Tristar lb941 vti microplate reader

We used an HSV-1 IgG ELISA Kit (Phoenix Pharmaceuticals, Inc.). The anti-HSV-1 antibody titer was measured according to the attached protocol. A TriStar LB941-vTi Microplate Reader (Berthold Technologies) was used to measure optical density (450 nm) after the color reaction. Following the attached protocol, the anti-HSV-1 antibody titer was expressed as an HSV-1 IgG index by comparing the patient sample optical density and the cut-off calibrator optical density. A sample was defined as being positive for anti-HSV-1 IgG antibody if the HSV-1 IgG Index was above 1.0.

Blood samples were centrifuged, and sera were stored frozen at −80 °C until assay. We used the Human IL-1 beta/IL-1F2 Quantikine HS ELISA Kit (HSLB00D, R & D Systems, Minneapolis, MN, USA) and Human IL-6 Quantikine ELISA Kit HS (HS600C, R & D Systems, Minneapolis, MN, USA) for measurements of IL-1β and IL-6 in sera. The sensitivity and range of these assay kits were 0.063 pg/mL and 0.1–8 pg/mL, and 0.09 pg/mL and 0.16–10 pg/mL, respectively. IL-1β and IL-6 in serum were measured in duplicate according to the attached protocols. A TriStar LB941-vTi Microplate Reader (Berthold Technologies, Bad Wildbad, Germany) was used to measure optical density (450 nm, reference 560 nm). The standard curves were obtained through cubic regression using the included highly purified E. coli-expressed recombinant human IL-1β and IL-6.