Nano glo dual luciferase reporter assay system kit

Manufactured by Promega

The Nano-Glo® Dual-Luciferase® Reporter Assay System kit provides a sensitive and versatile method for quantitatively measuring the activities of two different luciferase reporter enzymes within the same sample. The kit includes reagents necessary for performing dual-reporter assays.

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Lab products found in correlation

Las af, by Leica (1 mentions) Tcs sp5 germany, by Leica (1 mentions)

Close spelling variants of this product

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2 protocols using nano glo dual luciferase reporter assay system kit

LDHA Promoter-Driven Luciferase Assay

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The DNA sequence of the LDHA promoter, spanning 2000 bp upstream, was inserted into the pGL-Basic firefly luciferase plasmid to create the LDHA promoter-reporter gene. The pRL-TK Renilla luciferase plasmid was used as the endogenous reference reporter gene. The experiment was conducted using the Nano-Glo® Dual-Luciferase® Reporter Assay System kit (E1910) from Promega, following the instructions outlined in the assay kit manual.

Chen S., Xie D.F., Li S., Luo J., Han Y., Guo H., Gao S., Huang X., Guan H., Huang R, & Zhou P.K. (2024). TAB182 regulates glycolytic metabolism by controlling LDHA transcription to impact tumor radiosensitivity. Cell Death & Disease, 15(3), 209.

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Dual-Luciferase Reporting in S. miltiorrhiza Protoplasts

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Use a confocal laser scanning microscope to observe protoplasts (Leica TCS SP5 Germany) and visualized them by a Leica Microsystem LAS AF. GFP was excited at 488nm wavelengths. All uorescence observation experiments were repeated at least three times independently.
Dual-luciferase and edit e ciency evaluation in S. miltiorrhiza protoplast Protoplast samples were collected into 2mL tubes after 16-24 hours of incubation in the dark. fLUC and NanoLUC activities were analyzed by the Nano-Glo® Dual-Luciferase® Reporter Assay System Kit (Promega). Assume the luminescence values of wild-type untransformed protoplasts serve as a reference for relative uorescence values. S. miltiorrhiza protoplast editing e ciency was quanti ed by calculating the change in the NanoLUC to fLUC ratio.

Shao J., Peng B., Zhang Y., Yan X., Yao X., Hu X., Li L., Fu X., Zheng H, & Tang K. (2024). A high-efficient protoplast transient system for screening gene editing elements in Salvia miltiorrhiza. Plant cell reports, 43(2).

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