Log In Sign up
The largest database of trusted experimental protocols

Fxr1 antibody

Manufactured by Cell Signaling Technology
Visit Supplier

The FXR1 antibody is a reagent used for the detection and analysis of the FXR1 protein, which is a member of the fragile X-related protein family. This antibody can be used in various analytical techniques, such as Western blotting, immunoprecipitation, and immunocytochemistry, to investigate the expression and localization of the FXR1 protein in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Plko 1, by Merck Group (2 mentions) Ddx17, by Abcam (1 mentions) Streptavidin magnetic beads, by Promega (1 mentions) Dhx36, by Abcam (1 mentions) Rna oligonucleotides, by Integrated DNA Technologies (1 mentions) Anti myc, by Abcam (1 mentions)

3 protocols using fxr1 antibody

1

Generating Stable FXR1 Knockdown Cell Line

Check if the same lab product or an alternative is used in the 5 most similar protocols
For viral creation, HEK293T cells were transfected with packaging vectors pLP1, pLP2 and VSVG plasmids including control empty vector pLKO.1 (Cat#SHC001V) and two different FXR1 targeting short hairpin RNA (shRNA) (TRC number 1: TRCN0000160812, Clone ID: NM_005087.1-130s1c1; 2: TRCN0000160901, Clone ID: NM_005087.1-579s1c1) purchased from Sigma-Aldrich (Saint Louis, MO). Competent lentiviruses were collected 48 h after transfection. HeyA8 cells were passaged to 40% confluence, the next day viral media were added to cells with 8 μg/ml of polybrene. Efficacy of individual FXR1 shRNA construct was checked by western blot analysis for FXR1 knockdown using FXR1 antibody (Cat#12295, Cell Signaling Technology). The most effective shRNA construct was used for generating FXR1 knockdown stable cell line by selection with puromycin (8 μg/ml; for 2 weeks). The clones were picked and subjected to expansion culture under further selection. Western blot analysis was performed to identify the stable clone with most efficiently downregulated FXR1 protein, which was used in further experiments.
George J., Li Y., Kadamberi I.P., Parashar D., Tsaih S.W., Gupta P., Geethadevi A., Chen C., Ghosh C., Sun Y., Mittal S., Ramchandran R., Rui H., Lopez-Berestein G., Rodriguez-Aguayo C., Leone G., Rader J.S., Sood A.K., Dey M., Pradeep S, & Chaluvally-Raghavan P. (2021). RNA-binding protein FXR1 drives cMYC translation by recruiting eIF4F complex to the translation start site. Cell reports, 37(5), 109934.
+ Open protocol
+ Expand
2

Affinity Purification of RNA Interactors

Check if the same lab product or an alternative is used in the 5 most similar protocols
Anti-MYC, Hemagglutinin tag, DHX36, DDX5, DDX17, DHX9, DHX29 and DHX30 antibodies were purchased from Abcam, the V5-tag antibody was obtained from Source BioScience, DDX3X antibody was ordered from Santa Cruz and FXR1 antibody was purchased from Cell Signaling. RNA oligonucleotides were ordered from Integrated DNA Technologies. Streptavidin magnetic beads were obtained from Promega and Strep-Tactin magnetic nanobeads were purchased from IBA.
Herdy B., Mayer C., Varshney D., Marsico G., Murat P., Taylor C., D'Santos C., Tannahill D, & Balasubramanian S. (2018). Analysis of NRAS RNA G-quadruplex binding proteins reveals DDX3X as a novel interactor of cellular G-quadruplex containing transcripts. Nucleic Acids Research, 46(21), 11592-11604.
+ Open protocol
+ Expand
3

Generating Stable FXR1 Knockdown Cell Line

Check if the same lab product or an alternative is used in the 5 most similar protocols
For viral creation, HEK293T cells were transfected with packaging vectors pLP1, pLP2 and VSVG plasmids including control empty vector pLKO.1 (Cat#SHC001V) and two different FXR1 targeting short hairpin RNA (shRNA) (TRC number 1: TRCN0000160812, Clone ID: NM_005087.1-130s1c1; 2: TRCN0000160901, Clone ID: NM_005087.1-579s1c1) purchased from Sigma-Aldrich (Saint Louis, MO). Competent lentiviruses were collected 48 h after transfection. HeyA8 cells were passaged to 40% confluence, the next day viral media were added to cells with 8 μg/ml of polybrene. Efficacy of individual FXR1 shRNA construct was checked by western blot analysis for FXR1 knockdown using FXR1 antibody (Cat#12295, Cell Signaling Technology). The most effective shRNA construct was used for generating FXR1 knockdown stable cell line by selection with puromycin (8 μg/ml; for 2 weeks). The clones were picked and subjected to expansion culture under further selection. Western blot analysis was performed to identify the stable clone with most efficiently downregulated FXR1 protein, which was used in further experiments.
George J., Li Y., Kadamberi I.P., Parashar D., Tsaih S.W., Gupta P., Geethadevi A., Chen C., Ghosh C., Sun Y., Mittal S., Ramchandran R., Rui H., Lopez-Berestein G., Rodriguez-Aguayo C., Leone G., Rader J.S., Sood A.K., Dey M., Pradeep S, & Chaluvally-Raghavan P. (2021). RNA-binding protein FXR1 drives cMYC translation by recruiting eIF4F complex to the translation start site. Cell reports, 37(5), 109934.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!