Chromid cps elite agar

Manufactured by bioMérieux

Sourced in France

CHROMID® CPS® Elite agar is a selective and differential culture medium used for the isolation and identification of Candida species from clinical samples. The medium contains chromogenic and fluorogenic substrates that allow the detection and presumptive identification of Candida species based on colony color and fluorescence.

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Lab products found in correlation

Axiocam 305, by Zeiss (1 mentions) Macconkey agar, by BD (1 mentions) Matrix assisted laser desorption ionization time of flight mass spectrometry, by Bruker (1 mentions) Live dead fixable green dead cell stain, by Thermo Fisher Scientific (1 mentions) Vybrant fam caspase 3 and 7 assay kit, by Thermo Fisher Scientific (1 mentions) Mitosox red, by Thermo Fisher Scientific (1 mentions)

4 protocols using chromid cps elite agar

Phenotypic Profiling of Bacterial Variants

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@Sequential subcultures of all phenotypic variants were carried out on various agar plates (including Columbia agar + 5% sheep blood, MacConkey agar from BD, and CHROMID® CPS® Elite agar from bioMérieux) to observe whether changes in colony morphology occurred and SCVs remained stable, followed by meticulous analysis of generated phenotypic profiles.
In order to determine colony sizes, each phenotype was inoculated onto 5% sheep blood agar plates in triplicate on different days. After overnight incubation at 35 ± 1°C in ambient air, the diameters of ten colonies of each phenotype were measured and mean values were determined. Additionally, colony morphology in different phenotypes was assessed using the stereo zoom microscope Axio Zoom.V16, equipped with the objective Plan Z 1.0x/0.25 and the Axiocam 305 camera (Zeiss, Oberkochen, Germany). After Gram staining, single cells from different phenotypes were observed in transmission light by the Axio Imager.Z2m microscope with the oil immersion objective Plan-APOCHROMAT 100x/1.4 and Axiocam 305 camera (Zeiss).

Doğan E., Sydow K., Heiden S.E., Eger E., Wassilew G., Proctor R.A., Bohnert J.A., Idelevich E.A., Schaufler K, & Becker K. (2024). Klebsiella pneumoniae exhibiting a phenotypic hyper-splitting phenomenon including the formation of small colony variants. Frontiers in Cellular and Infection Microbiology, 14, 1372704.

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Screening for Carbapenem-Resistant Bacteria

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The concept of Blackburn and coworkers’ method for screening carbapenem-resistant bacteria was adopted [9 (link)], with some modifications. Briefly, fecal specimens with approximate sizes of 10 μL inoculation loop were homogenized in 500 μL sterile, double-deionized water and centrifuged at 1,500 rpm for 30 s. An aliquot of 10 μL supernatant from each sample was inoculated on chromID® CPS® Elite agar (bioMérieux, Marcy, I’Etoile, France) and a piece of polymyxin B disc (PB300, Oxoid, Basingstoke, Hampshire, England) was applied on the area of inoculation, followed by overnight incubation at 35 ^oC in ambient air. Bacterial growth around polymyxin B disc, namely inside the inhibition zone of approximately 11 mm in diameter (taking the Clinical and Laboratory Standards Institute (CLSI) guidelines’ interpretative criteria of polymyxin B for Pseudomonas aeruginosa as reference), was subjected to polymerase chain reaction (PCR) targeting mcr-1 and mcr-2 genes.

Chan W.S., Au C.H., Ho D.N., Chan T.L., Ma E.S, & Tang B.S. (2018). Prospective study on human fecal carriage of Enterobacteriaceae possessing mcr-1 and mcr-2 genes in a regional hospital in Hong Kong. BMC Infectious Diseases, 18, 81.

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Urine sediment culture protocol

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Sediment culture was performed in parallel to enrich for microorganisms, as this was previously shown to be more sensitive.^{25 (link)} Briefly, 5–15 mL of urine was centrifuged at 1400×g for 10 min. The sediment was resuspended in 400 µL of sterile phosphate buffer saline (PBS) (Life Technologies, United Kingdom). Tenfold serial dilutions were performed in PBS, and 50 µL volumes were plated onto ChromID CPS Elite agar (bioMérieux, France). Cultures were incubated aerobically at 37°C for 18–24 h. Following incubation, microbial colonies were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (Bruker Daltonics, United States).

Sathiananthamoorthy S., Florman K., Richard D., Cheng K.K., Torri V., McCaig F., Harber M, & Rohn J.L. (2023). Application of Various Techniques to Gain Insights Into the Complex Urinary Tract Microbial Communities of Renal Transplant Recipients. Transplantation Direct, 9(2), e1418.

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Bacterial Strain Preparation and Characterization

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Human tubal fluid (HTF) medium was purchased from Biocare Europe (Rome, Italy). MitoSOX™ Red, LIVE/DEAD™ Fixable Green Dead Cell Stain and Vybrant FAM Caspase 3 and 7 Assay Kit were purchased from Thermo Fisher Scientific (Waltham, MA, USA). In Situ Cell Death Detection Kit, fluorescein was obtained from Sigma Aldrich (St. Louis, MO, USA).
Seven bacterial reference strains were included in the study as follows: two isolates of E. coli (ATCC 29522 and ATCC 35218), one isolate of K. pneumoniae (ATCC 13883), one isolate of K. quasipneumoniae (ATCC 700603), one isolate of P. aeruginosa (ATCC 27853), one E. cloacae (ATCC 13047) and one K. aerogenes (ATCC 13048). An E. coli K12 strain MG1655 and its derivative (with the knockout recX gene), from the Keio collection, were also added to the study collection^{52 (link)}. Most of the selected reference strains were isolated from clinical human samples, except for K. pneumoniae ATCC 13883 and E. coli ATCC 25922 whose source is unknown (Supplemental Table S1). All bacterial strains were seeded on CHROMID® CPS® Elite agar (bioMérieux, Marcy l’Etoile, France) and incubated for 18 h at 35 ± 1 °C. Bacterial suspensions were prepared in 2 ml of sterile water and optical density was measured by DensiCHEK™ spectrophotometer (bioMérieux).

Marchiani S., Baccani I., Tamburrino L., Mattiuz G., Nicolò S., Bonaiuto C., Panico C., Vignozzi L., Antonelli A., Rossolini G.M., Torcia M, & Baldi E. (2021). Effects of common Gram-negative pathogens causing male genitourinary-tract infections on human sperm functions. Scientific Reports, 11, 19177.

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