Cells were lysed in RIPA lysis buffer (50 mM Tris-HCl [pH 7.5], 150 mM NaCl, 1% NP-40, 0.1% SDS, and 1% sodium deoxycholate) supplemented with protease inhibitors (Roche), sonicated, and centrifuged at 13,200 rpm for 15 min. Protein concentrations were measured using the Dc-Protein Kit (Bio-Rad). Equal amounts of protein were separated on SDS-PAGE, blotted, probed for target proteins, and detected using ECL (Perkin Elmer). The primary antibodies used for detection were UBF (F-9; Santa Cruz Biotechnology), RRN3 (ab112052; Abcam), TAF1C (ab134394; Abcam), POLR1A/RPA194 (C-1; Santa Cruz Biotechnology), POLR1B/RPA135 (H-15; Santa Cruz Biotechnology), RPA43 (HPA022416; Sigma-Aldrich), α-tubulin (10D8; Santa Cruz Biotechnology), lamin A/C (H-110; Santa Cruz Biotechnology), and GAPDH (14C10; Cell Signaling Technology). Horseradish peroxidase (HRP)-conjugated secondary antibodies were from DAKO or Santa Cruz Biotechnology. Protein densitometry analysis was conducted using ImageJ software, and the mean value normalized with loading control was used as final protein band quantification.
Polr1b rpa135 h 15
Manufactured by Santa Cruz Biotechnology
POLR1B/RPA135 (H-15) is an antibody that targets the POLR1B protein, also known as RPA135. POLR1B is a subunit of RNA polymerase I, which is responsible for the transcription of ribosomal RNA genes. The antibody can be used for applications such as Western blotting and immunoprecipitation to detect and study the POLR1B protein.
Automatically generated - may contain errors
Lab products found in correlation
Dc protein kit, by Bio-Rad (2 mentions)
Ubf f 9, by Santa Cruz Biotechnology (2 mentions)
Polr1a rpa194 c 1, by Santa Cruz Biotechnology (2 mentions)
Taf1c, by Abcam (2 mentions)
Rpa43 hpa022416, by Merck Group (2 mentions)
Lamin a c h 110, by Santa Cruz Biotechnology (2 mentions)
α tubulin, by Santa Cruz Biotechnology (2 mentions)
Gapdh, by Cell Signaling Technology (2 mentions)
Protease inhibitor, by Roche (2 mentions)
Enhanced chemi luminescence (ecl), by PerkinElmer (2 mentions)
2 protocols using polr1b rpa135 h 15
1
Quantitative Western Blot Analysis
2
Quantitative Western Blot Analysis
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Cells were lysed in RIPA lysis buffer (50 mM Tris-HCl [pH 7.5], 150 mM NaCl, 1% NP-40, 0.1% SDS, and 1% sodium deoxycholate) supplemented with protease inhibitors (Roche), sonicated, and centrifuged at 13,200 rpm for 15 min. Protein concentrations were measured using the Dc-Protein Kit (Bio-Rad). Equal amounts of protein were separated on SDS-PAGE, blotted, probed for target proteins, and detected using ECL (Perkin Elmer). The primary antibodies used for detection were UBF (F-9; Santa Cruz Biotechnology), RRN3 (ab112052; Abcam), TAF1C (ab134394; Abcam), POLR1A/RPA194 (C-1; Santa Cruz Biotechnology), POLR1B/RPA135 (H-15; Santa Cruz Biotechnology), RPA43 (HPA022416; Sigma-Aldrich), α-tubulin (10D8; Santa Cruz Biotechnology), lamin A/C (H-110; Santa Cruz Biotechnology), and GAPDH (14C10; Cell Signaling Technology). Horseradish peroxidase (HRP)-conjugated secondary antibodies were from DAKO or Santa Cruz Biotechnology. Protein densitometry analysis was conducted using ImageJ software, and the mean value normalized with loading control was used as final protein band quantification.
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