Total RNA extracted from seven PC tissue samples and matching normal pancreatic tissues were screened for differentially expressed genes using an Agilent RNA 6000 Nano Kit (Agilent, Santa Clara, CA, USA). In another experiment, total RNA was extracted from three KIF15 knocked-down (KIF15D) samples and three negative control (NC) samples using an Agilent RNA 6000 Nano Kit and a PrimeView Human GeneChip (Agilent) was used for microarray analysis. RNA labelling and hybridisation to Agilent miRNA microarray chips were performed with a GeneChip Hybridization Wash and Stain Kit (Agilent). Microarray data were deposited in the NCBI Gene Expression Omnibus public database (http://www.ncbi.nlm.nih.gov/geo/ ).
Primeview human genechip
Manufactured by Agilent Technologies
Sourced in United States
The PrimeView Human GeneChip is a microarray-based gene expression analysis platform designed for comprehensive transcriptome profiling. It provides a comprehensive coverage of the human genome, enabling researchers to study gene expression patterns across a wide range of biological samples.
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5 protocols using primeview human genechip
1
Differential Gene Expression in Pancreatic Cancer
2
Transcriptomic Analysis of Human Samples
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Total RNA was extracted using the TRIzol method. RNA concentrations were determined with a NanoDrop 2000 spectrophotometer (Thermo Scientific, Pittsburgh, PA, USA). Total RNA from were screened for differentially expressed genes using an Agilent RNA 6000 Nano Kit (Agilent, Santa Clara, CA, USA), and a PrimeView Human GeneChip (Agilent, Santa Clara, CA, USA) was used for microarray analysis. RNA labelling and hybridisation to Agilent miRNA microarray chips were performed with a GeneChip Hybridization Wash and Stain Kit (Agilent, Santa Clara, CA, USA) (17 (link), 18 (link)). The GeneChip data was used to analyze by gene set enrichment analysis (GSEA), and differentially expressed genes were annotated using ingenuity pathway analysis (IPA) to predict path changes.
3
Hypoxia-Induced Gene Expression Profiling
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Total RNA extracted from three normoxia cultured samples and three hypoxia-cultured samples was screened for differentially expressed genes by an Agilent RNA 6000 Nano Kit (Agilent Technologies). Primeview Human GeneChip (Agilent) was used for the analysis. RNA labeling and hybridization to Agilent miRNA microarray chips were performed with a GeneChip Hybridization Wash and Stain Kit (Agilent Technologies). Microarray data were deposited in the NCBI Gene Expression Omnibus public database (http://www.ncbi.nlm.nih.gov/geo/ ).
4
Transcriptome Analysis of SLC6A14 Silencing
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Total RNA was isolated from MKN28-M cells that were transfected with LV-pTZU-SLC6A14 or LV-pTZU6+1 plasmid for 48 h. DEGs in MKN28-M cells with SLC6A14 silence were screened using mRNA microarray analysis via the Prime View Human GeneChip and GeneChip Hybridization Wash and Stain Kit (Agilent). These DEGs were analyzed through NCBI Gene Expression Omnibus Software based on the GSEA database. KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis for these DEGs was implemented using Bioconductor and STRING 9.1 software. The significance levels of gene enrichment in each pathway were analyzed through Fisher's exact test.
5
RNA Extraction and Microarray Analysis
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Using a total RNA extraction kit (Agilent, Santa Clara, CA, USA), we extracted total RNA samples from three HCC and three matched healthy liver tissues (NC). Additionally, total RNA was isolated from three VOPP1 knockdown (VOPP1 KD) and three NC cell samples and underwent microarray analysis using the PrimeView Human GeneChip (Agilent). RNA was then labeled and hybridized using an RNA reverse transcription kit (Agilent).
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