Whatman no 2 filter paper

The aqueous extract of 9-year-old SC was prepared based on the method described by Zackrisson and Nilsson (1992) [45 (link)]. Briefly, 50 g of corm powder was dissolved in 1 L of distilled water (5% solution) and shaken at 100 rpm for 48 h. The solution was filtered through a Whatman filter paper (No. 2) and diluted to 75%, 50%, and 25% (as stock solution). In order to remove allelopathic interactions, 2 g of powdered activated carbon or zeolite was added to 100 mL of SC aqueous extract and shaken at 100 rpm for 12 h.
The stock solutions of saffron and non-SFS were prepared in 1 L of distilled water. In order to evaluate the effect of activated carbon and zeolite on the allelopathic activity of the extracts, 2 g of activated carbon or zeolite was added to 100 mL of saffron and non-SFS (10% solution) aqueous extract of SFS. Then, each extract (through a Whatman No. 2 filter paper) and 50 lettuce seeds were placed into Petri dishes. Distilled water was used as a control. The procedure was conducted as a factorial experiment in a completely randomized design with five replications.

The TTR sample in Fig. 3A was imaged using a Joel 1011 microscope operating at 80 kV. Samples were deposited on fresh continuous carbon films prepared from copper rhodium grids (Electron Microscopy Sciences). Grids were charged using a glow discharger for 15 seconds at 3 mA negative discharge before adding samples. Fibril solutions of 1 mg/mL were adsorbed to grids for 2 minutes before rinsing with 10 μL ddH20 for 10 seconds. Samples were then blotted using No. 2 Whatman Filter paper and stained with freshly filtered 2% uranyl acetate for 15 seconds.
Supplementary Figure S3’s TTR samples (0.2 mg/mL WT TTR in 140 mM NH₄CH₃COO, pH 4.5) were incubated with and without 70 nM misTTR antibody for 72 hours at 37 °C and 600RPM agitation. Samples were imaged using a Joel 1200 microscope operating at 80 kV at 1 hr, 24 hr, and 48 hr time points. Samples were deposited on carbon films prepared from copper rhodium grids (Electron Microscopy Sciences). Grids were charged using a glow discharger for 4–5 seconds at 3 mA prior to sample deposition. Samples were allowed 60 seconds for binding prior to wicking off with No. 2 Whatman Filter paper and then stained with freshly filtered 2% uranyl acetate for 10 seconds.

The E. cava specimens used in this study were collected from Aewol-eup, Jeju Island, Republic of Korea. Fresh E. cava was washed with water and dried at 65°C. Then, the dry seaweed was immersed in 80% (v/v) aqueous ethanol for 24 h to prepare the ECE, while the ECPE was prepared by extracting dry E. cava at 60°C for 6 h with 10 times the 70% (v/v) aqueous ethanol. The ECE and ECPE were filtered through Whatman No. 2 filter paper (Whatman International Ltd., UK), concentrated, and lyophilized.