Enzychrome triglyceride assay kit

Liver TG were determined using the EnzyChrome triglyceride assay kit (BioAssay Systems, Hayward, CA, USA). Tissue (10 mg) were homogenized in 100 μL of 5% Triton X‐100 and incubated in water bath for 5 min in which the temperature increases from 80 to 100 °C. The procedure was repeated three times, allowing the samples to settle at 20 °C between cycles. Samples were then centrifuged (16 000 g for 5 min at 4 °C), the supernatant was diluted 1 : 10 in MilliQ water (Millipore, Burlington, MA, USA) and 10 μL of this was used for the assay performed in triplicate in 96‐well plates. Samples were mixed with 100 μL of kit working reagent and incubated (15 min at 20 °C) before plates were read at 595 nm wavelength in a Bio‐Rad iMark microplate spectrophotometer.

Serum AST and ALT activities were determined on a Roche Modular System (Roche Diagnostics) with a commercially available automated colorimetric system at the Institute of Clinical Chemistry, University Hospital Zurich, using a Hitachi P-Modul (Roche). Triglyceride concentrations in mouse liver homogenates were measured using EnzyChrome triglyceride assay kit (BioAssay Systems) as previously described by us^{20 (link)}.