After transesterification, the FAMEs (fatty acid methyl esters (FAME)) and residual triacylglycerides (TAGs) were measured using 0.25 mm thick silica gel G-60 TLC plates (Merck, Darmstadt, Germany). The detailed method was as follows: the elution solvent was n-hexane : diethyl ether (90 : 10, v/v) and after full development to the detection of the FAME spots, the spots were visualized using an iodine vapor and spraying the plates with 10% phosphomolybdic acid (98% purity, Daejung Co., Ltd, Shi Heung, Korea) in ethanol, and then drying them in the oven at 105°C [26 ]. The mono-, di-, and triglyceride mixture (TAG STD, Supelco, Bellefonte, PA, USA) and commercial biodiesel (FAME, S Company Houston, TX, USA) were used as standards [26 ]. Additionally, the pigments that remained in the FAME fraction were also separated by TLC, using the same 0.25 mm thick silica gel G-60 plates (Merck, Darmstadt, Germany) that were developed using petroleum ether (99.5%, Daejung Co., Ltd, Shi Heung, Korea) : cyclo hexane (%, Wako, Osaka, Japan) : ethyl acetate (99%, Daejung Co., Ltd, Shi Heung, Korea) : acetone (99.5%, Daejung Co., Ltd, Shi Heung, Korea) : methanol (99.5%, Daejung Co., Ltd, Shi Heung, Korea) (60 : 16 : 10 : 10 : 4, v/v) [27 ].
G 60 tlc plates
Manufactured by Merck Group
Sourced in Germany
The G-60 TLC plates are a type of laboratory equipment used for thin-layer chromatography (TLC) analysis. TLC is a widely used analytical technique for the separation, identification, and purification of chemical compounds. The G-60 TLC plates provide a stable and uniform surface for the separation of complex mixtures. They are composed of a thin layer of silica gel, which acts as the stationary phase, and are designed to facilitate efficient chromatographic separations.
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2 protocols using g 60 tlc plates
1
Analytical Characterization of FAMEs and TAGs
2
Dansylamines Separation and Quantification
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Chromatographic separation of the investigated dansylamines was
carried out using one-dimensional silica gel G-60 TLC plates (20 ×
20 cm) from Merck, Darmstadt, Germany. 500 μL of methanol was
used to dissolve the dry layer of samples and standards. Dissolved
samples and standards (10 μL) were spotted. Chloroform, benzene,
and triethyl amine (6:4.5:1, v/v/v) were used to develop the plate. All plates were
visualized immediately after drying at room temperature, and the fingerprint
profile was photodocumented under 365 nm in a UV light source. Using
a CS-9000 dual wavelength flying spot scanning densitometer (SHIMADZU)
at a wavelength of 254 nm, the resulting spots were then marked, and
the marked areas were counted in the “Microanalysis Centre,
Faculty of Science, Cairo University, Egypt.” BA concentrations
in the examined samples were calculated by using the standard curve
for each dansylamine.
carried out using one-dimensional silica gel G-60 TLC plates (20 ×
20 cm) from Merck, Darmstadt, Germany. 500 μL of methanol was
used to dissolve the dry layer of samples and standards. Dissolved
samples and standards (10 μL) were spotted. Chloroform, benzene,
and triethyl amine (6:4.5:1, v/v/v) were used to develop the plate. All plates were
visualized immediately after drying at room temperature, and the fingerprint
profile was photodocumented under 365 nm in a UV light source. Using
a CS-9000 dual wavelength flying spot scanning densitometer (SHIMADZU)
at a wavelength of 254 nm, the resulting spots were then marked, and
the marked areas were counted in the “Microanalysis Centre,
Faculty of Science, Cairo University, Egypt.” BA concentrations
in the examined samples were calculated by using the standard curve
for each dansylamine.
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