The BCA assay quantified proteins, and loading buffer 5X was added to the proteins, which were incubated for 5 min at 95°C. Then, proteins were loaded on an SDS-PAGE polyacrylamide gel, transferred to Immobilon-P PVDF membrane (Millipore, Shanghai, China), probed with the appropriate primary antibodies: COX-1 (1:5000), COX-2 (1:4000), and PTGFR (1:2000) were obtained from Abcam (Cambridge, UK), and TBXA2R (1:4000), GAPDH (1:10000), and detected by chemiluminescence (ECL, Thermo Scientific). Images were then acquired with Image-Lab software (Bio-Rad, Hercules, CA, USA). Image analysis of western blots was performed with Image-Lab analyzer software.
Ptgfr
Manufactured by Abcam
Sourced in United Kingdom
PTGFR is a product offered by Abcam that serves as a receptor for prostaglandin F2-alpha. It is a member of the G protein-coupled receptor family and is involved in various physiological processes.
Automatically generated - may contain errors
Lab products found in correlation
Image lab software, by Bio-Rad (1 mentions)
Enhanced chemi luminescence (ecl), by Thermo Fisher Scientific (1 mentions)
Gapdh, by Thermo Fisher Scientific (1 mentions)
Cox 2, by Abcam (1 mentions)
Immobilon p pvdf membrane, by Merck Group (1 mentions)
Tbxa2r, by Thermo Fisher Scientific (1 mentions)
Other antibodies, by Proteintech (1 mentions)
2 protocols using ptgfr
1
Quantification and Visualization of Protein Levels
2
Oxidative Stress in Trophoblast Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HTR-8/SVneo cells (provided by Dr. Charles Graham, Queen's University of Canada) at the logarithmic growth stage were cultured in a 37°C, 5% CO 2 incubator plated on a six-well culture plate at a density of 1 × 10 5 cells per well. After 24 hours, H 2 O 2 (Sigma-Aldrich, USA) was added at concentrations of 300 µmol/L and 3hours to the culture to establish an oxidative stress model of HTR-8/SVneo cells (Model). Cells in the experimental group were incubated with gradient diluted BHD water extracts (1 mg/mL, 5 mg/mL, 10 mg/mL, 20 mg/mL) for 48 h, and cells without any treatment were used as the control group (Control). The morphology of the cells was observed under an inverted microscope.
PTGFR was obtained from Abcam (Cambridge, UK), and other antibodies were obtained from Proteintech (Chicago, USA). PTGFR (ab126709, 1:2000), AGTR1(25343-1-AP, 1:500), OXTR (23045-1-AP, 1:1000), GAPDH (10494-1-AP, 1:5000).
PTGFR was obtained from Abcam (Cambridge, UK), and other antibodies were obtained from Proteintech (Chicago, USA). PTGFR (ab126709, 1:2000), AGTR1(25343-1-AP, 1:500), OXTR (23045-1-AP, 1:1000), GAPDH (10494-1-AP, 1:5000).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!