Cy3 conjugated anti mouse igg
Cy3-conjugated anti-mouse IgG is a secondary antibody that binds to mouse immunoglobulin G (IgG) and is labeled with the fluorescent dye Cy3. This antibody can be used in various immunodetection techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to visualize and detect the presence of mouse IgG in biological samples.
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8 protocols using cy3 conjugated anti mouse igg
Immunofluorescence Staining of Cellular Proteins
Immunostaining of HTNV-infected A549 cells
Immunofluorescence Staining of Cellular Proteins
Immunocytochemical Analysis of ECFCs
Visualizing VSV-G and Ezrin Localization
Immunofluorescence Staining of Flagellar Proteins
Immunofluorescence Detection of Protein Interactions
Cell cultures overexpressing only fluorescent fusion proteins were directly mounted with SlowFade reagent (Molecular Probes, Thermo Fisher Scientific, USA). Cell cultures overexpressing HA-DLG1 were permeabilized with TritonX-100 0.1% in PBS after fixation, and incubated overnight with anti-HA (12CA5 clone, Sigma Aldrich, St. Louis, MO, USA). Cy3 conjugated anti-mouse IgG, was used as secondary antibody (Chemicon International, Temecula, CA, USA).
In all fluorescent microscopy experiments, despite the different spectral properties of the tags eventually used for protein detection, E6 expression was shown in green, E7 expression in blue and DLG1 expression in red. The Pearson correlation coefficient (PCC), used as co-localization parameter, was performed employing the Coloc2 plugin from FIJI software [27 (link)].
Fluorescence microscopy images were collected with a Carl Zeiss LSM880 confocal microscope following the sequential acquisition mode (Carl Zeiss, Germany). A 63x NA 1.4 plan apochromat oil immersion objective was employed.
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Immunofluorescence analysis of DLG1, GM130, and γ-Tubulin
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