Hydrogenated soybean phospholipids (HSPC), cholesterol (Chol), and distearoyl phosphoethanolamine-PEG2000-Acrylamide (DSPE-PEG2000-ACA) were purchased from Shanghai Ponsure Biotech, Inc. Four-arm poly(ethylene glycol) amine (Tetra-PEG-amine) was provided by Xiamen Sinopeg company. Dibenzocyclooctyne-acid (DBCO acid, ≥95%) was supplied by Xi’an ruixi Biological Technology Co., Ltd. O-(7-azabenzotriazol-1-yl)-N,N,N’,N’-tetramethyluronium hexafluorophosphate (HATU, 99%) and glutathione (GSH, 99%) were purchased from J&K Scientific Ltd. N,N-diisopropylethylamine (≥99%, DIEA), N,N’-methylenebisAcrylamide (99%, MBAA), N,N,N’,N’-tetramethylethylenediamine (99%, TEMED), and anhydrous N,N-dimethylformamide (99.8%, DMF) were provided by Sigma. Ammonium persulfate (99.99%, APS) and 1,11-diazido-3,6,9-trioxaundecane (≥95%, N3-PEG3-N3) were supplied by Aladdin. Acrylamide (99%, AM) was purchased from Adamas. Ethyl ether (≥99.7%, Et2O) and chloroform (≥99%, CHCl3) were provided by Sinopharm chemical reagent Co., Ltd. Ammonium sulfate (≥99%, (NH4)2SO4) and PBS buffer (10×, pH 7.4) were supplied by Sangon Biotech company. The PBS buffer used in all experiments was diluted ten times. Deionized water was used to prepare all aqueous solutions. Other reagents and solvents were used as received.
Ethyl ether
Manufactured by Sinopharm
Sourced in China
Ethyl ether is a colorless, highly volatile, and flammable liquid. It has a distinctive sweet odor. The primary function of ethyl ether is as a solvent in chemical and pharmaceutical applications.
Automatically generated - may contain errors
Lab products found in correlation
Acetone, by Sinopharm (4 mentions)
Chloroform, by Sinopharm (3 mentions)
Sulfuric acid, by Sinopharm (3 mentions)
Hydrochloric acid (hcl), by Sinopharm (3 mentions)
N hexane, by Sinopharm (2 mentions)
Sodium hydroxide (naoh), by Sinopharm (2 mentions)
Ethanol, by Sinopharm (2 mentions)
Glacial acetic acid, by Sinopharm (2 mentions)
Acetonitrile, by Sinopharm (2 mentions)
O 7 azabenzotriazol 1 yl n n n n tetramethyluronium hexafluorophosphate hatu, by J&K Scientific (1 mentions)
Glutathione gsh, by J&K Scientific (1 mentions)
N n diisopropylethylamine, by J&K Scientific (1 mentions)
Acrylamide, by Adamas (1 mentions)
N n methylenebisacrylamide, by Merck Group (1 mentions)
N n n n tetramethylethylenediamine, by Merck Group (1 mentions)
N n dimethylformamide (dmf), by Merck Group (1 mentions)
Ammonium sulfate, by Sangon (1 mentions)
Streptomycin, by Beyotime (1 mentions)
Penicillin, by Beyotime (1 mentions)
Dimethylsulfoximine dmso, by Merck Group (1 mentions)
11 protocols using ethyl ether
1
Versatile Multifunctional Lipid Nanoparticles
2
Gynostemma pentaphyllum Therapeutic Potential
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Gynostemma pentaphyllum (Thumb) Makino herb was bought from a local drugstore of Zhengzhou (China). Caco-2 cells (HTB037) were acquired from the American Type Culture Collection and stored in our laboratory (13 (link)). Specific pathogen-free Kunming male mice (SCXK 2017-0002, 18–22 g) were bought from the Experimental Animal Center of Henan province (China). Dimethyl sulfoximine (DMSO), Dulbecco's modified Eagle's medium (DMEM), and 3-(4,5-Dimethylthiazol-2-yl)-2,5-bromo diphenyltetrazolium were purchased from Sigma-Aldrich (Shanghai, China). Fetal bovine serum, penicillin, and streptomycin were bought from Beyotime Biotechnology Co., Ltd., (Shanghai, China). Ethyl alcohol, sodium chloride, ethyl ether, sulfuricacid, calcium chloride, acetate, propionate, butyrate, and other chemical reagents were bought from Sinopharm Chemical Reagent Co., Ltd., (Beijing, China).
3
Analytical Reagents for Neuropharmacology Research
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The analytical reagents ethanol, ethyl acetate (EtOAc), petroleum ether, and ethyl ether and HPLC grade acetonitrile were purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Silica gel (300–400 mesh) was obtained from Qingdao Haiyang Chemical Co., Ltd (Qingdao, China). Phenytoin sodium, diazepam, PTZ, and pentobarbital sodium were purchased from Sigma (St. Louis, MO) and Aladdin Reagent Inc., (Shanghai, China). GABAA, GABAB, Bcl-2, and caspase-3 monoclonal antibodies were purchased from Abcam Co., Ltd (Cambridge, UK). Goat anti-rabbit/ thorseradish peroxidase-conjugated antibodies was purchased from Shanghai Hao Yang Biotechnology Co., Ltd (Shanghai, China).
4
Antioxidant Capacity Assays for Lotus Seeds
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Flash-frozen fresh lotus seeds were obtained from Green Acres Food Co., ltd. (Fujian, China). Coomassie brilliant blue protein reagent was obtained from Beijing Solarbio Technology Co., ltd., 95 % ethanol was obtained from Xilong Scientific Co., ltd., phenol, ethyl ether, and concentrated sulfuric acid were obtained from Sinopharm Group Chemical Reagent Co., ltd., Folin-Ciocalteu, anhydrous sodium carbonate, and catechol were obtained from Shanghai Macklin Biochemical Technology Co., ltd., AAPH (2, 2′-azobis(2-amidinopropane)dihydrochloride) was obtained from Qiaoyi Biotechnology (Shanghai) Co., Trolox (6-hydroxy-2, 5, 7, 8-tetramethylchroman-2-carboxylic acid) was obtained from Aldrich Chemical Co., TPTZ, and sodium fluorescein was purchased from Sigma Chemical Co. All other chemicals used were of analytical grade.
5
Extraction and Characterization of Paprika Pigments
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Paprika red pigment E150 (>98%) was provided by M&G Biotechnology Group Co., Ltd. (Handan, China). Corn starch (>99%) was purchased from Yishui Dadi Corn Development Co., Ltd. (Yishui, China). Potato starch (>99%) was purchased from Yantai Shuangta Food Co., Ltd. (Yantai, China). Amylopectin was obtained from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Amylose was purchased from Sigma-Aldrich Co., Ltd. (St. Louis, MO, USA). Methanol (MeOH) (Chromatographic grade) and methyl tert-butyl ether (MTBE) (Chromatographic grade) were purchased from Beijing Mairuida Technology Co., Ltd. (Beijing, China). Capsanthin standard sample (>97%), capsorubin standard sample (>97%), zeaxanthin standard sample (>97%), and β-cryptoxanthin standard sample (>97%) were obtained from Shanghai Huicheng Biotechnology Co., Ltd. (Shanghai, China). β-Carotene standard sample (>97%) was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). The reagents acetone, n-hexane, and ethyl ether were of analytical grade and were purchased from Sinopharm Group Chemical Reagent Co., Ltd. (Shanghai, China). Sodium chloride, anhydrous sodium sulfate, sodium hydroxide, potassium iodide, and iodine were purchased from Fuchen (Tianjin) Chemical Reagent Co., Ltd. (Tianjin, China).
6
Osteoclast Differentiation Protocol
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Rink Amide MBHA resin (0.3 mmol g−1 loading) was purchased from Tianjin Nankai Hecheng Science & Technology Co. Ltd (Tianjing, China). Fmoc-protected amino acids were purchased from Shanghai GL Biochem Ltd (Shanghai, China). HCTU, phenol, DIC, TIPS were obtained from adamas-beta (Shanghai, China). DMF, DCM were purchased from Wohua Chemical Co. Ltd (Shanghai, China). Ethyl ether, NMP, TFA, piperidine and other common reagent were purchased from Sinopharm Chemical Reagent Co. Ltd (Shanghai, China). The alpha modification of Eagle's medium (α-MEM), penicillin/streptomycin and fetal bovine serum (FBS) were purchased from Gibco-BRL (Gaithersburg, MD, USA). The cell counting kit-8 (CCK-8) was obtained from Dojindo Molecular Technology (Kumamoto, Kyushu, Japan). Recombinant mouse M-CSF and mouse RANKL were obtained from R&D (Minneapolis, MN, USA). The tartrate-resistant acid phosphatase (TRAP) staining kit were purchased from Joytech Bio Inc (Shaoxing, Zhejiang, China). Specific antibodies against phospho-GSK3β, GSK3β and GAPDH were obtained from Abcam (Cambridge, UK). Secondary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). RIPA lysis buffer, PMSF and SDS-PAGE loading buffer were purchased from Beyotime (Shanghai, China).
7
Cellulose and Lignin Extraction from Corncob Waste
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Benzyl-trimethyl ammonium hydroxide (BzMe3NOH; 40 wt% of aqueous solution) was purchased from TCI Shanghai Co. Ltd. (Shanghai, China). Ethylene glycol (98 wt%), tert-butanol (≥99.5%), and anhydrous calcium chloride (CaCl2; 96%) were supplied by Shanghai Macklin Biochemical Co. Ltd. (Shanghai, China). Glacial acetic acid, sodium acetate, sulfuric acid, hydrochloric acid, dioxane, acetone, ethyl ether, N,N-dimethylformamide, ethanol, sodium hydroxide, and urea were purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). Cellulose and lignin were extracted from corncob waste (Notes S1 and S2 ).
8
Synthesis and Characterization of Curcumin-Loaded Copolymer
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2-Ethyl-2-oxazoline (EtOx, Alfa, 99%) and dithioethylene glycol (HES, Alfa, 98%) that had been dried over calcium hydride and distilled under reduced pressure. Lactide (LA) had been recrystallized by toluene. Under nitrogen protection, toluene was dried under reflux by adding sodium wire. Chlorobenzene, N,N-dimethylformamide (DMF), acetonitrile, dichloromethane (DCM) were all anhydrous. Curcumin (Cur, 99%) was purchased from Beijing Zhongshuo Pharmaceutical Technology Development Co., Ltd. Methyl p-toluenesulfonate, potassium thiosulphonate (97%), 2,2′-dithiopyridine (98%) were supplied by Sean. Dithiothreitol (DTT, 99%) was bought from Merck. Concentrated hydrochloric acid, glacial acetic acid, potassium hydroxide, ethyl ether, and pyridine were purchased from Sinopharm Chemical Reagent Co., Ltd. and used directly.
Roswell Park Memorial Institute medium (RPMI-1640, Thermo Fisher Scientific Co., Ltd.), fetal bovine serum (FBS, Gibco), 3-(4,5-dimethyl-2-thio-2,5-diphenyl-2H-bromotetrazolium bromide tetrazolium) (MTT, Biosharp). Cell culture flasks were purchased from Corning Costar (Shanghai, China). 4′,6-Diamidino-2-phenylindole (DAPI, Roche). All other chemicals were used directly.
Roswell Park Memorial Institute medium (RPMI-1640, Thermo Fisher Scientific Co., Ltd.), fetal bovine serum (FBS, Gibco), 3-(4,5-dimethyl-2-thio-2,5-diphenyl-2H-bromotetrazolium bromide tetrazolium) (MTT, Biosharp). Cell culture flasks were purchased from Corning Costar (Shanghai, China). 4′,6-Diamidino-2-phenylindole (DAPI, Roche). All other chemicals were used directly.
9
Graphene Synthesis via Chemical Reduction
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Hydrochloric acid (HCl, 36–38%), sulfuric acid (H2SO4, 98%), phosphoric acid (H3PO4, 85%), ethanol (CH3CH2OH, 99.5%), acetone (CH3COCH3), ethyl ether (CH3CH2OCH2CH3), hydrogen peroxide (H2O2, 30%) and potassium permanganate (KMnO4, 99.5%) were obtained from Sinopharm Chemical Reagent Beijing Co., Ltd. Natural graphite powder (325 mesh, 99%) was obtained from Qingdao Hua Tai Lubricant Sealing S&T Co., Ltd. (Qingdao, China). Sodium hydroxide (NaOH, 99%) was obtained from Xi Long Chemical Co., Ltd. Dopamine hydrochloride [(OH)2C6H3CH2CH2NH2 HCl, 99%] was obtained from J&K Scientific Ltd. The above reagents were of analytical grade, and no further purification was performed before use. Copper sheets (99.95%) (10 mm × 10 mm × 0.1 mm) were obtained from Beijing Nonferrous Metal Research Institute. Thermally conductive silicone was obtained from Qingdao Hua Tai Lubricant Sealing S&T Co., Ltd. (Qingdao, China). Deionized water was used during the experiments.
10
Synthesis and Characterization of Fluorescent Dyes
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The RhB (>99.0%), Rh6G (>99.0%), Rh123 (>99.0%), sunset yellow (>95.0%), Congo red (>98.0%), fuchsin (>90.0%), neutral red (>90.0%), and 2,2′-azobis-1-vinylimidazole (>99.0%) were obtained from Shanghai Aladdin Bio-Chem Technology Co., Ltd (China). The RhB standard (DC16813550) was purchased from national standard network. All other chemicals and reagents (methyl-n-butyl-bromide, n-amyl-bromide, n-hexane bromide alcohol, ethyl ethanol, acetone, ethyl acetate, hydrochloric acid, sodium hydroxide, benzene, acetonitrile, chloroform, n-hexane, ethyl ether, potassium hexafluorophosphate, sodium chloride, magnesium sulfate, zinc chloride, iron sulfate) were of analytical grade and purchased from Sinopharm Chemical Reagent Co., Ltd (China). Aqueous solutions were prepared by using ultra-pure water obtained from a Milli-Q ultra-pure water generator.
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