Cells were fixed with 70% ethanol for 15 min at 4°C and stained with 50 mg/ml propidium iodide (Sigma-Aldrich) in PBS containing 0.5 mg/ml bovine pancreatic RNAase A (Sigma-Aldrich) for 1 hour at 37° C. Above 1x104 cells were analyzed in each sample using a flow cytometer Epics”Elite” (Beckman Coulter Inc., USA) equipped with an argon laser Cyonics (Uniphase, USA) and Multigraph software (Coulter, USA). Experiments were repeated four times. Then the data were analyzed using Kaluza Analysis Software (Beckman Coulter Inc., USA) .
Bovine pancreatic rnaase a
Manufactured by Merck Group
Sourced in United States
Bovine pancreatic RNAase A is a laboratory reagent used in molecular biology and biochemistry. It is an enzyme that catalyzes the hydrolysis of ribonucleic acid (RNA) molecules. This product is purified from the bovine pancreas.
Automatically generated - may contain errors
2 protocols using bovine pancreatic rnaase a
1
Cell Cycle Analysis by Flow Cytometry
2
Cell Cycle Analysis of 89SrCl2 Treated Cells
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A specified number of cells of each group were seeded into six-well culture plates. Then cells were treated with 10 µCi/ml of 89SrCl2 for 24 h. Cells (1 × 106 cells/ml) were harvested by trypsinization, washed by ice-cold PBS, and fixed in ice-cold 70% ethanol. Cells were then incubated with bovine pancreatic RNAase A (500 U/ml; Sigma-Aldrich, St. Louis, MO, USA) at 37°C for 30 min, and then 0.25% propidium iodide (PI; Sigma-Aldrich) was added to stain cells for 30 min at room temperature. Finally, cells in each group were analyzed at 488 nm by flow cytometry on a Beckman Coulter FC200.
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