Bx61vs microscope scanner

Immediately after slaughter, 10 samples of the buccal mucosa and buccal muscle were immersed in Bouin’s solution for 48 h. Afterwards, the collected tissues were dehydrated and embedded in paraffin blocks. In the next stage, the paraffin blocks were cut with a semi-automatic rotary microtome (Leica RM 2145, Leica Microsystems, Nussloch, Germany), and all paraffin sections were stained with standard hematoxylin and eosin (H&E) staining method. In the first step, the paraffin sections were deparaffinized and rehydrated. After this step, the samples were stained with hematoxylin and eosin. In the end, the sections were dehydrated and coverslipped. Subsequently, histological sections were assessed with a light microscope, and selected pictures were taken using a scanning technique by Olympus BX61VS microscope scanner (Olympus, Tokyo, Japan).

Immediately after collection, ten oviducts were fixed with Bouin’s solution (a mixture of picric acid, 40% formaldehyde and acetic acid; picric acid and acetic acid were mixed 15:1) for 48 h at RT. Subsequently, tissues were embedded in paraffin wax and cut into 3–4 μm sections with a semi-automatic rotary microtome (Leica RM 2145; Leica Microsystems GmbH, Nussloch, Germany). Oviductal sections were deparaffinized and rehydrated prior to H&E staining, and finally dehydrated. The sections were stained in hematoxylin for 20 min and in eosin for 40 min at RT. The sections were observed with a light microscope and selected images were taken with a high-resolution scanning technique using an Olympus BX61VS microscope scanner (Olympus Corporation, Tokyo, Japan). Slides were scanned at magnification, ×20. The images presented in publication consist of digitally magnified selected fragments of the scans.

Immediately after harvesting, both ITA and SV segments were fixed with Bouin’s solution for 48 h. Subsequently, the collected tissues were dehydrated and embedded in paraffin blocks and then cut into 4-µm thick sections with a semi-automatic rotary microtome (Leica RM 2145, Leica Microsystems, Nussloch, Germany). Afterwards, the blood vessels sections were stained via the routine hematoxylin and eosin (H&E) method, following the protocol of: deparaffinization and rehydration with xylene, decreasing concentrations of alcohols and water, H&E staining, and dehydration with increasing concentrations of alcohols and xylene. Histological sections were evaluated under a light microscope, with selected pictures were taken using a high-resolution scanning technique and Olympus BX61VS microscope scanner (Olympus, Tokyo, Japan).

Shortly after the collection of tissues, oviducts from 10 gilts were sectioned to separate the infundibulum, ampulla and isthmus regions. Subsequently, tissues were fixed for 48 h in Bouin’s solution, dehydrated, embedded in paraffin blocks and cut with a rotary microtome into sections of 3–4 µm. Obtained paraffin sections were stained using a hematoxylin and eosin (H + E) staining technique, following a previously described protocol [71 (link)]. Analysis of all sections was performed under a light microscope, with pictures taken using high-resolution scanning technique (Olympus BX61VS microscope scanner, Olympus, Tokyo, Japan).