Gfap z0334

Manufactured by Agilent Technologies

Sourced in United States

GFAP; Z0334 is a laboratory reagent used for the detection and quantification of glial fibrillary acidic protein (GFAP), a biomarker commonly associated with various neurological conditions. This product provides a reliable and standardized tool for researchers and clinicians to measure GFAP levels in biological samples.

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Lab products found in correlation

Benchmark ultra immunostainer, by Roche (2 mentions) Ultraview universal dab detection kit, by Roche (2 mentions) Clone mib 1, by Agilent Technologies (1 mentions) Optiview dab ihc detection kit, by Roche (1 mentions) Neun clone a60, by Merck Group (1 mentions) Cd34 clone qbend 10, by Roche (1 mentions)

Spelling variants of this product

Z0334 (169 citations) Anti-GFAP (Z0334, (7 citations) Rabbit anti-GFAP (Z0334) (5 citations)

2 protocols using gfap z0334

Histological Review and Immunohistochemical Analysis

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For all cases with sufficient material (n = 16), histological review of an H&E-stained slide was performed according to the World Health Organization (WHO) 2016 classification of tumors of the CNS [17 (link)]. Immunohistochemical staining was performed on a Ventana BenchMark ULTRA Immunostainer using the ultraView Universal DAB Detection Kit (Ventana Medical Systems, Tucson, AZ, USA). Antibodies were directed against: glial fibrillary acid protein (GFAP; Z0334, rabbit polyclonal, 1:1000 dilution, Dako Agilent, Santa Clara, CA, USA), epithelial membrane antigen (EMA; clone GP1.4, mouse monoclonal, dilution 1:1000, Thermo Fisher Scientific, Fremont, CA, USA), Sry-related HMG-BOX gene 10 (SOX10; clone EP268, rabbit monoclonal, dilution 1:100, Cell Marque Corp., Rocklin, CA, USA) and oligodendrocyte lineage transcription factor 2 (OLIG2; clone EPR2673, rabbit monoclonal, dilution 1:50, Abcam, Cambridge, UK).

Sievers P., Henneken S.C., Blume C., Sill M., Schrimpf D., Stichel D., Okonechnikov K., Reuss D.E., Benzel J., Maaß K.K., Kool M., Sturm D., Zheng T., Ghasemi D.R., Kohlhof-Meinecke P., Cruz O., Suñol M., Lavarino C., Ruf V., Boldt H.B., Pagès M., Pouget C., Schweizer L., Kranendonk M.E., Akhtar N., Bunkowski S., Stadelmann C., Schüller U., Mueller W.C., Dohmen H., Acker T., Harter P.N., Mawrin C., Beschorner R., Brandner S., Snuderl M., Abdullaev Z., Aldape K., Gilbert M.R., Armstrong T.S., Ellison D.W., Capper D., Ichimura K., Reifenberger G., Grundy R.G., Jabado N., Krskova L., Zapotocky M., Vicha A., Varlet P., Wesseling P., Rutkowski S., Korshunov A., Wick W., Pfister S.M., Jones D.T., von Deimling A., Pajtler K.W, & Sahm F. (2021). Recurrent fusions in PLAGL1 define a distinct subset of pediatric-type supratentorial neuroepithelial tumors. Acta Neuropathologica, 142(5), 827-839.

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Histopathological Characterization of CNS Tumors

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All samples with available tissue (n = 24/30) were histopathologically re-assessed according to the WHO 2016 classification of tumors of the central nervous system. Formalin-fixed, paraffin-embedded (FFPE) tissue samples were stained with hematoxylin and eosin (H&E) according to standard protocols. For all cases with sufficient material, immunohistochemistry was performed on a Ventana BenchMark ULTRA Immunostainer using either the OptiView DAB IHC Detection Kit or the ultraView Universal DAB Detection Kit (Ventana Medical Systems, Tucson, Arizona, USA). Antibodies were directed against: glial fibrillary acid protein (GFAP; Z0334, rabbit polyclonal, 1:1000 dilution, Dako Agilent, Santa Clara, CA, USA), Olig2 (clone EPR2673, rabbit monoclonal, 1:100 dilution, Abcam, Cambridge, UK), Synaptophysin (clone MRQ-40, rabbit monoclonal, 1:160 dilution, Cell Marque Corp., Rocklin, CA, USA), NeuN (clone A60, mouse monoclonal, 1:100 dilution, Millipore, Burlington, MA, USA), CD34 (clone QBEnd/10, mouse monoclonal, Ventana Medical Systems), Ki-67 (clone MIB-1, mouse monoclonal, 1:100 dilution, Dako Agilent).

Sievers P., Appay R., Schrimpf D., Stichel D., Reuss D.E., Wefers A.K., Reinhardt A., Coras R., Ruf V.C., Schmid S., de Stricker K., Boldt H.B., Kristensen B.W., Petersen J.K., Ulhøi B.P., Gardberg M., Aronica E., Hasselblatt M., Brück W., Bielle F., Mokhtari K., Lhermitte B., Wick W., Herold-Mende C., Hänggi D., Brandner S., Giangaspero F., Capper D., Rushing E., Wesseling P., Pfister S.M., Figarella-Branger D., von Deimling A., Sahm F, & Jones D.T.W. (2019). Rosette-forming glioneuronal tumors share a distinct DNA methylation profile and mutations in FGFR1, with recurrent co-mutation of PIK3CA and NF1. Acta neuropathologica, 138(3).

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