Estrogen pellets (14 d extended release, 0.17 mg β estradiol, Innovative Research of America) were implanted subcutaneouly into 3–4 week old female Balb/C athymic nude mice (Jackson Laboratories). Right and left inguinal mammary fatpads were injected with 106 MCF7 or MDA-MB-361 in 100 μl growth factor reduced Matrigel. Tumors were measured with calipers twice weekly. Tumor-bearing mice were randomized into treatment arms to receive fulvestrant (Novartis) delivered once weekly by intramuscular injection. All animal experimentation was performed in AAALAC approved facilities at Vanderbilt University Medical Center. All animal use protocols were reviewed and approved prior to experimentation by the Institutional Animal Care and Use Committee at Vanderbilt University, Nashville, TN.
Balb c athymic nude mice
Manufactured by Jackson ImmunoResearch
Sourced in United States
BALB/c athymic nude mice are a strain of laboratory mice that lack a functional thymus gland, resulting in a deficiency of T cells. These mice are frequently used as animal models in biomedical research due to their immunodeficient status.
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7 protocols using balb c athymic nude mice
1
Xenograft Tumor Model with Estrogen Implants
2
Subcutaneous Tumor Growth Inhibition
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5 × 106 MCF7 cells were injected subcutaneously in the right hind flank of six female BALB/c athymic nude mice (Jackson Labs, Bar Harbor, ME, USA). When the tumors reached an average of 70 mm3 (d22), PBS (n = 3) or 1 × 108 pfu of KNTc-gD:GDNF∆38 virus (n = 3) was injected intratumorally (single dose/animal). Tumors were measured every several days for 85 d by an animal technician who was blinded to the experimental details, and volumes calculated as (L × W2) × 0.52. If tumor dimension in one orientation exceeded 20 mm, or the tumor volume exceeded 2000 mm3, the mice were humanely euthanized. If mice were unable to ambulate, eat, or drink, lost > 10% body weight, or showed a disheveled appearance they were humanely euthanized. If the cell or virus injections induced redness and inflammation at the injection site, topical antibiotic was administered to minimize risk of topical pathogen infection. All animal studies were approved by the University of Pittsburgh Institutional Animal Care and Use Committee (IACUC protocol 19024419) in accordance with the requirements and recommendations in the NIH Guide for the Care and the Use of Laboratory Animals (Institute for Laboratory Animal Research, 1985).
3
In Vivo Breast Cancer Xenograft Model
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BALB/c athymic nude mice and BALB/c female mice were purchased from the Jackson Laboratory (USA) and maintained in pathogen‐free conditions with a 12 h light/dark cycle. All animal studies were performed according to the guidelines approved by the Animal Ethics Committee of the City University of Hong Kong.
4T1 cells (1 × 105) were injected into the right fourth mammary fat pad of 8‐week‐old female BALB/c mice or BALB/c athymic nude mice. When the tumors reached ≈5 mm in length, the mice were randomly divided into different groups, after which they received either the vehicle control (i.e., PEG400/ethanol/tween80 at 1:1:1) or 6J1 orally every day. The primary tumor size (measured with calipers) and total body weight of each mouse were determined every 5 days. At the endpoint, the mice were euthanized, and the tumors were harvested for further analysis.
4T1 cells (1 × 105) were injected into the right fourth mammary fat pad of 8‐week‐old female BALB/c mice or BALB/c athymic nude mice. When the tumors reached ≈5 mm in length, the mice were randomly divided into different groups, after which they received either the vehicle control (i.e., PEG400/ethanol/tween80 at 1:1:1) or 6J1 orally every day. The primary tumor size (measured with calipers) and total body weight of each mouse were determined every 5 days. At the endpoint, the mice were euthanized, and the tumors were harvested for further analysis.
4
Xenograft Tumor Model with Estrogen Implants
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Estrogen pellets (14 d extended release, 0.17 mg β estradiol, Innovative Research of America) were implanted subcutaneouly into 3–4 week old female Balb/C athymic nude mice (Jackson Laboratories). Right and left inguinal mammary fatpads were injected with 106 MCF7 or MDA-MB-361 in 100 μl growth factor reduced Matrigel. Tumors were measured with calipers twice weekly. Tumor-bearing mice were randomized into treatment arms to receive fulvestrant (Novartis) delivered once weekly by intramuscular injection. All animal experimentation was performed in AAALAC approved facilities at Vanderbilt University Medical Center. All animal use protocols were reviewed and approved prior to experimentation by the Institutional Animal Care and Use Committee at Vanderbilt University, Nashville, TN.
5
Efficacy of Palbociclib on Breast Cancer Xenografts
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Six-week-old female BALB/c athymic nude mice were purchased from Jackson Laboratory. 4×106 MCF-7 PR or MCF-7 PR MITF-depleted cells were subcutaneously injected into the dorsal flank of each mouse. When the tumor volume reached about 100 mm3, all mice were randomized into indicated experiment groups. Palbociclib was administrated intraperitoneally at 25 mg/kg every 2 days for 2 weeks. Saline was used as the control. Relative tumor volumes were calculated with the formula: V = A×(B2)/2, where A and B represent the length and width of each tumor, respectively. For immunohistochemistry (IHC) analysis, tumor samples were sliced and conducted standard IHC staining procedures, and DAB staining was done utilizing SuperPicture™ Polymer Detection Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions. All animal studies were carried out by the National Institutes of Health regulation concerning the care and use of experimental animals and with the approval of the Institutional Animal Care and Use Committees of George Washington University.
6
Nude Mice Xenograft Model for Ovarian Cancer
7
Xenograft Tumor Response to Carboplatin
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Xenograft experiments were performed in 6-week female BALB/c athymic nude mice (Jackson Laboratory) by subcutaneously injecting 5 × 106 IGROV1 cells within 50% Matrigel gelatinous protein mixture (Corning). Mice were randomized to receive treatment after reached a minimum tumor volume of 150 mm3. 3 groups of mice (5 mice per group) were treated intraperitoneally with vehicle (Control), carboplatin (20 mg/kg/2day) and carboplatin (60 mg/kg/2day) for 2 weeks. The investigators were not blinded to the group allocation and data collection. All animal experiments were conducted in accordance with the Institution Animal Care and Use Committee of the George Washington University for laboratory animal use and care, and all relevant ethical regulations were followed.
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