CD14 + cells were isolated from cryopreserved PBMCs. PBMCs were magnetic labeled with CD14 MicroBeads (Miltenyi Biotec) and isolated through positive selection using MACS Separation Columns MS (Miltenyi Biotec), according to the manufacturer’s protocol. Purified cells (~95% pure) were counted and plated in 96 well plates at a concentration of 0.5 × 106 cell mL–1 in 200 μl of cRPMI (GIBCO).
Macs separation ms columns
MACS separation MS columns are designed for magnetic cell separation. They provide a platform for the isolation and enrichment of target cells from heterogeneous cell populations. The columns leverage the power of magnetic beads coated with specific antibodies to selectively capture and separate the desired cell types.
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5 protocols using macs separation ms columns
Isolation and Culture of PBMCs
CD14 + cells were isolated from cryopreserved PBMCs. PBMCs were magnetic labeled with CD14 MicroBeads (Miltenyi Biotec) and isolated through positive selection using MACS Separation Columns MS (Miltenyi Biotec), according to the manufacturer’s protocol. Purified cells (~95% pure) were counted and plated in 96 well plates at a concentration of 0.5 × 106 cell mL–1 in 200 μl of cRPMI (GIBCO).
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