Gradient criterion tgx gel

Manufactured by Bio-Rad

The Gradient Criterion TGX gel is a precast polyacrylamide gel used for protein electrophoresis. It features a linear gradient of acrylamide/bis-acrylamide concentrations, which allows for the separation of proteins based on their molecular weight.

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Lab products found in correlation

Clarity ecl, by Bio-Rad (2 mentions) Gradient nupage 4 12 bis tris protein gels, by Thermo Fisher Scientific (2 mentions) Fusion fx, by Vilber (1 mentions) Ecl prime kit, by GE Healthcare (1 mentions)

Close spelling variants of this product

Criterion TGX stain-free 4-15 % gradient gels 4–20% Criterion TGX gradient gels TGX Criterion gradient SDS polyacrylamide gels Criterion TGX gradient Criterion TGX gels 4–20% TGX gradient gel CriterionTM TGXTM Criterion TGX Criterion gels TGX gels

2 protocols using gradient criterion tgx gel

Protein Extraction and SDS-PAGE Analysis

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Proteins were extracted in pre-heated (95°C) 2X Laemmli sample buffer (62 mM Tris HCl pH 6.8, 3% SDS, 40% glycerol, 0,1% bromophenol blue, DTT 100mM, quantified using amido-black staining⁹⁷ (link) and 20μg of total proteins were separated by SDS-PAGE, either on 7–12% Tris-glycine gels or gradient NuPAGE 4–12% Bis-Tris Protein Gels (Thermo Fischer) or gradient Criterion TGX gel (4–15%) (BioRad). The list of antibodies are reported in the key resources table. For all western blots, immuno-luminescence was detected using the ECL Clarity (BioRad) and imaged using Fusion FX (Vilber).

Blagojevic A., Baldrich P., Schiaffini M., Lechner E., Baumberger N., Hammann P., Elmayan T., Garcia D., Vaucheret H., Meyers B.C, & Genschik P. (2024). Heat stress promotes Arabidopsis AGO1 phase separation and association with stress granule components. iScience, 27(3), 109151.

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Western Blot Protein Extraction and Analysis

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Proteins were extracted in pre-heated (95°C) 2X Laemmli sample buffer, quantified using amido-black staining (Popov et al., 1975 (link)) and 10 to 20μg of total proteins were separated by SDS-PAGE, either on 7–12% Tris-glycine gels or gradient NuPAGE 4–12% Bis-Tris Protein Gels (Thermo Fischer) or gradient Criterion TGX gel (4–15%) (BioRad). List of antibodies and their working dilution used in this work are reported in (Table S5). For all western blots, immuno-luminescence was detected using the ECL Prime kit (GE Healthcare) or ECL Clarity (BioRad) and imaged using Fusion FX (Vilbert).

Hacquard T., Clavel M., Baldrich P., Lechner E., Pérez-Salamó I., Schepetilnikov M., Derrien B., Dubois M., Hammann P., Kuhn L., Brun D., Bouteiller N., Baumberger N., Vaucheret H., Meyers B.C, & Genschik P. (2022). The Arabidopsis F-box protein FBW2 targets AGO1 for degradation to prevent spurious loading of illegitimate small RNA. Cell Reports, 39(2), 110671.

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