Sodium nitrite solution standard

Manufactured by Merck Group

Sourced in United States, China

Sodium nitrite solution standard is a laboratory reagent used as a reference material for analytical procedures. It provides a known concentration of sodium nitrite, which can be used to calibrate and verify the performance of analytical instruments and methods. The solution is typically supplied in a sealed container at a specified concentration.

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Lab products found in correlation

Griess reagent, by Merck Group (3 mentions) Microplate reader, by Molecular Devices (1 mentions)

Close spelling variants of this product

Sodium nitrite solution Nitrite standard Sodium nitrite Standard solutions

4 protocols using sodium nitrite solution standard

Quantifying Inflammatory Markers in Murine Serum

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The level of PGE2 production in the serum of mice was measured using a commercially available ELISA kit (cat no. 514010; Cayman Chemical, Ann Arbor, MI, USA) according to the manufacturer's instructions.
Nitrite accumulation in the serum of mice was measured colorimetrically by the Griess reaction using a Griess reagent (Sigma-Aldrich). For the assay, equal volumes of the serum of CIA mice and Griess reagent were mixed, and the absorption coefficient was calibrated using a sodium nitrite solution standard (Sigma-Aldrich). The absorbance of each sample after the Griess reaction was determined using a microplate reader at 540 nm.

Han H.M., Hong S.H., Park H.S., Jung J.C., Kim J.S., Lee Y.T., Lee E.W., Choi Y.H., Kim B.W., Kim C.M, & Kang K.H. (2016). Protective effects of Fructus sophorae extract on collagen-induced arthritis in BALB/c mice. Experimental and Therapeutic Medicine, 13(1), 146-154.

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Nitrite Measurement in LPS-Induced RAW 264.7 Cells

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The RAW 264.7 macrophage cells were plated at a density of 2 × 10⁵ cells per well in a 24-well plate. The cells were pre-treated with the indicated concentrations of various extracts for 2 h, and then induced with a 1 μg/mL concentration of LPS for an additional 22 h. Nitrite accumulation in the culture was measured colorimetrically by the Griess reaction using a Griess reagent (Sigma-Aldrich, St. Louis, MO, USA). For the assay, equal volumes of cultured medium and Griess reagent were mixed, and the absorption coefficient was calibrated using a sodium nitrite solution standard (Sigma-Aldrich, St. Louis, MO, USA). The absorbance of each sample after the Griess reaction was determined by an ELISA plate reader at 540 nm.

Kim C., Ji J., Ho Baek S., Lee J.H., Ha I.J., Lim S.S., Yoon H.J., Je Nam Y, & Ahn K.S. (2019). Fermented dried Citrus unshiu peel extracts exert anti-inflammatory activities in LPS-induced RAW264.7 macrophages and improve skin moisturizing efficacy in immortalized human HaCaT keratinocytes. Pharmaceutical Biology, 57(1), 392-402.

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Inhibition of LPS-induced NO by OMEO

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The inhibitory effect of OMEO on NO levels induced by LPS stimulation in RAW 264.7 cells was determined using Griess assay [8 (link)]. RAW 264.7 cells (3 × 10⁴ cells/well) were seeded into a 96-well plate and cultured for 24 h. After then, OMEO at various concentration (12.5–100 μg/mL) were treated with LPS (1 μg/mL) for additional 24 h. One hundred μL of supernatant of the medium were added to a new 96-well plate and mixed with 100 μL of the Griess reagent (Sigma-Aldrich). After 15 min incubation at room temperature, absorbance was measured using a microplate reader at 540 nm, and NO concentration was calculated using sodium nitrite standard solution (Sigma-Aldrich).

Lee J.H., Lee Y.Y., Lee J., Jang Y.J, & Jang H.W. (2021). Chemical Composition, Antioxidant, and Anti-Inflammatory Activity of Essential Oil from Omija (Schisandra chinensis (Turcz.) Baill.) Produced by Supercritical Fluid Extraction Using CO2. Foods, 10(7), 1619.

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Griess Assay for Nitrite Quantification

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Nitrite (a stable metabolite of nitric oxide, produced from polarized activation M1-like chicken macrophages) concentration in the cell culture supernatants was measured by the Griess assay as described previously (35 (link)). Briefly, 100 microliters of supernatants from each tested well was transferred to the 96-well ELISA plate and mixed with 50 µl of 0.1% naphthalenediamine and 50 µl of 1% sulfanilamide (both were prepared in 2.5% phosphoric acid solution). After incubation at 25°C for 10 min, the nitrite concentration was determined by measuring absorbance at 595 nm of each well in a microplate reader (Molecular Devices, Sunnyvale, CA, USA). Sodium nitrite standard solution (Sigma, Shanghai, China) also including in every ELISA plate.

Zhang P., Ding Z., Liu X., Chen Y., Li J., Tao Z., Fei Y., Xue C., Qian J., Wang X., Li Q., Stoeger T., Chen J., Bi Y, & Yin R. (2018). Enhanced Replication of Virulent Newcastle Disease Virus in Chicken Macrophages Is due to Polarized Activation of Cells by Inhibition of TLR7. Frontiers in Immunology, 9, 366.

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