Wild type balb c mice

The starvation experiment was as follows: 8- to 10-week-old male C57BL/6Crl, 129S2/SvPas, BALB/c wild-type mice, and Creb3l3-/- null mice (The Jackson Laboratory, Bar Harbor, ME) were allowed free access to water and fed a standard, iron-balanced chow diet in pellets (2018S Teklad Global 18% Protein Rodent Diet; Harlan Laboratories, (San Pietro Al Natisone, UD, Italy); iron content, 225 mg/kg) or starved up to 48 hours starting at the beginning of the light cycle.
Iron-deficient diet experiments were as follows: 8-week-old male C57BL/6Crl wild-type mice were fed an iron-deficient diet (ssniff EF R/M Iron Deficient; Charles River, Calco, LC, Italy; iron content, <10 mg/kg) for 9 days before death, or for 6 days before the 24- to 48-hour starvation period.
All animals received humane care according to the criteria outlined by the Federation of European Laboratory Animal Science Associations. The study was approved by the Ethics Committee for Animal Studies at the University of Modena and Reggio Emilia.

Rag2‐deficient mice57 were bred and maintained in the Sunnybrook Research Institute Comparative Research facility in specific pathogen‐free conditions. C57BL/6 and BALB/c wild‐type mice were obtained from Jackson Laboratories. All animal procedures were approved by the Sunnybrook Research Institute Animal Care Committee (Toronto, Ontario, Canada).

CXCR2 knockout (BALB/C‐Cxcr2^tm1Mwm) mice, BALB/C wild‐type mice, and C57BL/6‐Tg[CAG‐EGFP]1Osb/J mice were obtained from the Jackson Laboratory (Bar Harbor, ME). C57BL/6J wild‐type male mice (8‐ to 10‐week old; 22–25 g) were purchased from Orient, Co., Ltd. (Gapyeong, Republic of Korea). All animals were housed in an air‐conditioned facility (22°C–25°C; relative humidity 50%–65%) and provided a laboratory diet and water. Animal treatment and maintenance were performed in accordance with the Principles of Laboratory Animal Care, and animal experiments were performed using protocols approved by the Pusan National University Institutional Animal Use and Care Committee (PNU‐2016‐1381).

Animal procedures and experiments were approved by the University of Massachusetts Amherst IACUC in accordance with the U.S. Public Health Service Policy and NIH Guide for the Care and Use of Laboratory Animals. Brains were collected from 4–6 week old male and female BALBc/nude, or BALBc wild type mice (Jackson Laboratories). Mice were anesthetized using isoflurane, euthanized by decapitation, and brains were removed and immediately used for NIC and organotypic brain slice preparation.