Biosciences lsrfortessa

Manufactured by BD

The BD Biosciences LSRFortessa is a flow cytometry instrument designed for high-performance cell analysis and sorting. It is equipped with multiple laser configurations and a range of detectors to enable comprehensive analysis of diverse cell populations.

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Lab products found in correlation

Paraformaldehyde (pfa), by BioLegend (1 mentions) Live dead fixable aqua, by Thermo Fisher Scientific (1 mentions) Rainbow beads, by BioLegend (1 mentions) Rbc lysis buffer, by Thermo Fisher Scientific (1 mentions)

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LSRFortessa (6355 citations)

2 protocols using biosciences lsrfortessa

Comprehensive PBMC Immunophenotyping

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Cryopreserved PBMCs were thawed and rested for 2 h at 37°C in R‐20 media prior to staining. Rested cells were harvested and resuspended in PBS before being transferred to a 96‐well plate for staining. PBMCs were first stained with antibodies against CXCR5 (BB515, clone RF8B2, BD Biosciences), CCR7 (APC/Cy7, clone G043H7, Biolegend) and CXCR3 (PE/Cy5, clone 1C6/CXCR3, BD Biosciences) at 37°C, followed by a second panel of antibodies at 4°C targeting CD3 (PerCP/eFluor710, clone SK7, Thermo Fisher), CD4 (PE/Dazzle594, clone RPA‐T4, Biolegend), CD8 (BV711, clone RPA‐T8, Biolegend), CD14 (BV510, clone M5E2, Biolegend), CD19 (APC, clone HIB19, Biolegend), CD25 (PE/Cy7, clone 2A3, BD Biosciences), CD38 (BV785, clone HIT2, Biolegend), CD45RA (AF700, clone HI100, Biolegend), CD56 (BV605, clone NCAM16.2, BD Biosciences), CD127 (BV650, clone A019D5, Biolegend) and PD‐1 (BV421, clone EH12.2H7, Biolegend), as well as a viability dye (Live/Dead Fixable Aqua, Thermo Fisher). Cells were fixed prior to acquisition using 2% paraformaldehyde (Biolegend). Samples were acquired on a BD Biosciences LSRFortessa calibrated using Rainbow beads (Biolegend).

Pinder C.L., Jankovic D., Fox T.A., Kirkwood A., Enfield L., Alrubayyi A., Touizer E., Ford R., Pocock R., Shin J., Ziegler J., Thomson K.J., Ardeshna K.M., Peppa D., McCoy L.E, & Morris E.C. (2023). Humoral and cellular responses to SARS‐CoV‐2 in patients with B‐cell haematological malignancies improve with successive vaccination. British Journal of Haematology, 202(6), 1091-1103.

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Circulating Tumor Cells Quantification

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Submandibular mouse blood samples were collected into EDTA-coated tubes on ice to prevent clotting. Erythrocytes were lysed and removed from blood via incubation with RBC lysis buffer (eBioscience) according to manufacturer’s protocol. Cells were fixed in 2% paraformaldehyde for 10 minutes, followed by centrifugation at 500g for 5 minutes at 4°C, and resuspended in 1% BSA/PBS. Flow cytometry (FACS) was performed using a BD Biosciences/LSR Fortessa to count RFP-positive circulating tumor cells in the blood samples. Final counts were normalized to initial primary tumor size at week zero.

Jones B.C., Kelley L.C., Loskutov Y.V., Marinak K.M., Kozyreva V.K., Smolkin M.B, & Pugacheva E.N. (2017). Dual Targeting of Mesenchymal and Amoeboid Motility Hinders Metastatic Behavior. Molecular cancer research : MCR, 15(6), 670-682.

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