Hematoxylin and eosin h e staining kit

The tibialis anterior (TA) muscle of mice was embedded with OCT and solidified at low temperature. The frozen sections were prepared into 10 μm and fixed with 4% paraformaldehyde (Beyotime, Beijing, China) for 30 min. Then, the sections were stained according to the instructions of the hematoxylin and eosin (H&E) staining kit (Solarbio, Beijing, China). Finally, the sections were observed and photographed under a microscope.

Thirty-seven, clean-grade, male Wistar rats, weighing 150–180 g were provided by the Experimental Animal Center of Southern Medical University (license number: SCXK [Guangdong] 2006-0015). The rats were reared in natural light conditions at a humidity of 50–60% and a constant temperature of 20 ± 3°C. Before the experiment, all rats were housed with food and water, freely available for 1 week, so they could adapt to the environment. Adenine (dissolved in normal saline before use) was purchased from Amresco Inc (Solon, OH, USA). APS (purity, 70%; freshly dissolved in 0.5% sodium carboxymethylcellulose [CMCNa] in saline before use) and Rhein (purity, 98%; freshly dissolved in CMCNa before use) were purchased from Nanjing Zelang Medical Technology Co., Ltd. (Nanjing, China). A hematoxylin and eosin (HE) staining kit was purchased from Solarbio (Beijing, China). A terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) apoptosis kit was purchased from Dingguo Changsheng Biotechnology Co., Ltd. (Beijing, China).

Chitosan (Cs), sodium alginate (Alg), and kappa carrageenan (κ-Car) were obtained from Sigma-Aldrich (St. Louis, MO, United States). Dextran sodium sulfate (DSS) was supplied from MP Biomedicals (Irvine, CA, United States). MSZ, β-CD, and HP-β-CD were purchased from Solarbio (Beijing, China). Male C57BL/6 mice (8 week old, 18–22 g) were provided by Pengyue Laboratory Animal Technology Co., Ltd. (Jinan, China). Myeloperoxidase (MPO) kits were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The Cell Counting Kit-8 (CCK-8) was obtained from Dongren Chemical Technology Co., Ltd. (Shanghai, China). The hematoxylin and eosin (H&E) staining kit was purchased from Solarbio (Beijing, China). The total antioxidant capacity assay kit with the ABTS method was obtained from Beyotime Biotechnology. Rhodamine B isothiocyanate (RBITC) was purchased from Macklin (Shanghai, China).

Ethylenediamine tetraacetic acid (EDTA), 4 % paraformaldehyde, HSA, Coomassie brilliant blue G250 and hematoxylin and eosin (H&E) staining kit were purchased from Beijing Solarbio Technology Co., Ltd (China). 4′,6-Diamidino-2-phenylindole Dihydrochloride (DAPI), trypan blue and glutathione (GSH) were obtained from Sigma-Aldrich Chemical Co. (USA). SMCC and 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB) were purchased from Adamas-beta (Shanghai, China). BCA protein quantitation kit and cell freezing medium were purchased from Beyotime Biotechnology Co., Ltd. (China). DMEM medium, RPMI 1640 medium, phosphate buffer saline (PBS), fetal bovine serum (FBS), penicillin and streptomycin were purchased from Gibco (USA). Interleukin-4 (IL-4) and Granulocyte-macrophage colony-stimulating factor (GM-CSF) were purchased from Wolcavi Biotech (Beijing, China). Mouse interferon-gamma (IFN-γ) enzyme-linked immunospot (ELISpot) plus kit was purchased from Mabtech (Sweden). Anti-CD11c-APC, anti-CD80-PE and anti-CD86-PE-Cy7 were purchased from Thermo Fisher Scientific (USA). Anti-CD3-APC and anti-CD8-PE were purchased from BioLegend, Inc (USA). Mouse IFN-γ enzyme-linked immunosorbent assay (ELISA) kit, mouse interferon beta (IFN-β) ELISA kit and mouse tumor necrosis factor alpha (TNF-α) ELISA kit were purchased from Fankewei (Shanghai, China).

Limonin (C₃₂H₄₂O₁₄, purity ≥98%) was purchased from Solarbio (Beijing, China). Diaminobenzidine (DAB) developer, pentobarbital sodium, and the hematoxylin and eosin (H&E) staining kit were provided by Solarbio (Beijing, China). Rabbit anti–cadherin 5 was purchased from Boster Biological Technology (A02632–2). Rabbit anti-GAPDH was acquired from Biogot Technology (AP0063). Rabbit anti–vascular endothelial growth factor (VEGF), anti–superoxide dismutase 1 (SOD1), anti–matrix metalloproteinase 9 (MMP9), anti-HO-1, and anti-CAPS3 were acquired from Proteintech (19003-1, 10269-1, 10375-2, 21327-1, and 19677-1). Rabbit anti–endothelial nitric oxide synthase (eNOS), anti–cytochrome c (CYC), and anti-Bax were purchased from Cell Signaling Technology (12994, 14796, and 32027). Horseradish peroxidase (HRP)–conjugated immunoglobulin G (IgG) secondary antibody was purchased from Santa Cruz Biotechnology. Fluorescein isothiocyanate (FITC)–conjugated IgG secondary antibody was obtained from Boyun Biotechnology, and the 4′,6-diamidino- 2-phenylindole (DAPI) solution was purchased from Beyotime Biotechnology. The Electrochemiluminescence (ECL) Plus Reagent Kit was obtained from PerkinElmer Life Sciences and the BCA kit was acquired from TermoFisher Scientifc.