Trifluoroethanol tfe

Manufactured by Merck Group

Sourced in United States

Trifluoroethanol (TFE) is a colorless, volatile organic compound with the chemical formula CF3CH2OH. It is a polar, aprotic solvent used in various laboratory applications, including as a reagent and an additive in chemical reactions. TFE exhibits high solvating power and is often used in the synthesis of organic compounds and in the study of protein structures.

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Lab products found in correlation

Sta449f3, by Netzsch (1 mentions) Eclipse ti, by Nikon (1 mentions) Microplate reader, by Thermo Fisher Scientific (1 mentions) Jem 2100f, by JEOL (1 mentions) Leo 1430vp, by Zeiss (1 mentions) Ethylenediaminetetraacetic acid edta, by Thermo Fisher Scientific (1 mentions) 1 2 dioleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) 1 2 dioleoyl sn glycero 3 phosphoethanolamine, by Avanti Polar Lipids (1 mentions) N 2 hydroxyethylpiperazine n 2 ethanesulfonic acid hepes, by Merck Group (1 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) Hexafluoroisopropanol hfip, by Merck Group (1 mentions) Celltiter glo reagent, by Promega (1 mentions) Acetic acid, by Merck Group (1 mentions) Tetrakis triphenylphosphine palladium, by Merck Group (1 mentions) Chlorotrityl chloride resin ctc, by Chem-Impex (1 mentions) Triisopropyl silane tips, by Protein Technologies (1 mentions) N methylmorpholine nmm, by Merck Group (1 mentions) Facsaria, by BD (1 mentions) Polymyxin b, by Merck Group (1 mentions) Dibac4 3, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

2,2,2-trifluoroethanol (TFE) (29 citations) Trifluoroethanol (20 citations) Ammonium bicarbonate (ABC),2,2,2,-trifluoroethanol(TFE), (2 citations) Solvent 2,2,2-trifluoroethanol (TFE) (2 citations)

11 protocols using trifluoroethanol tfe

Electrospun PLLA/HA Nanofibrous Scaffold

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Polylactic acid (PLLA) was purchased from Jinan Daigang Biomaterial Co., Ltd., China, Mw(PLLA) = 100 kDa. Hydroxyapatite (HA) nanowire was from Suruiyunduan New Material Co., Ltd., China, the average length was 209 nm and the average diameter was 64 nm (Figs. S1 and S3). Trifluoroethanol (TFE) was from Sigma-Aldrich, US. Gelatin (Gel) was from Beijing Chemical Plant, China, Mw (gel) = 50–100 kDa. TL01 Electrospinning Machine was from Shenzhen Tongli Micro-Nano Technology Co., Ltd., China. Scanning electron microscope (SEM, LEO-1430VP) was from Carl Zeiss, Germany. Transmission electron microscope (TEM, JEM-2100F) was from JEOL, Japan. Universal mechanical test instrument (UTM6104) was from Shenzhen Suns Technology Co., Ltd., China. Water contact angle analysis instrument (SDC-200) was from Dongguan Sindin Precision Instrument Co., Ltd., China. Thermogravimetric analysis instrument (STA 449F3) was from NETZSCH, Germany. Confocal microscope (ECLIPSE Ti) was from Nikon, Japan and microplate reader was from Thermo Fisher Scientific, US. Atomic absorption spectrophotometer (AAS, Z-2000, Japan).

Liu F., Wang X., Chen T., Zhang N., Wei Q., Tian J., Wang Y., Ma C, & Lu Y. (2019). Hydroxyapatite/silver electrospun fibers for anti-infection and osteoinduction. Journal of Advanced Research, 21, 91-102.

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Purification and Solubilization of NSP6 Peptide

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The NSP6 peptide (residues 91–112) “-NH2-VMRIMTWLDMVDTSLSGFKLKD–COOH–”, with purity >88% was purchased from Gene script, USA. Organic solvents such as Trifluoroethanol (TFE) with ≥99% purity was purchased from Sigma-Aldrich. Lyophilized peptide was dissolved in 10 mM sodium phosphate buffer (pH 7.0) at a concentration of 10 μM.

Kumar A., Kumar P., Saumya K.U, & Giri R. (2021). Investigating the conformational dynamics of SARS-CoV-2 NSP6 protein with emphasis on non-transmembrane 91–112 & 231–290 regions. Microbial Pathogenesis, 161, 105236.

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Phospholipid and Labeled Amino Acid Synthesis

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1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine
(POPC), 1,2-dioleoyl-sn-glycero-3-phosphocholine
(DOPC), and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine
(DOPE) were purchased from Avanti Polar Lipids (Alabaster, AL). The
phospholipids were dissolved in chloroform and stored at −20
°C before use. Trifluoroethanol (TFE) and N-[2-hydroxyethyl]piperazine-N′-2-ethane sulfonic acid (HEPES) were purchased
from Sigma-Aldrich (St. Louis, MO). Ethylenediaminetetraacetic acid
(EDTA) and sodium chloride (NaCl) were purchased from Fisher Scientific
(Pittsburgh, PA). Prephosphorylated Fmoc-serine and Fmoc amino acids
were obtained from Applied Biosystems (Carlsbad, CA). ¹³C=O labeled Fmoc-alanine, ¹³C=O labeled
Fmoc-leucine, ¹⁵N labeled Fmoc-alanine, and ¹⁵N labeled Fmoc-leucine were purchased from Isotec TM/Sigma-Aldrich
(Miamisburg, OH). All other peptide synthesis and HPLC reagents and
solvents were acquired from VWR (San Dimas, CA). NMR glass sample
cells were purchased from New Era Enterprise (Vineland, NJ). Glass
plates were purchased from Superior Marienfeld Laboratory Glassware
(Lauda-Konigshofen, Germany).

Yu X, & Lorigan G.A. (2014). Secondary Structure, Backbone Dynamics, and Structural Topology of Phospholamban and Its Phosphorylated and Arg9Cys-Mutated Forms in Phospholipid Bilayers Utilizing 13C and 15N Solid-State NMR Spectroscopy. The Journal of Physical Chemistry. B, 118(8), 2124-2133.

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Aβ42 Peptide and Quantification

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Human synthetic peptide Aβ₄₂ and internal standard (uniformly labeled, Nitrogen-15) Aβ₄₂ both with trifluoracetate as counter ion, were purchased from r-Peptide. Certificate of Analysis from rPeptide contained information about Aβ₄₂ purity (>97% for Aβ₄₂ and > 95% for ¹⁵N-Aβ₄₂), amino acid sequence and molecular weight assessment by Maldi TOF mass spectrometry.
Accuracy of the weight of this material is based on amino acid analysis of the standards used for HPLC analyses of each lot of material (Jane Wightman, personal communication). Discarded human CSF samples were obtained from the clinical laboratory at the Hospital of the University of Pennsylvania (Philadelphia, PA, USA). Purchases of aCSF perfusion fluid (Na 150 mM, K 3.0 mM, Ca 1.4 mM, Mg 0.8 mM, P 1.0 mM, and Cl 155 mM) and normal rat plasma were from Harvard Apparatus and GeneTex, respectively. Guanidine hydrochloride (GuCl) and trifluoroethanol (TFE) were purchased from Sigma-Aldrich. All other chemical reagents were purchased from Fisher Scientific.

Korecka M., Waligorska T., Figurski M., Toledo J.B., Arnold S.E., Grossman M., Trojanowski J.Q, & Shaw L.M. (2014). Qualification of a Surrogate Matrix-Based Absolute Quantification Method for Amyloid-β42 in Human Cerebrospinal Fluid Using 2D UPLC-Tandem Mass Spectrometry. Journal of Alzheimer's disease : JAD, 41(2), 441-451.

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Amyloid Peptide Preparation and Characterization

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The peptides Aβ40, Aβ42, and Aβ43 were purchased from Peptides International (Peptide Institute, Inc., Osaka, Japan). The peptides were TFA salts. Hexafluoroisopropanol (HFIP) and trifluoroethanol (TFE) were purchased from Sigma (St. Louis, MO) and Sigma-Aldrich (St. Louis, MO), respectively. Identities of the peptides were confirmed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Pachahara S.K., Adicherla H, & Nagaraj R. (2015). Self-Assembly of Aβ40, Aβ42 and Aβ43 Peptides in Aqueous Mixtures of Fluorinated Alcohols. PLoS ONE, 10(8), e0136567.

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Preparation of NSP1 Peptide Stock

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The NSP1 peptide (residues 131–180), corresponding to the C-terminal regions with purity>82% was purchased from Gene script, USA. Organic solvents such as Trifluoroethanol (TFE) with ≥99% purity were purchased from Sigma-Aldrich. Lyophilized peptide was dissolved in ultra-pure water at a concentration of 1 mg/ml and calculated for its micromolar concentration, i.e., 184 μM, used as a stock for experimental working concentration.

Kumar A., Kumar A., Kumar P., Garg N, & Giri R. (2021). SARS-CoV-2 NSP1 C-terminal (residues 131–180) is an intrinsically disordered region in isolation. Current Research in Virological Science, 2, 100007.

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Peptide Synthesis Protocol for Drug Discovery

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All chemicals, reagents, resins, and cell lines were obtained from commercial sources. Fmoc-protected amino acids, 2-(6-chloro-1H-benzotriazole-1-yl)-1,1,3,3-tetramethylaminium hexafluorophosphate (HCTU) and trifluoroacetic acid (TFA) were purchased from Advanced ChemTech, Louisville, KY. Chlorotrityl chloride resin (CTC) was purchased from ChemImpex, Wood Dale, IL. Diisopropylethylamine (DIEA), methanol (MeOH), chloroform, acetic acid, trifluoroethanol (TFE), tetrakis (triphenylphosphine) palladium, N-methyl morpholine (NMM), and 6-carboxyfluorescein (6-FAM) were purchased from Sigma-Aldrich, St. Louis, MO. Dimethylformamide (DMF), dichloromethane (DCM) and triisopropyl silane (TIPS) were purchased from Protein Technologies, Tucson, AZ. (7-Azabenzotriazol-1-yloxy) tripyrrolidinophosphonium hexafluorophosphate (PyAOP), (benzotriazol-1-yloxy) tripyrrolidinophosphonium hexafluorophosphate (PyBOP), and N-hydroxybenzotriazole (HOBT) were purchased from EMD Millipore, Billerica, MA. Cell lines and their respective media were purchased from the American Type Culture Collection (ATCC, Manassas, VA). CellTiter-Glo^® reagent was purchased from Promega (Madison, WI).

Pallerla S., Naik H., Singh S., Gauthier T., Sable R, & Jois S.D. (2018). Design of cyclic and D-amino acids containing peptidomimetics for inhibition of protein-protein interactions of HER2-HER3. Journal of peptide science : an official publication of the European Peptide Society, 24(2), 10.1002/psc.3066.

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Peptide Synthesis and Analysis Protocol

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Fmoc-amino acids, CLEAR amide resin, 2-(1H-benzotriazol-1-yl)-1,1,3,3,-tetramethyl uronium hexaflurophosphate (HBTU),4-[2,4-dimethoxyphenyl) (Fmoc-amino) methyl] phenoxyacetic acid and hydroxybenzotriazole (HOBt) were obtained from Peptide International, USA. Tri-isopropyl silane (TIS) and trifluro acetic acid (TFA), thioanisole, histopaque, ethanedithiol (EDT), NPN, SYTOX green, Polymyxin B, trifluoroethanol, (TFE) and diisopropylethylamine (DIEA) were purchased from Sigma-Aldrich Corporation, USA. [DiBAC₄ (3)] was obtained from Molecular probes, Eugene. Piperidine and solvents (HPLC grade) were purchased from Merck, India. HPLC purification and analysis was done on a Shimadzu instrument using C18 column. Mass spectra were recorded on a MALDI-TOF mass spectrometer (AB Sciex 4800 plus). Flow cytometry analysis was carried out on a FACS Aria instrument, BD, Sparks. All other reagents used are of analytical grade procured from the local suppliers.

Abraham P., Sundaram A., R A., V R., George S, & Kumar K.S. (2015). Structure-Activity Relationship and Mode of Action of a Frog Secreted Antibacterial Peptide B1CTcu5 Using Synthetically and Modularly Modified or Deleted (SMMD) Peptides. PLoS ONE, 10(5), e0124210.

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Synthesis of Polymer Brushes on Ti6Al4V

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Methacryloyl chloride (97 %), 4-methylphenol (99 %), propargyl alcohol (99 %), phenylacetylene (98 %), ethyl α-bromoisobutyrate (EBiB, 98 %), α-bromoisobutyryl bromide (BiBB, 98 %), 2, 2′-bipyridyl (BPY, 97 %), copper(I) bromide (CuBr, 99.999 %), sodium azide (99.5 %), trifluoroethanol (TFE, 99 %), benzene (99.8 %) and dimethylformamide (DMF, 99.5 %) were purchased from Sigma-Aldrich (St. Louis, MO, USA) and used as received. Diethyl (hydroxymethyl)phosphonate (P-OH, 98 %) and 2-(2-(2-Chloroethyoxy)ethoxy)-ethanol (TEG-Cl, 96 %) were purchased from TCI America (Portland, OR, USA) and used as received. Triethylamine (TEA, 99.5 %, Sigma-Aldrich) was dried by calcium hydride (CaH₂, 99.99 %, Sigma-Aldrich) and distilled prior to use. Ti6Al4V plates (1.3 mm thick, Titanium Metal Supply Inc., Poway, CA, USA) was cut into 10 × 10 mm² square pieces, which were sequentially polished under water with 600, 1500, and 3000 grit silicon carbide sandpapers and ultrasonically cleaned with hexane (10 min), DCM (10 min), and acetone (10 min) prior to use.

Liu P, & Song J. (2015). Well-controlled ATRP of 2-(2-(2-Azidoethyoxy)ethoxy)ethyl Methacrylate for High-density Click Functionalization of Polymers and Metallic Substrates. Journal of polymer science. Part A, Polymer chemistry, 54(9), 1268-1277.

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Comprehensive Chemical Reagents for Mass Spectrometry

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LC/MS-grade water, methanol, and acetonitrile (ACN) were used in this study if not otherwise stated and were purchased together with chloroform, 25% (v/v) NH₄OH, CaCl₂, KCl, KH₂PO₄, MgCl₂, NaCl, NaHCO₃, NaHPO₄, sucrose, and glucose from Merck, Darmstadt, Germany. Trifluoroethanol (TFE), triethylammonium bicarbonate buffer (TEAB), formic acid (FA), EGTA, PM400-Ficoll (Ficoll), NADP, L-Glutamic dehydrogenase from bovine liver (GluDH), guanidine hydrochloride, tris(2-carboxyethyl)phosphine (TCEP), chloroacetamide (CAA), Triton-X100, and MS-grade trypsin were obtained from Sigma-Aldrich, Taufkirchen, Germany. HEPES was purchased from VWR Chemicals, Darmstadt, Germany. SDS was purchased from Serva Electrophoresis GmbH, Heidelberg, Germany. TFA was obtained from Roth, Karlsruhe, Germany. Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase (PNGase F) was obtained from Roche, Mannheim, Germany. Rapigest was obtained from Waters, Milford, USA. Pierce 660 nm protein assay, Halt Protease and phosphatase inhibitor cocktail, and TMTsixplex isobaric labeling reagents (TMT6) were purchased from Thermo Fisher Scientific, Bleiswijk, Netherlands. C. botulinum strain A–D cell culture supernatants (BoNT A, B, C1, D) were obtained from Miprolab, Göttingen, Germany.

Silbern I., Pan K.T., Fiosins M., Bonn S., Rizzoli S.O., Fornasiero E.F., Urlaub H, & Jahn R. (2021). Protein Phosphorylation in Depolarized Synaptosomes: Dissecting Primary Effects of Calcium from Synaptic Vesicle Cycling. Molecular & Cellular Proteomics : MCP, 20, 100061.

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