Apc pd 1

Manufactured by BioLegend

Sourced in United States

The APC-PD-1 is a fluorescently-labeled antibody that binds to the PD-1 (Programmed Cell Death Protein 1) receptor on the surface of cells. It is designed for use in flow cytometry applications to detect and quantify PD-1 expression on various cell types.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Anti-PD-1-APC (18 citations) PD-1-APC-Cy7 (9 citations) APC-conjugated anti-PD-1 (4 citations) APC-conjugated anti-human PD-1 (3 citations) PD-1 APC clone 29F.1A12 (2 citations) PD-1 APC (clone EH12.2H7; (2 citations) APC-conjugated anti-human PD-1 antibody (2 citations) Anti-PD-1 APC (clone 29F-1A12), (2 citations) Anti-PD-1-APC (clone EH12.2H7, (2 citations) Mouse anti-human PD-1-APC (2 citations)

4 protocols using apc pd 1

Comprehensive Immune Profiling of HIV-1 Infection

Check if the same lab product or an alternative is used in the 5 most similar protocols

HIV-1-infected PBMCs or purified CD4⁺ T cells were stained with fluorescently labeled anti-human antibodies (1:50 dilution) in the cell stain buffer (BioLegend) for 30 min at 4 °C, washed twice using the cell stain buffer, fixed in 2% paraformaldehyde (PFA), washed again once in PBS, and re-suspended in PBS before flow cytometry. Cell staining was quantified with a BD FACSCanto II cytometer. The data were analyzed using FlowJo software (version 10.7.1, Treestar). The following antibodies (BioLegend) were used for staining surface receptors: APC PD-1 (#329907), APC TIGIT (#372705), Alexa Fluor 647 LAG-3 (#369303), APC Tim-3 (#345012), APC-Cy7 Tim-3 (#345026), APC CD4 (#317416), and APC/Cy7 HLA-A, B, C (#311426). The following isotype controls were used in this study: APC Mouse IgGκ, Alexa Fluor 647 Mouse IgG1κ, APC Mouse IgG2aκ, APC Mouse IgG2bκ, APC/Cy7 Mouse IgG1κ, and APC/Cy7 Mouse IgG2aκ.

Jacob R.A., Edgar C.R., Prévost J., Trothen S.M., Lurie A., Mumby M.J., Galbraith A., Kirchhoff F., Haeryfar S.M., Finzi A, & Dikeakos J.D. (2021). The HIV-1 accessory protein Nef increases surface expression of the checkpoint receptor Tim-3 in infected CD4+ T cells. The Journal of Biological Chemistry, 297(3), 101042.

+ Open protocol

+ Expand

Isolation and Phenotyping of Human Visceral Adipose Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Visceral adipose tissue was collected from male bariatric surgery patients with IRB approval (HUM00074075) from the University of Michigan and Ann Arbor Veterans Affairs Healthcare System. We did not include or exclude any bariatric surgery samples based on race. Sex and racial classifications were made by the participant and extracted from the medical record.
Tissue was finely minced using surgical scissors (DR Instruments) and then digested in 3 mg/mL collagenase II (Invitrogen; 17101015) for 30 minutes. Digested tissue was then processed in the same manner as digested murine adipose tissue to obtain single-cell suspensions of SVF.
Cells were incubated in Fc Block for 5 minutes on ice before staining with indicated antibodies for 30 minutes at 4°C. Anti-human antibodies used included the following: AF488-CD4 (catalog 317419), PerCPy5.5-CD3 (catalog 300429), APC-CD25 (catalog 356109), APCcy7-CD8 (catalog 344713), APC-PD1 (catalog 329907), and PE-FoxP3 (catalog 320107) from BioLegend, and Live/Dead Fixable Dead Cell Violet Stain Kit (catalog L34955) from Invitrogen.

Porsche C.E., Delproposto J.B., Geletka L., O’Rourke R, & Lumeng C.N. (2021). Obesity results in adipose tissue T cell exhaustion. JCI Insight, 6(8), e139793.

+ Open protocol

+ Expand

Dissociation and Phenotyping of ccRCC Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Peritumour tissue and ccRCC tissue from ccRCC patients were individually dissociated in 50 U/mml of Dispase II (S25046, YuanyeBio-Technology, China) on gentleMACS octo with Heater (Miltenyi Biotec, German) to enable efficient dissociation of lymphocytes from the tumour cells and stromal tissue. Staining steps were performed at 4 ℃ over 20 min with FACS buffer washes between steps. Flow cytometry antibodies were used: FITC-CD3 (Cat: 317305, Clone: OKT3, 1:20, Biolegend), PE-IFN-γ (Cat: 502508, Clone: 4SB3, 1:20, Biolegend), APC-PD-1 (Cat: 379207, Clone: A17188A, 1:20, Biolegend).

Zhang W., Zhang Q., Zhu C., Shi Z., Shao C., Chen Y., Wang N., Jiang Y., Liang Q, & Wang K. (2022). The intrarenal landscape of T cell receptor repertoire in clear cell renal cell cancer. Journal of Translational Medicine, 20, 558.

+ Open protocol

+ Expand

Immune Cell Profiling of Mouse Tumors

Check if the same lab product or an alternative is used in the 5 most similar protocols

For mouse tissue, each tumor was minced using the mouse Tumor Dissociation Kit from Miltenyi Biotec (#130-096-730, Auburn, California, USA). All samples were then washed with flow cytometry buffer, and the cells were further passed through a 100 µm cell strainer. The samples were incubated for 30 min at 4°C in the dark with the following antibodies: APC-PD-1 (#135210), BV711-TIM3 (#134021), PE-IL-2 (#503808), FITC-IFN-γ (#505806), APC-Thy1.2 (#140331), Pacific Blue-Granzyme B (#515408), PE-TNFα (#506306), PE-CD4 (#100408), APC-perforin (#154304), APC-Annexin V (#640941) and FITC-CD8 (#100706) from BioLegend (San Diego, California, USA). Intracellular staining was performed after incubation of single-cell suspensions with BD GolgiStop from BD Biosciences (#AB_2869012, San Diego, California, USA) in medium for 4 hours using Intracellular Staining Permeabilization Wash Buffer and Fixation Buffer from BioLegend (#421002, San Diego, California, USA). Stained cell populations were acquired by LSRFortessa from BD Biosciences (Franklin, New Jersey, USA), and the results were analyzed by using FlowJo software from Tree Star (Ashland, Oregon, USA).

Ren Y., Kumar A., Das J.K., Peng H.Y., Wang L., Balllard D., Xiong X., Ren X., Zhang Y., Yang J.M, & Song J. (2022). Tumorous expression of NAC1 restrains antitumor immunity through the LDHA-mediated immune evasion. Journal for Immunotherapy of Cancer, 10(9), e004856.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!