Pmxs stat3c

Manufactured by Addgene

Sourced in United States

The PMXs-STAT3C is a lentiviral vector that expresses a constitutively active form of the STAT3 transcription factor. STAT3 is a key regulator of cellular processes such as cell growth, survival, and differentiation. The PMXs-STAT3C vector can be used for the overexpression of active STAT3 in target cells.

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Lab products found in correlation

Rpmi 1640, by Thermo Fisher Scientific (2 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Sodium dodecyl sulfate (sds), by Merck Group (2 mentions) Sodium chloride (nacl), by Merck Group (2 mentions) Glycine, by Merck Group (2 mentions) Pgem t easy vector, by Promega (1 mentions) Pbabe puro vector, by Addgene (1 mentions) Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions) Annexin 5, by BD (1 mentions) Anti parp, by Santa Cruz Biotechnology (1 mentions) Anti cyclin d1, by Cell Signaling Technology (1 mentions) Anti bcl xl, by Santa Cruz Biotechnology (1 mentions) Anti bcl 2, by Santa Cruz Biotechnology (1 mentions) Anti cox 2, by Santa Cruz Biotechnology (1 mentions) Anti iap 1, by Santa Cruz Biotechnology (1 mentions) Tris base, by Merck Group (1 mentions) Anti β actin, by Santa Cruz Biotechnology (1 mentions)

4 protocols using pmxs stat3c

Expression Vectors for STAT3, BCL-2, and BCL-xL

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The open reading frame (ORF) of the dominant-active STAT3 mutant (STAT3 (A661C/N663C); STAT3-CA) and BCL-2 were PCR-amplified using the plasmids pMXs-STAT3-C (plasmid #13373) and 3336 pcDNA3 Bcl-2 (plasmid #8768) deposited in Addgene (Cambridge, MA, USA) as the template, respectively. BCL-xL ORF was PCR-amplified from the cDNA pool of HCT 116, a human colorectal carcinoma cell line. All PCR-amplified ORFs were first TA-cloned into the pGEM-Teasy vector (Promega; Madison, WI, USA) for sequence validation. The BCL-2 ORF was later subcloned to the pBabe.puro vector (Plasmid #1764) (Addgene; Cambridge, MA, USA) at the EcoRI restriction site and orientation confirmed, whereas the ORFs of STAT3-CA and BCL-xL were directionally subcloned to the pBabe-HA vector, a pBabe.puro vector engineered to encode an in-frame N-terminal hemagglutinin (HA) epitope. The accession-numbers of STAT3, BCL-2 and BCL-xL mRNA are NM_139276, NM_000633, and NM_138578, respectively.

Cheng Y.P., Li S., Chuang W.L., Li C.H., Chen G.J., Chang C.C., Or C.H., Lin P.Y, & Chang C.C. (2019). Blockade of STAT3 Signaling Contributes to Anticancer Effect of 5-Acetyloxy-6,7,8,4′-Tetra-Methoxyflavone, a Tangeretin Derivative, on Human Glioblastoma Multiforme Cells. International Journal of Molecular Sciences, 20(13), 3366.

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Ginkgolic Acid Inhibits STAT3 Signaling

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Ginkgolic acid C 17:1 (GAC 17:1) (95% purity) and ginkgolic acid C 15:1 (GAC 15:1) (98% purity), anti-STAT3, anti-SHP-1, anti-PTEN, anti-Bcl-2, anti-Bcl-xL, anti-survivin, anti-IAP-1, anti-COX-2, anti-MMP-9, anti-MMP-2, anti-caspase-3, anti-PARP, anti-β-actin, and horseradish peroxidase (HRP)-conjugated secondary antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Tris base, glycine, NaCl, sodium dodecylsulfate (SDS), and bovine serum albumin (BSA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). RPMI 1640, MEM, and fetal bovine serum (FBS) were obtained from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Annexin V was obtained from BD Biosciences (Palo Alto, CA, USA). Anti-p-STAT3 (Tyr705), anti-p-JAK1 (Tyr1022/1023), anti-JAK1, anti-p-src (Tyr416), anti-src, anti-cyclin D1, and anti-cleaved caspase-3 were purchased from Cell Signaling Technology (Beverly, MA, USA). pMXs-gw (#18656) and pMXs-STAT3C (#13373) were obtained from Addgene (Cambridge, MA, USA).

Baek S.H., Lee J.H., Kim C., Ko J.H., Ryu S.H., Lee S.G., Yang W.M., Um J.Y., Chinnathambi A., Alharbi S.A., Sethi G, & Ahn K.S. (2017). Ginkgolic Acid C 17:1, Derived from Ginkgo biloba Leaves, Suppresses Constitutive and Inducible STAT3 Activation through Induction of PTEN and SHP-1 Tyrosine Phosphatase. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(2), 276.

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Plasmid Acquisition and Utilization

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Plasmids pLKO.1 puro (#8453, deposited by Bob Weinberg) and pMXs-Stat3-C (#13373, deposited by Shinya Yamanaka) were obtained from Addgene. pLKO.1.shERK5-1 (#0000232396) and pLKO.1.shERK5-2 (#0000010275) were purchased from Sigma-Aldrich. pMX-ERK5 and pMX-DN-ERK5 plasmids were generated by subcloning into pMX vector from pcDNA3-Erk5(WT) and pcDNA3-Erk5AEF vectors, respectively, which were generously provided by Dr Jiing-Dwan Lee (Scripps Research Institute, San Diego, CA).

Fukasawa K., Lyu J., Kubo T., Tanaka Y., Suzuki A., Horie T., Tomizawa A., Osumi R., Iwahashi S., Tokumura K., Murata M., Kobayashi M., Todo T., Hirao A, & Hinoi E. (2023). MEK5-ERK5 Axis Promotes Self-renewal and Tumorigenicity of Glioma Stem Cells. Cancer Research Communications, 3(1), 148-159.

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Capsazepine Inhibits STAT3 Signaling

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Capsazepine (Capz, Figure 1A) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA). A Capz stock solution (100 mM) was prepared using dimethyl sulfoxide, stored at −80°C, and diluted in cell culture medium for use. RPMI 1640, fetal bovine serum (FBS), and an antibiotic-antimycotic mixture were obtained from Thermo Fisher Scientific Inc. (Waltham, MA). Trypan blue was obtained from GIBCO (Grand Island, NY). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Sigma-Aldrich (St. Louis, MO). SDS, Tris, Glycine, and NaCl were obtained from Sigma-Aldrich (St. Louis, MO). Bovine serum albumin was purchased from Biosesang (Sungnam, Korea). Rabbit polyclonal antibody against STAT3, mouse monoclonal antibodies against p-STAT3(Tyr705), p-STAT3(Ser727), p-STAT5(Tyr694), STAT5, Bcl-2, Bcl-xL, PTPε, Caspase-3, Cyclin D1, COX-2, VEGF, MMP-9, Survivin, PARP, and β-actin, goat anti-rabbit IgG-HRP, and goat anti-mouse IgG-HRP were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Antibodies against p-Src(Ty416), Src, p-JAK1(Tyr1022/1023), JAK1, p-JAK2(Tyr1007/1008), and JAK2 were purchased from Cell Signaling Technology (Beverly, MA). pMXS and pMXs-STAT3C were obtained from Addgene (Cambridge, MA). Whole-cell lysates of tumor tissues were prepared using T-PER Tissue Protein Extraction Reagent (Pierce, Rockford, USA).

Lee J.H., Kim C., Baek S.H., Ko J.H., Lee S.G., Yang W.M., Um J.Y., Sethi G, & Ahn K.S. (2016). Capsazepine inhibits JAK/STAT3 signaling, tumor growth, and cell survival in prostate cancer. Oncotarget, 8(11), 17700-17711.

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