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Procartaplex mix match human plex

Manufactured by Thermo Fisher Scientific
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The ProcartaPlex Mix&Match Human plex is a multiplex assay system used for the simultaneous measurement of multiple analytes in a single sample. It provides a flexible platform for analyzing a customized panel of protein targets relevant to your research.

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Lab products found in correlation

Cardiophase hscrp, by Siemens (2 mentions) Penicillin, by BioConcept (1 mentions) Streptomycin, by BioConcept (1 mentions) Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

ProcartaPlex Mix&-Match Human 13-plex Human ProcartaPlex™ Mix&Match 4-plex

4 protocols using procartaplex mix match human plex

1

Cytokine Profiling of Activated PBMCs

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PBMCs (2 × 105 cells) were stimulated for 24 h in complete medium with ESAT-6 and CFP-10 peptide pools (1 μg/ml) or with SEB (250 ng/ml) or were left unstimulated (negative control). At the end of the stimulation period, culture supernatants were collected and levels of TNF-α, IFN-γ, IL-2, IL-4, IL-5, IL-13, IL-10, IL-17A, IL-17F, IL-21, and IL-22 were assessed cells by multiplex bead assay (ProcartaPlex Mix&Match Human plex; eBioscience).
Amelio P., Portevin D., Hella J., Reither K., Kamwela L., Lweno O., Tumbo A., Geoffrey L., Ohmiti K., Ding S., Pantaleo G., Daubenberger C, & Perreau M. (2019). HIV Infection Functionally Impairs Mycobacterium tuberculosis-Specific CD4 and CD8 T-Cell Responses. Journal of Virology, 93(5), e01728-18.
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2

Cytokine profiling of PBMC stimulation

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PBMCs (2x105 cells) were stimulated for 24 hours in complete media (RPMI (Invitrogen), 10% fetal calf serum (FCS; Invitrogen), 100 µg/ml penicillin, 100 unit/ml streptomycin (BioConcept)) with ESAT-6 and CFP-10 peptide pools (1 µg/ml) or with Staphyloccocus enterotoxin B (SEB; 250 ng/mL) or left unstimulated (negative control). At the end of the stimulation period, culture supernatants were collected and levels of TNF-α, IFN-γ, IL-2, IL-4, IL-5, IL-13, IL-10, IL-17A and IL-17F were assessed cells by luminex assay (ProcartaPlex Mix&Match Human plex, eBioscience).
Amelio P., Portevin D., Reither K., Mhimbira F., Mpina M., Tumbo A., Nickel B., Marti H., Knopp S., Ding S., Penn-Nicholson A., Darboe F., Ohmiti K., Scriba T.J., Pantaleo G., Daubenberger C, & Perreau M. (2017). Mixed Th1 and Th2 Mycobacterium tuberculosis-specific CD4 T cell responses in patients with active pulmonary tuberculosis from Tanzania. PLoS Neglected Tropical Diseases, 11(7), e0005817.
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3

Cytokine and CRP Measurement Protocol

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Serum levels of IL-1α, IL-6, TNF-α, IL-12p70, IL-23, IL-10, IFN-α2, IFN-β, IFN-ω and IFN-γ was assessed by luminex assay (ProcartaPlex Mix&Match Human plex, eBioscience) and CRP was assessed by nephelemetry (CardioPhasehsCRP, Siemens Healthcare Diagnostics Products GmbH) as previously described [96 (link)].
Amelio P., Portevin D., Reither K., Mhimbira F., Mpina M., Tumbo A., Nickel B., Marti H., Knopp S., Ding S., Penn-Nicholson A., Darboe F., Ohmiti K., Scriba T.J., Pantaleo G., Daubenberger C, & Perreau M. (2017). Mixed Th1 and Th2 Mycobacterium tuberculosis-specific CD4 T cell responses in patients with active pulmonary tuberculosis from Tanzania. PLoS Neglected Tropical Diseases, 11(7), e0005817.
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4

Multiplex Cytokine Profiling Protocol

Cited 1 time
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Serum levels of IL-1α, IL-6, IL-23, IL-23, and IP-10 were assessed by Luminex assay (ProcartaPlex Mix&Match Human plex; eBioscience), and CRP was assessed by nephelemetry (CardioPhasehsCRP; Siemens Healthcare Diagnostics Products GmbH) as previously described (27 (link)).
Amelio P., Portevin D., Hella J., Reither K., Kamwela L., Lweno O., Tumbo A., Geoffrey L., Ohmiti K., Ding S., Pantaleo G., Daubenberger C, & Perreau M. (2019). HIV Infection Functionally Impairs Mycobacterium tuberculosis-Specific CD4 and CD8 T-Cell Responses. Journal of Virology, 93(5), e01728-18.
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