Shimadzu—UV 1800 double beam UV–Visible spectrophotometer (Japan) and quartz cells (1 cm) at a range of 200.0–400.0 nm was used for measuring the absorbance. Spectral manipulations were carried out by Shimadzu UV-Probe 2.32 system software.
Uv 1800 double beam uv visible spectrophotometer
Manufactured by Shimadzu
Sourced in Japan
The UV 1800 is a double beam UV–Visible spectrophotometer manufactured by Shimadzu. It is designed to measure the absorption or transmission of light in the ultraviolet and visible regions of the electromagnetic spectrum.
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Lab products found in correlation
4 protocols using uv 1800 double beam uv visible spectrophotometer
1
UV-Vis Spectrophotometric Absorbance Analysis
2
Characterization of Organic Compounds
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A Shimadzu UV-1800 double-beam UV–visible spectrophotometer with 1-cm quartz cells was used. A Perkin-Elmer FT-IR spectrophotometer with KBr pellet was used for recording the IR spectrum (Akron, OH, USA). The NMR spectra were obtained using a Bruker 700 MHz Nuclear Magnetic Spectrometer (Billerica, MA, USA). 1H-NMR spectra were run at 700 MHz and 13C spectra were run at 175 MHz in DMSO-d6. Melting points were measured with a Stuart apparatus (Bibby Scientific Limited, Staffordshire, UK).
3
Insect Enzyme Extraction and Quantification
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Frozen insects were ground in liquid nitrogen with a mortar and pestle. Crude enzyme extract for assay was prepared by the method of Hwang et al22
Whole insect was homogenized in three volume s of cold 100 mM sodium acetate, pH 5.5 containing 1 mM EDTA. The homogenate was centrifuged at 10,000 g for 15 minutes at 4°C. The supernatant was used for various assays. Protein concentrations were determined using Bio-Rad protein assay kit (Bio-Rad, Hercules, CA, USA,) based on the method of Bradford23 (link)
with BSA as a standard. All spectrophotometric assays were monitored using Shimadzu UV 1800 double beam UV–visible spectrophotometer.
Whole insect was homogenized in three volume s of cold 100 mM sodium acetate, pH 5.5 containing 1 mM EDTA. The homogenate was centrifuged at 10,000 g for 15 minutes at 4°C. The supernatant was used for various assays. Protein concentrations were determined using Bio-Rad protein assay kit (Bio-Rad, Hercules, CA, USA,) based on the method of Bradford23 (link)
with BSA as a standard. All spectrophotometric assays were monitored using Shimadzu UV 1800 double beam UV–visible spectrophotometer.
4
UV/Vis Spectrophotometric Analysis Protocol
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Shimadzu UV1800 double beam UV/Visible spectrophotometer (Japan) with 1 cm quartz cells. Matlab® (8.3.0.532) R2014a software (The Mathworks, Natick, USA) with PLS toolbox 2.1 was used for the mean centering spectrophotometric method calculation.
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