-Tubulin (TuJ-1; R&D Systems, Minneapolis, MN), and HSV-1 (polyclonal; Dako, Carpinteria, CA). Fluorochrome-conjugated rat IgG1, IgG2a, IgG2b, IgG2c, mouse IgG1, IgG2a, and Armenian hamster IgG1 were used as isotype-matched controls.
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3

Immunohistochemical Analysis of Tumor Samples

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Excised subcutaneous tumors and lymph nodes were formalin-fixed, paraffin-embedded, and cut into 4-μm sections using a microtome. Antibodies against HSV-1 (Dako, Glostrup, Denmark), enhanced green fluorescent protein (EGFP; Abcam, Cambridge, UK), anti-mouse Ki67 (Abcam), and mucin 1 (MUC 1; Abcam) were used for immunohistochemistry. EnVision+ System-HRP Labeled Polymer Anti-Rabbit IgG (Dako) was used as a secondary antibody. Immunostaining was visualized using DAB Substrate Kit (Vector Laboratories, Burlingame, CA, USA). Some sections were counterstained with H&E.
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