Agilent 1260 infinity 2 lc

Manufactured by Agilent Technologies

Sourced in United States

The Agilent 1260 Infinity II LC is a liquid chromatography system designed for high-performance liquid chromatography (HPLC) and ultra-high performance liquid chromatography (UHPLC) applications. It provides reliable and efficient separation of complex samples, offering consistent performance and precise data acquisition.

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Lab products found in correlation

Lcq fleet mass spectrometer, by Thermo Fisher Scientific (1 mentions) 3130 genetic analyzer, by Thermo Fisher Scientific (1 mentions) J 820 spectropolarimeter, by Shimadzu (1 mentions) Ftir 8400s spectrophotometer, by Shimadzu (1 mentions) Silica gel 60 f254, by Merck Group (1 mentions) Rp 18 f254, by Merck Group (1 mentions) U 2900 spectrophotometer, by Hitachi (1 mentions) Jnm ecz400s spectrometer, by JEOL (1 mentions) Xterra rp18 column, by Waters Corporation (1 mentions) Proteome discoverer software, by Thermo Fisher Scientific (1 mentions) Chitosan, by Agilent Technologies (1 mentions)

Close spelling variants of this product

Agilent 1260 Infinity II LC system Agilent 1260 Infinity II Binary LC system Agilent 1260 Infinity LC 1260 Infinity II LC Agilent 1260 Infinity II 1260 Infinity LC Agilent 1260 Infinity Agilent 1260 LC 1260 Infinity II Agilent 1260 II

5 protocols using agilent 1260 infinity 2 lc

Purification of Tripeptoid Compounds

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Crude tripeptoid O was purified by column chromatography using a Hypersep Si column (500 mg/3 mL) using 5% methanol in dichloromethane, then 10% methanol in dichloromethane to effect complete elution from the column. The remaining tripeptoids were purified by reverse‐phase high performance liquid chromatography (RP‐HPLC) on an Agilent Technologies 1260 Infinity II system equipped with a Polaris C18‐A column (250 × 10.0 mm, 5 μm) using a 10 minute linear gradient of 30%‐90% methanol (solvent B) in 0.1% aqueous TFA (solvent A) at a flow rate of 4.7 mL/min. Peaks eluted were detected by absorbance at 220 nm. All data were visualized with OpenLab CDS software.
Peptoids were identified by electrospray mass spectrometry in positive‐ion mode using a Thermo LCQ Fleet mass spectrometer. High‐resolution mass spectral data were collected for 10 representative tripeptoids using an Agilent 1260 Infinity II LC with 6230 time of flight mass spectrometer (TOF MS) detector (electrospray ionization, positive ion mode) and were within 5 ppm of expected values. These data are tabulated in the Supporting Information, Table S1. Purified peptoids were lyophilized to white powders.

Jimenez C.J., Tan J., Dowell K.M., Gadbois G.E., Read C.A., Burgess N., Cervantes J.E., Chan S., Jandaur A., Karanik T., Lee J.J., Ley M.C., McGeehan M., McMonigal A., Palazzo K.L., Parker S.A., Payman A., Soria M., Verheyden L., Vo V.T., Yin J., Calkins A.L., Fuller A.A, & Stokes G.Y. (2019). Peptoids advance multidisciplinary research and undergraduate education in parallel: Sequence effects on conformation and lipid interactions. Biopolymers, 110(4), e23256.

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Comprehensive Analytical Characterization of Compounds

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Optical rotation values were recorded on a JASCO P-2200 polarimeter, while the UV spectra were obtained using a Hitachi U-2900 spectrophotometer. The ECD spectra were acquired on a JASCO J-820 spectropolarimeter and the IR spectra were recorded on a Shimadzu FTIR-8400S spectrophotometer. NMR spectra were acquired on a JEOL JNM-ECZ 400S spectrometer with tetramethylsilane as the internal standard. ESIMS data were obtained using an Agilent 6230B TOF LC/MS system equipped with an electrospray ion source (Agilent Technologies, CA, USA) and an Agilent 1260 infinity II LC (Agilent Technologies). DNA sequencing was performed using an Applied Biosystems 3130 genetic analyzer. Silica gel AP-300 (Toyota Kako) was employed for column chromatography (CC). Silica gel 60 F₂₅₄ and RP-18 F_254S (both Merck) were used for TLC.

Nakashima K.I., Tomida J., Tsuboi T., Kawamura Y, & Inoue M. (2020). Muyocopronones A and B: azaphilones from the endophytic fungus Muyocopron laterale. Beilstein Journal of Organic Chemistry, 16, 2100-2107.

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Melanin Pathway Substrate and Product Characterization

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The substrates and products in the melanin pathway were identified using RP-HPLC on an Agilent 1260 Infinity II LC (Agilent, Santa Clara, CA, USA). A 2 µL sample was injected at a 1.0 mL/min flow rate on a Poroshell 120 Phenyl-Hexyl, 4.6 × 100 mm, 4 µm column (Agilent, Santa Clara, CA, USA) at 30 °C with a wavelength set for detection at 280 nm. The chromatograms were analyzed using the OpenLAB CDS 2.3.53 (Agilent, Santa Clara, CA, USA). The standard of L-DOPA was prepared in 10 mM of NaPO₄ (pH 7.4) with a final concentration of 1.5 mM. The standard of DHICA was prepared in water with a final concentration of 1.5 mM. The native dopachrome was isolated from the magnetic beads with a 10 min reaction time and stored at −80 °C until each run. The samples were run with an isocratic mobile phase of 90% 10 mM NaPO₄ (pH 6.0) and 10% methanol. The samples were run in triplicates, and the retention times were averaged.

Osuna I., Dolinska M.B, & Sergeev Y.V. (2022). In Vitro Reconstitution of the Melanin Pathway’s Catalytic Activities Using Tyrosinase Nanoparticles. International Journal of Molecular Sciences, 24(1), 639.

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Peptide Analysis in Uterine Fluid

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Peptides were separated via high-performance LC (Agilent 1260 Infinity II LC; Agilent Technologies, Inc.) from uterine fluid. Samples were separated on a XTerra RP18 column (4.6x50 mm; 5 µm; Waters Corporation) at 35˚C. Mobile phases A and B were comprised of 1,000:1 (v/v) methanol/formic acid and 1,000:1 (v/v) water/formic acid, respectively. The flow rate was 0.4 ml/min. The injection volume was 3 µl and the total running time was 2 min.
Peptides were identified via MS/MS. Multiple reaction monitoring was performed with an Agilent 6460 triple quadrupole MS/MS fitted with the Agilent Jet Stream Electrospray Ionization probe (Agilent Technologies, Inc.) in the positive ion mode. Data were analyzed using the full scan mass spectra (300-1800 M/z). The mass spectrometry ion source was a nano-ESI ion source, so without parameters such as nitrogen gas temperature, nebuliser pressure and flow rate. Experiments were performed in triplicates. Finally, the mass spectrometry data were analyzed using the Proteome Discoverer software (version 1.4.0.288; Thermo Fisher Scientific, Inc.). DEPs were screened using P<0.05 and Fold Change (FC)=2 as criteria. Protein name, gene ID, molecular weight and other information were obtained using the UniProt knowledgebase (http://www.uniprot.org/).

Zhao X., Si L., Niu L., Wei M., Wang F., Liu X., Chen Z., Qiao Y., Cheng L, & Yang S. (2022). Effects of RFRP‑3 on an ovariectomized estrogen‑primed rat model and HEC‑1A human endometrial carcinoma cells. Experimental and Therapeutic Medicine, 25(2), 76.

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Characterization of Chitosan from Alaskan Snow Crabs

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Chitosan purchased from Nantong Xingcheng Biological Products Co., Ltd., Nantong, China, derived from Alaskan snow crabs, deacetylation degree: 92.7 ± 1.5%, viscosity: 50–60 cP. Molecular weight and molecular weight distribution: Mw: (2.5 ± 0.1) × 10⁵, Mn: (2.09 ± 0.04) × 10⁵, Mw/Mn = 1.21 ± 0.03.
The viscosity was determined by using an NDJ-79 rotary viscometer (Shanghai Changji Geological Instrument Co., Ltd., Nantong, China), Chitosan was dissolved in 1% acetic acid solution to a concentration of 10 mg/mL solution, and the viscosity was measured using an NDJ-79 rotary viscometer at 25 ± 2 °C.
Molecular weight and molecular weight distribution was determined by Agilent 1260 Infinity II LC (Agilent Technologies, lnc. Santa Clara, CA, USA) 3.0 g was completely dissolved in 1% acetic acid and detected by molecular exclusion chromatography.
Deacetylation level was determined by acid-base titration [19 (link),20 (link),21 (link)]. Briefly, 0.2 g of Chitosan was dissolved in 30 mL of HCl titer (0.1 mol/L). Methyl orange-aniline blue were added as an indicator, and NaOH titer (0.1 mol/L) until the neuter pH. The calculation formula is as follows:

Jiang Y., Tang X., Li T., Ling J., Ge Y, & Yang Y. (2023). Chitosan Lactate Particles for Non-Compression Hemostasis on Hepatic Resection. Polymers, 15(3), 656.

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