Human cxcl10 ip 10 quantikine elisa kit

Manufactured by R&D Systems

Sourced in United States

The Human CXCL10/IP-10 Quantikine ELISA Kit is a quantitative sandwich enzyme immunoassay designed to measure human CXCL10/IP-10 levels in cell culture supernatants, serum, and plasma.

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Lab products found in correlation

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20 protocols using human cxcl10 ip 10 quantikine elisa kit

Quantification of IL-1β and CXCL10 using ELISA

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Recombinant Human IL-1 beta and Recombinant Human CXCL10/IP-10 from R& D Systems (R& D Systems™) were reconstituted according to the manufacturer’s instructions. Following the reconstitution, the analytes were diluted to generate their respective stock solution.
The stock solutions were used to generate the following standard curves:
Concentrations (pg/mL): 1000, 500, 250,125, 62.5, 31.25.
Human IL-1 beta/IL-1 F2 Quantikine ELISA Kit and Human CXCL10/IP-10 Quantikine ELISA Kit (R& D Systems™) were used to quantify the analytes for further comparison with the Proteome Profiler™ outcome.

Altara R., Manca M., Hessel M.H., Janssen B.J., Struijker-Boudier H.H., Hermans R.J, & Blankesteijn W.M. (2014). Improving membrane based multiplex immunoassays for semi-quantitative detection of multiple cytokines in a single sample. BMC Biotechnology, 14, 63.

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MAFB Knockdown Impact on Macrophage Responses

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MAFB-KD or control macrophages were washed with ice-cold PBS and lysed with lysis buffer containing 50 mM HEPES (pH 7.2), 150 mM NaCl, 1% Triton X-100 and Roche Complete EDTA Free Protease Inhibitor Cocktail. Cell lysates were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and then subjected to immunoblot analysis using an anti-MAFB (1:1000 v/v; Proteintech, #20189-1-AP) or anti-GAPDH (1:6000 v/v; Medical and Biological Laboratories, #M171-3) antibody. Band intensities were quantified using Image Lab Software version 6.0 (Bio-Rad).
The concentrations of secreted MCP-1 and IP-10 from MAFB-KD and control macrophages infected with Mtb were measured by a Human CCL2/MCP-1 Quantikine ELISA Kit and a Human CXCL10/IP-10 Quantikine ELISA Kit (R&D Systems), respectively. The culture media from Mtb-infected macrophages were collected at 4 and 24 h postinfection (p.i.), followed by filtration with a 0.45-μm pore size filter (Toyo Roshi Kaisha).

Hikichi H., Seto S., Wakabayashi K., Hijikata M, & Keicho N. (2022). Transcription factor MAFB controls type I and II interferon response-mediated host immunity in Mycobacterium tuberculosis-infected macrophages. Frontiers in Microbiology, 13, 962306.

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Cytokine Profiling of Umbilical Cord Blood

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The umbilical cord blood plasma was assessed for concentrations of IL-6, IL-1β, TNF, and IL-8 using sensitive and specific V-PLEX immunoassays (Meso Scale Discovery, Gaithersburg, MD, USA), according to the manufacturer’s instructions. CXCL10 was assessed using the Human CXCL10/IP-10 Quantikine ELISA Kit (R&D Systems, Minneapolis, MN, USA), according to the manufacturer’s instructions.

Para R., Romero R., Miller D., Galaz J., Done B., Peyvandipour A., Gershater M., Tao L., Motomura K., Ruden D.M., Isherwood J., Jung E., Kanninen T., Pique-Regi R., Tarca A.L, & Gomez-Lopez N. (2021). The Distinct Immune Nature of the Fetal Inflammatory Response Syndrome Type I and Type II. ImmunoHorizons, 5(9), 735-751.

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Cytokine Quantification in Conditioned Media

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The expression levels of visfatin, CXCL1, PAI-1 and CXCL10 in the CM were detected by using Human PBEF/Visfatin DuoSet ELISA (DY4335-05, R&D Systems), Human CXCL1/GRO alpha Quantikine ELISA Kit (DGR00B, R&D Systems), Human Serpin E1/PAI-1 Quantikine ELISA Kit (DSE100, R&D Systems), and Human CXCL10/IP-10 Quantikine ELISA Kit (DIP100, R&D Systems), according to the manufacturers’ instructions.

Wang Y.Y., Chen H.D., Lo S., Chen Y.K., Huang Y.C., Hu S.C., Hsieh Y.C., Hung A.C., Hou M.F, & Yuan S.S. (2020). Visfatin Enhances Breast Cancer Progression through CXCL1 Induction in Tumor-Associated Macrophages. Cancers, 12(12), 3526.

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Validating IP-10 LFA Against HIV RNA

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Plasma HIV RNA levels (VL result) were determined according to routine practice using the Abbott Real‐Time HIV1/2 Polymerase Chain Reaction (reference test). Quantitative measurement of IP‐10 concentrations in plasma samples was conducted at Mondial Diagnostics using the Human CXCL10/IP‐10 Quantikine ELISA kit (R&D Systems), according to the manufacturer's specifications. Plasma specimens were also blindly assayed with the IP‐10 LFA using 1 μl of plasma sample from the same test batch as the one used for capillary blood at study sites. The outcome was determined by Cube Reader as well as visually by two independent readers (scale: 0–4, 0.5 increments). When the Cube Reader results differed ≥2‐fold between plasma and capillary blood, the IP‐10 LFA was repeated, and the average result of both tests was included in the analysis. The IP‐10 LFA was validated using both capillary blood and plasma against HIV RNA levels and ELISA IP‐10 measurements.

Saura‐Lázaro A., Bock P., van den Bogaart E., van Vliet J., Granés L., Nel K., Naidoo V., Scheepers M., Saunders Y., Leal N., Ramponi F., Paulussen R., de Wit T.R., Naniche D, & López‐Varela E. (2023). Field performance and cost‐effectiveness of a point‐of‐care triage test for HIV virological failure in Southern Africa. Journal of the International AIDS Society, 26(10), e26176.

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Quantifying Plasma CXCL10 Levels

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CXCL10 levels in plasma were quantified using the human CXCL10/IP-10 Quantikine ELISA Kit (R&D Systems Inc, Minneapolis, MN) according to the manufacturer’s protocol.

Rosenberg B.R., Depla M., Freije C.A., Gaucher D., Mazouz S., Boisvert M., Bédard N., Bruneau J., Rice C.M, & Shoukry N.H. (2018). Longitudinal transcriptomic characterization of the immune response to acute hepatitis C virus infection in patients with spontaneous viral clearance. PLoS Pathogens, 14(9), e1007290.

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Quantifying Inflammatory and Coagulation Markers

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Serum and soluble inflammatory and coagulation markers were obtained using commercially available kits. Assays were performed according to the manufacture’s protocols. The following markers were measured in blood: pro-inflammatory cytokines serum IL-6 (Human IL-6 Qunatikine HS Elisa Kit), serum IL-18 (Human IL-18/IL-1 F4 ELISA), soluble TNF-α receptor-1 (TNFR1) (Human sTNF RI/TNFRSF1A Quantikine ELISA Kit), and soluble TNF-α receptor-2 (TNFR2) (Human sTNF RII/TNFRSF1B Quantikine ELISA Kit), Interferon gamma-induced protein-10 (IP-10)(Human CXCL10/IP-10 Quantikine ELISA Kit), soluble CD14 (Human CD14 Quantikine ELISA Kit) (all R&D System, Minneapolis, MN, USA) and pro-inflammatory protein C-reactive protein (CRP) (EIA kit, Cayman Chemical Company, Ann Arbor, MI, USA), Serum Amyloid A (SAA) (ELISA, Assaypro, St. Charles, MO, USA) and were also measured. Inflammatory index is a calculated parameter: 0.333*log (IL-6) + 0.666*log (sTNFR1). The following markers of thrombosis and coagulation were also measured: Plasminogen activator inhibitor-1 (PAI-1) (AssayMax Human PAI-1 ELISA kit, Assaypro, St. Charles, MO, USA), D-dimer (ELISA ZYMUTEST DDimer, ANIARA, West Chester, OH, USA) and Fibrinogen (immunoperoxidase assay, GenWay, San Diego, CA, USA).

Van Epps P., Oswald D., Higgins P.A., Hornick T.R., Aung H., Banks R.E., Wilson B.M., Burant C., Graventstein S, & Canaday D.H. (2016). Frailty has a stronger association with inflammation than age in older veterans. Immunity & Ageing : I & A, 13, 27.

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Quantification of Serum IP-10 Levels

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Quantitative determination of human IP-10 (overall IP-10, no differentiation of aIP-10 or cIP-10) in serum was carried out using the HK311 human IP-10 ELISA KIT (Hycult biotech, PA). Measurable concentration range is from 20 to 5,000 pg/mL. Serum samples were diluted (1:4) as described in the protocol and the absorbance was measured at 450 nm.
IP-10 levels in serum samples of the FU cohort (27 patients with chronic HCV infection and 17 HC) were measured as overall IP-10, aIP-10 and c-IP10 by diagnostic lab, Myriad-Rules Based Medicine (Austin, USA). In 18 patients overall IP-10 was under the lower limit of quantification of this assay. These patients were included in the analysis with the value of the lower limit of quantification (212 pg/mL). In the cholestatic non-HCV validation cohort (n = 16) IP-10 serum concentration was measured by using the Human CXCL10/IP-10 Quantikine ELISA kit (R&D Systems, Minneapolis, USA) measuring overall IP-10 (no differentiation of aIP-10 or cIP-10). The ELISA was performed according to the manufacturer’s instructions.

Rau M., Schmitt J., Berg T., Kremer A.E., Stieger B., Spanaus K., Bengsch B., Romero M.R., Marin J.J., Keitel V., Klinker H., Tony H.P., Müllhaupt B, & Geier A. (2018). Serum IP-10 levels and increased DPPIV activity are linked to circulating CXCR3+ T cells in cholestatic HCV patients. PLoS ONE, 13(12), e0208225.

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Quantifying Human CXCL10 in Mouse Serum

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Human CXCL10 was measured in 1:5 diluted mouse serum samples using the Human CXCL10/IP10 Quantikine ELISA Kit (R&D Systems, Minneapolis, MN, USA) according to manufacturer’s protocol.

van de Garde M.D., Pas S.D., van Oord G.W., Gama L., Choi Y., de Man R.A., Boonstra A, & Vanwolleghem T. (2017). Interferon-alpha treatment rapidly clears Hepatitis E virus infection in humanized mice. Scientific Reports, 7, 8267.

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Poly(I:C) dsRNA Activation Assay

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For Poly(I:C) dsRNA treatment experiments, H69ARsgCTRL and H69ARsgMAVS cells were plated in RPMI media, transfected with 0.5 μg/mL Poly(I:C) HMW (InvivoGen, Sand Diego, CA) using XtremeGene HP transfection reagent (Sigma-Aldrich, St. Louis, MO) and cultured for 72 hours. On day 3 after transfection, conditioned media was recovered and CXCL10 protein expression was quantified using Human CXCL10/IP-10 Quantikine ELISA kit (R&D Systems, Minneapolis, MN). RNA was extracted and expression levels of relevant genes were analyzed by qRT-PCR.

Cañadas I., Thummalapalli R., Kim J.W., Kitajima S., Jenkins R.W., Christensen C.L., Campisi M., Kuang Y., Zhang Y., Gjini E., Zhang G., Tian T., Sen D.R., Miao D., Imamura Y., Thai T., Piel B., Terai H., Aref A.R., Hagan T., Koyama S., Watanabe M., Baba H., Adeni A.E., Lydon C.A., Tamayo P., Wei Z., Herlyn M., Barbie T.U., Uppaluri R., Sholl L.M., Sicinska E., Sands J., Rodig S., Wong K.K., Paweletz C.P., Watanabe H, & Barbie D.A. (2018). Tumor innate immunity primed by specific interferon-stimulated endogenous retroviruses. Nature medicine, 24(8), 1143-1150.

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