Acetonitrile lc ms grade

The triglyceride trimyristin (Dynasan^® 114) was donated by IOI Oleo, Witten, Germany and the surfactant poloxamer 407 (Kolliphor^® P127) by BASF AG, Ludwigshafen, Germany. Sodium alginate (Manugel^® GMB) was a kind gift from FMC International, Wallingstown, Ireland. As estimated by the supplier, the molecular weight was ~124 kDa, the content of guluronic acid (G) was 60–70% and that of mannuronic acid (M) was 30–40%. Tetrahydrofuran (HPLC grade), acetonitrile (HPLC grade), and the drugs fenofibrate and retinyl acetate were obtained from Sigma-Aldrich, Steinheim, Germany. Cannabidiol was purchased from TCH-Pharma, Frankfurt, Germany. Lumefantrine was purchased from Acros Organics, Geel, Belgium. Orlistat was donated by Formosa Laboratories Inc., Taoyuan, Taiwan. Refined rapeseed oil, sodium hydroxide, sodium azide, anhydrous glycerol, calcium chloride, acetonitrile (LC-MS grade), and tetrahydrofuran (Ultra LC-MS grade) were obtained from Carl Roth, Karlsruhe, Germany. All materials were used as received. Purified water was prepared by deionization and filtration (EASYpureTM LF, Barnstead, Dubuque, IA, USA) or was of bidistilled quality.

Acetonitrile (LC-MS grade), ammonium formate (≥95%), chloroform (HPLC grade), isopropanol, methanol (both LC-MS grade) and sodium hydroxide (≥99%) were purchased from Carl Roth (Karlsruhe, Germany). (1H,1H,2H-perfluoroethoxy)phosphazene was obtained from Santa Cruz Biotechnology (Dallas, TX, USA). Ultrapure water (resistance ≥ 18 MΩ) was obtained by treating demineralized water in a Direct-Q 3 UV-R system (Merck Millipore, Darmstadt, Germany).

Pyridoxine hydrochloride (≥ 99%) was obtained from Alfa Aesar (Bellefonte, PA, USA) and pyridoxal hydrochloride (≥ 99.5%) from Alexis Biochemicals (Lausen, Switzerland). Pyridoxamine dihydrochloride (≥ 98%), pyridoxamine-5′-phosphate (≥ 98%), and pyridoxal-5′-phosphate monohydrate (≥ 97%) were purchased from Sigma-Aldrich (Bellefonte, PA, USA). 5′-β-Pyridoxine glycoside and the stable isotopically labeled standards [¹³C₃]-PN, [¹³C₃]-PL, and [¹³C₆]-PNG were prepared in our laboratory, chromatographically purified, and characterized by NMR spectroscopy as published [48 (link), 49 (link)]. Concentrations were determined by qNMR.
Formic acid (≥ 98%) was obtained from Sigma-Aldrich (Bellefonte, PA, USA), hydrochloric acid (32%) from VWR International GmbH (Darmstadt, Germany), and starch (high purity, order number: 101252) from Merck KGaA (Darmstadt, Germany). Acetonitrile (analytical grade), methanol (analytical grade), water (analytical and LC-MS grade), and isopropanol (LC-MS grade) were received from VWR International GmbH (Ismaning, Germany). Acetonitrile (LC-MS grade) was obtained from Carl Roth GmbH & Co., KG (Karlsruhe, Germany). Methanol (LC-MS grade) was purchased from Honeywell^™ Riedel-de Häen^™ (Seelze, Germany).