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Nephrat

Manufactured by Exocell
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Sourced in United States

The Nephrat is a compact and versatile laboratory equipment designed for precise fluid handling. It features an integrated peristaltic pump capable of accurately dispensing and aspirating a wide range of fluid volumes. The Nephrat is a reliable tool for various applications in research and clinical settings.

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Lab products found in correlation

Cyclic amp select elisa kit, by Cayman Chemical (1 mentions) Creatinine companion, by Exocell (1 mentions) Labmax, by Labtest (1 mentions) Albuwell, by Exocell (1 mentions) Creatinine assay kit, by Cayman Chemical (1 mentions) Aldosterone elisa kit, by Abcam (1 mentions)

4 protocols using nephrat

1

Electrolyte and Fluid Biomarkers

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Urine and plasma electrolytes were analysed using the Cobas Integra 400 Chemistry Analyzer (Block Scientific, NY). Osmolality was determined using a µOSMETTE freeze point osmometer (Precision Systems, MA). Urinary albumin, creatinine, and cyclic AMP (cAMP) were evaluated using commercially available kits (Nephrat (Exocell, PA); Creatinine Companion (Exocell, PA); Cyclic AMP Select ELISA Kit (Cayman chemical, MI) respectively. Plasma AVP levels were determined using Vasopressin Direct RIA (Buhlmann Laboratories, Switzerland). Urinary electrolytes and cAMP were normalized for flow normalized to body weight.
Dorey E.S., Walton S.L., Kalisch‐Smith J.I., Paravicini T.M., Gardebjer E.M., Weir K.A., Singh R.R., Bielefeldt‐Ohmann H., Anderson S.T., Wlodek M.E, & Moritz K.M. (2019). Periconceptional ethanol exposure induces a sex specific diuresis and increase in AQP2 and AVPR2 in the kidneys of aged rat offspring. Physiological Reports, 7(21), e14273.
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2

Quantifying Urine Protein and Albumin

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The total urine protein levels were quantified using an automated analyzer with a commercial assay (Labmax®, Labtest, Lagoa Santa, Brazil). The albumin levels were determined by ELISA (Nephrat, Exocell, Philadelphia, PA, USA) following the manufacturer’s instructions.
de Oliveira A.A., de Oliveira T.F., Bobadilla L.L., Garcia C.C., Berra C.M., de Souza-Pinto N.C., Medeiros M.H., Di Mascio P., Zatz R, & de M. Loureiro A.P. (2017). Sustained kidney biochemical derangement in treated experimental diabetes: a clue to metabolic memory. Scientific Reports, 7, 40544.
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3

Measuring Albuminuria in Animal Models

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Albuminuria was measured by Nephrat or Albuwell (Exocell Inc., Philadelphia, PA, USA) using 24‐h urine collection samples from animals housed in individual metabolic cages.
Mima A., Yasuzawa T., King G.L, & Ueshima S. (2018). Obesity‐associated glomerular inflammation increases albuminuria without renal histological changes. FEBS Open Bio, 8(4), 664-670.
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4

Biomarkers of Kidney Dysfunction and Oxidative Stress

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Blood samples were centrifuged for 5 min at 3000 rpm and were stored at − 80 °C until analysis. Serum creatinine, blood urea nitrogen, albumin, total cholesterol, calcium, phosphorus, and glucose levels were measured using a Fuji Dri-Chem 3500 (Fujifilm Japan, Tokyo, Japan). Urinary albumin, creatinine, and neutrophil gelatinase-associated lipocalin (NGAL) levels were measured using an enzyme-linked immunosorbent assay (ELISA) kit (albumin: Nephrat, Exocell, Philadelphia, PA, USA; creatinine: Creatinine Assay Kit, Cayman Chemical, Ann Arbor, MI, USA; NGAL: Lipocain-2 Rat ELISA Kit, Abcam, Cambridge, MA, USA). The urinary 8-hydroxydeoxyguanosine (8-OHdG), a sensitive indicator of oxidative DNA damage, was determined using a sandwich ELISA kit (Japan Institute for Control of Aging, Shizuoka, Japan). Serum aldosterone levels were measured using an Aldosterone ELISA kit (Abcam, Cambridge, UK).
Goto S., Fujii H., Watanabe K., Shimizu M., Okamoto H., Sakamoto K., Kono K, & Nishi S. (2023). Renal protective effects of astragalus root in rat models of chronic kidney disease. Clinical and Experimental Nephrology, 27(7), 593-602.
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