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Oxymax lab animal monitoring system

Manufactured by Columbus Instruments
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The Oxymax Lab Animal Monitoring System is a product developed by Columbus Instruments. It is a comprehensive system designed to measure various physiological parameters of laboratory animals, including oxygen consumption, carbon dioxide production, and energy expenditure. The system provides real-time data collection and analysis capabilities to support research and scientific investigations.

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Lab products found in correlation

5520 vapor pressure osmometer, by Wescor (1 mentions) Echomri 3 in 1 system nuclear magnetic resonance spectrometer, by Echo Medical Systems (1 mentions) C57bl 6j mice, by Central Lab Animal (1 mentions) Rnalater stabilization solution am7020, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Oxymax (120 citations) Oxymax system (90 citations)

15 protocols using oxymax lab animal monitoring system

1

Metabolic Profiling in Mice

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Following eight weeks of SI (or control housing), a subset of mice (n = 4 per group) was individually placed in the chambers of the Oxymax Lab Animal Monitoring system (Columbus Instruments, OH). Following 2 days of acclimatization, food intake, activity, heat generated, and respiratory exchange ratio (RER) were recorded in day 3.
Sun M., Choi E.Y., Magee D.J., Stets C.W., During M.J, & Lin E.J. (2014). Metabolic Effects of Social Isolation in Adult C57BL/6 Mice. International Scholarly Research Notices, 2014, 690950.
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2

Smooth Muscle PPARγ Activation Phenotyping

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Littermate control and smPPARγOE mice were subjected to a broad variety of studies to establish the impact of smooth muscle-targeted PPARγ activation on phenotype. Food and water consumption were measured over 24 hours in individually-housed mice. Glucose tolerance tests were performed by injecting mice with intraperitoneal glucose (2 g/kg body weight) then determining glucose concentration of blood obtained from the tail vein at 0, 20, 40, 60, 120, and 240 minutes using an Accu-Chek Avia meter. Urine osmolality was measured on a Wescor 5520 Vapor Pressure Osmometer (Wescor, Logan, UT). Urinary sodium, chloride, and potassium were measured by the EasyLyte (Medica, Bedford, MA) instrument. Urine creatinine was determined with a kit by the Jaffe reaction method (BioVision, Milpitas, CA). Metabolic rates were measured on individual mice with the Oxymax Lab Animal Monitoring System (Columbus Instruments, Columbus, OH).
Kleinhenz J.M., Murphy T.C., Pokutta-Paskaleva A.P., Gleason R.L., Lyle A.N., Taylor W.R., Blount M.A., Cheng J., Yang Q., Sutliff R.L, & Hart C.M. (2015). Smooth Muscle-Targeted Overexpression of Peroxisome Proliferator Activated Receptor-γ Disrupts Vascular Wall Structure and Function. PLoS ONE, 10(10), e0139756.
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3

Comprehensive Metabolic Assessment in Mice

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To evaluate systemic energy metabolism, mice treated according to the prevention and early reversibility protocols were acclimated to single cages during a 24-hour period. Thereafter, Scr- and ASO-treated mice from both experimental designs were monitored for four consecutive days in the Oxymax Lab Animal Monitoring System [Comprehensive Laboratory Animal Monitoring System (CLAMS), Columbus Instruments] with ad libitum access to food except for a 12-hour fasting period during the last night. The following parameters were continuously monitored: calorie intake, locomotion, VO2, VCO2, and RER. Locomotion was reported as total beam breaks for the x and y axes. Energy expenditure (EE) was calculated as previously described (30 –32 ) using the following formula: EE (kcal/kg per hour ) = (3.815 + 1.232 × RER) × VO2. In vivo FAO was calculated as previously described (30 –32 ) using the following formula: FAO (kcal/kg per hour) = EE × (1 – RER/0.3).
Valladolid-Acebes I., Åvall K., Recio-López P., Moruzzi N., Bryzgalova G., Björnholm M., Krook A., Alonso E.F., Ericsson M., Landfors F., Nilsson S.K., Berggren P.O, & Juntti-Berggren L. (2021). Lowering apolipoprotein CIII protects against high-fat diet–induced metabolic derangements. Science Advances, 7(11), eabc2931.
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4

Ambulatory Activity in AgRP Mice

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10–12 weeks old male AgRP DTR and DTR mice were acclimatized to the experimental chambers for at least 24 h prior to data collection using the Oxymax Lab Animal Monitoring System (Columbus Instruments). Only ambulatory beam breaks in the x and y axis were included in the analysis.
Tan K., Knight Z.A, & Friedman J.M. (2014). Ablation of AgRP neurons impairs adaption to restricted feeding. Molecular Metabolism, 3(7), 694-704.
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5

Metabolic Profiling of Tgr5 Knockout Mice

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Comprehensive metabolic profiling of 20-21 week old male WT and Tgr5−/− mice during 24 h fasting was performed using an Oxymax lab animal monitoring system (Columbus Instruments, Columbus, OH). Respiratory exchange ratio, O2 consumption and CO2 production, activity and energy expenditure of mice were measured during 24 h fasting.
Donepudi A.C., Boehme S., Li F, & Chiang J.Y. (2016). G protein-coupled bile acid receptor plays a key role in bile acid metabolism and fasting-induced hepatic steatosis. Hepatology (Baltimore, Md.), 65(3), 813-827.
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6

Metabolic Profiling of Mice

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Bodyweight and body composition were measured in ad-lib fed conscious mice using an EchoMRI™ 3-in-1 system nuclear magnetic resonance spectrometer (Echo Medical Systems, Houston, TX) to determine whole-body lean and fat mass. Mice were individually placed in the Oxymax Lab Animal Monitoring System (Columbus Instruments) with free access to food and water. After overnight acclimation, oxygen consumption, the amount of carbon dioxide produced, food and liquid intake, and locomotor activity (beam breaks) were determined. Respiratory exchange ratios were calculated by dividing the volume of carbon dioxide produced by the volume of oxygen consumed (VCO2/VO2).
Kumar A., Sundaram K., Mu J., Dryden G.W., Sriwastva M.K., Lei C., Zhang L., Qiu X., Xu F., Yan J., Zhang X., Park J.W., Merchant M.L., Bohler H.C., Wang B., Zhang S., Qin C., Xu Z., Han X., McClain C.J., Teng Y, & Zhang H.G. (2021). High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance. Nature Communications, 12, 213.
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7

Thermoneutral Indirect Calorimetry in Mice

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Blood glucose levels were measured using a blood glucose meter (LifeScan Inc., Milpitas, CA). Indirect calorimetry was performed 5 weeks postoperatively. The Oxymax Lab Animal Monitoring System (CLAMS) was used (Columbus Instruments, OH, USA). All mice were placed in the cages of the indirect calorimeter system for acclimation before experimental measurements. The CLAMS system is located in a room where ambient temperature can be controlled and the temperature inside the cages was documented to be always 29 °C. This temperature is in the thermoneutrality zone for mice, so all measurements were performed under thermoneutral conditions. Measurements were performed for 72 hours during which animals had access to HFD and water ad libitum. Oxygen consumption, carbon dioxide production and heat production were recorded for each animal. Resting energy expenditure was calculated by correlating oxygen consumption, food intake and activity based on the recordings of the metabolic system.
Ben-Zvi D., Meoli L., Abidi W.M., Nestoridi E., Panciotti C., Castillo E., Pizarro P., Shirley E., Gourash W.F., Thompson C.C., Munoz R., Clish C.B., Anafi R.C., Courcoulas A.P, & Stylopoulos N. (2018). Time-Dependent Molecular Responses Differ Between Gastric Bypass and Dieting But Are Conserved Across Species. Cell metabolism, 28(2), 310-323.e6.
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8

Metabolic Profiling of Nrf2 KO Mice

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All animal studies were carried out in accordance with the guidelines of the Animal Research Committee (SKKUIACUC2020- 08-21-1) of Sungkyunkwan University. Seven weeks old male C57BL/6J mice were purchased from Central Lab Animal Inc (Seoul, KoreA). and housed in rooms at ambient temperature. The Nrf2 KO mice were previously described (35 (link)). Whole-body energy metabolism was measured using the Columbus Instruments Oxymax Lab Animal Monitoring System. The mice were placed in metabolic cages and acclimated in the metabolic chambers for one day before measuring O2 consumption.
Chang S.H., Jang J., Oh S., Yoon J.H., Jo D.G., Yun U.J, & Park K.W. (2021). Nrf2 induces Ucp1 expression in adipocytes in response to β3-AR stimulation and enhances oxygen consumption in high-fat diet-fed obese mice. BMB Reports, 54(8), 419-424.
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9

Indirect Calorimetry for Energy Expenditure

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Oxymax lab animal monitoring system (Columbus Instruments, Columbus, OH, USA) was employed to determine energy expenditures. After receiving Ft1 for 6 weeks, each group mice were kept in the separated metabolic chamber for 24 h to adapt the environment. Oxygen consumption volume (VO2) and CO2 release were determined during a 24 h period. Respiratory quotient (RQ) equals volumes of CO2 production/volumes of O2 consumption. Results are exhibited for the last 12 h of the light cycle and 12 h of the dark cycle over both dark and light phases.
Ding L., Yang Q., Zhang E., Wang Y., Sun S., Yang Y., Tian T., Ju Z., Jiang L., Wang X., Wang Z., Huang W, & Yang L. (2021). Notoginsenoside Ft1 acts as a TGR5 agonist but FXR antagonist to alleviate high fat diet-induced obesity and insulin resistance in mice. Acta Pharmaceutica Sinica. B, 11(6), 1541-1554.
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10

Postnatal Brain and Liver Fatty Acid Analysis

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All the mice were fed ad libitum laboratory rodent chow (7012 Teklad LM-485, Harlan Laboratories, WI) containing 2.6%wt linoleic acid (7.6%energy) and 0.3%wt α-linolenic acid (0.9%energy) from soy oil. They were housed in a controlled environment at a 12-hour light/12-hour dark cycle. Body weights were measured three times per week. Oxymax Lab Animal Monitoring System (Columbus Instruments, OH) was used to test physical activities, specifically respiratory exchange ratio, horizontal activity (count/day), vertical activity (count/day), food intake (g/day), and water intake (ml/day). Pups (n=14/genotype/age, female: male= 1:1) on postnatal day 1, 3, 7, 13, 21, and 30 were sacrificed by CO2 inhalation and brain and liver tissues were collected for fatty acid analysis and gene expression studies. Tissues were kept in RNAlater stabilization solution (AM7020, Life Technologies, CA) at 4°C overnight and the next day stored at −80°C until use. Animal studies were approved by the Cornell University Institutional Animal Care and Use Committee (IACUC protocol #2011-0007).
Zhang J.Y., Qin X., Liang A., Kim E., Lawrence P., Park W.J., Kothapalli K.S, & Brenna J.T. (2017). Fads3 modulates docosahexaenoic acid in liver and brain. Prostaglandins, leukotrienes, and essential fatty acids, 123, 25-32.
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