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Fitc labeled goat anti mouse igg

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FITC-labeled goat anti-mouse IgG is a secondary antibody that binds to mouse immunoglobulin G (IgG) and is labeled with the fluorescent dye fluorescein isothiocyanate (FITC). This antibody can be used in various immunoassays and imaging techniques to detect and visualize the presence of mouse IgG in samples.

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Lab products found in correlation

Cy3 labeled goat anti rabbit igg, by Beyotime (4 mentions) 4 6 diamidino 2 phenylindole (dapi), by Beyotime (4 mentions) Manganese chloride tetrahydrate, by Merck Group (1 mentions) Bovine serum protein bsa, by Merck Group (1 mentions) Dulbecco s modified eagle s medium dmem, by Keygen Biotech (1 mentions) Fetal bovine serum (fbs), by Keygen Biotech (1 mentions) Sp2 confocal microscope, by Leica (1 mentions) Acc 025, by Alomone (1 mentions) Cm1950 cryostat, by Leica (1 mentions) Quickblock blocking buffer, by Beyotime (1 mentions) Eclipse ti microscope, by Nikon (1 mentions) Digital image capture system, by Olympus (1 mentions) Cy3 labeled goat anti rabbit igg antibody, by Beyotime (1 mentions) Inverted fluorescence microscope, by Nikon (1 mentions) Ab33985, by Abcam (1 mentions) Ab208943, by Abcam (1 mentions) Fitc labeled goat anti rabbit igg, by Beyotime (1 mentions) Lsm 880, by Zeiss (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Abcam (1 mentions) Confocal microscope, by Zeiss (1 mentions)

Close spelling variants of this product

FITC-labeled Goat Anti-Mouse IgG (H + L (5 citations) FITC-labeled goat anti-mouse IgG (H + L) antibody (4 citations) FITC-labeled goat anti-mouse IgG (A0568, (3 citations) Fluorescein isothiocyanate (FITC)-labeled goat anti-mouse IgG (3 citations) FITC-labeled goat anti-mouse (2 citations) FITC goat anti-mouse IgG (2 citations)

15 protocols using fitc labeled goat anti mouse igg

1

Reactive Oxygen Species Sensing Nanoplatform

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Manganese chloride tetrahydrate (MnCl2•4H2O), tetramethylammonium hydroxide solution (TMA, 1.0 M), bovine serum protein (BSA), and 2′,7′-dichloroflourescin diacetate (DCFH-DA) were purchased from Sigma-Aldrich. NH2-PEG3000 and chlorin e6 (Ce6) were purchased from JenKem Technology and Frontier, respectively. The hydrogen peroxide (H2O2, 30 wt.%) solution was purchased from Sinopharm Chemical Reagent Co., Ltd. Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were bought from KeyGen BioTech and Gibco, respectively. HIF-1α antibody (mouse monoclonal antibody) and FITC-labeled goat anti-mouse IgG were purchased from Beyotime Biotechnology. All solutions were prepared by using ultrapure water (18.2 MΩ).
Xiu W., Gan S., Wen Q., Qiu Q., Dai S., Dong H., Li Q., Yuwen L., Weng L., Teng Z., Mou Y, & Wang L. (2020). Biofilm Microenvironment-Responsive Nanotheranostics for Dual-Mode Imaging and Hypoxia-Relief-Enhanced Photodynamic Therapy of Bacterial Infections. Research, 2020, 9426453.
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2

Adrenal Gland Immunohistochemistry Protocol

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Human adrenal glands were fixed in 4% paraformaldehyde at 4℃ overnight, and transferred sequentially to 10%, 20%, and 30% sucrose / PBS. Tissues were embedded by OCT, and placed at −80℃. Tissues were sectioned (Leica CM1950 cryostat) into 20 μm slices and dried at room temperature. Slices were post-fixed in 4% paraformaldehyde for 15 min, washed (cold PBS), incubated in 0.5% PBST for 10 min, and blocked (10% horse serum, 0.3% PBST) for 2 hours at room temperature. Slices were then incubated in primary antibody solution [primary antibody (mouse anti-CaV1.2: 1:200, AB11001554, Neuromab; mouse anti-CaV1.3: 1:200, AB10673964, Neuromab; mouse anti-CaV3.1: 1:200, AB2069421 Neuromab; rabbit anti-CaV3.2: 1:200, #ACC-025, Alomone; rabbit anti-CaV3.3: 1:200, #ACC-009, Alomone; rabbit anti-CYP11B2: 1:200, #ab168388, Abcam), 1% horse serum, and 0.3% PBST] for 1 day at 4℃. Slices were washed with PBS, and incubated in secondary antibody solution [secondary antibody (Cy3-labled goat anti-rabbit IgG or FITC-labeled goat anti-mouse IgG, 1:500, Beyotime), containing 1% horse serum, and 0.3% PBST] at 4℃ overnight. After washing with PBS, slices were treated with DAPI. Confocal images were obtained using a Leica SP2 confocal microscope.
Yang T., He M., Zhang H., Barrett P.Q, & Hu C. (2020). L- and T-type calcium channels control aldosterone production from human adrenals. The Journal of endocrinology, 244(1), 237-247.
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3

Immunofluorescence Staining of Myosin Heavy Chain

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PSCs were fixed in 4% paraformaldehyde and permeabilized with 0.5% Triton X-100 for 30 min, respectively. After three washes, the cells were blocked in QuickBlock™ Blocking Buffer (Beyotime, Shanghai, China) for 2 h, incubated with a primary antibody overnight and incubated with a secondary antibody for 1 h, in turn. Finally, the nuclei were stained with DAPI reagent. Images of at least three random fields of view were captured using a Nikon ECLIPSE Ti microscope (Nikon, Tokyo, Japan) and quantified using ImageJ. The primary antibody was MyHC (sc-376157; Santa Cruz; Delaware, Santa Cruz, CA, USA). The secondary antibody was FITC-labeled Goat Anti-Mouse IgG (A0568; Beyotime, Shanghai, China).
Wang S., Tan B., Xiao L., Zhao X., Zeng J., Hong L., Yang J., Cai G., Zheng E., Wu Z, & Gu T. (2022). Comprehensive Analysis of Long Noncoding RNA Modified by m6A Methylation in Oxidative and Glycolytic Skeletal Muscles. International Journal of Molecular Sciences, 23(9), 4600.
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4

Osteogenic Differentiation Marker Expression

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HAECs, hBMSCs, and hAFMSCs cultured in the osteogenic medium and EXP-CM for 10 days were fixed with 4% paraformaldehyde solution and permeabilized with 0.1% Triton X-100. Nonspecific binding sites were blocked with 5% BSA. After coincubation with primary rabbit polyclonal anti-human OPN antibodies (1 : 100, Proteintech Group, USA) and primary mouse monoclonal anti-human Runx2 antibodies (1 : 100, ProSci Incorporated, USA) over night, the samples were washed and coincubated with Cy3-labeled goat anti-rabbit IgG (1 : 1000, Beyotime, China) and FITC-labeled goat anti-mouse IgG (1 : 1000, Beyotime, China). At the end of the incubation, the samples were counterstained with DAPI (1 : 5000, Beyotime, China). The Cy3, FITC, and DAPI images were taken separately using a fluorescence microscope (DP72; Olympus, Japan) equipped with a digital image capture system (Olympus).
Si J., Dai J., Zhang J., Liu S., Gu J., Shi J., Shen S.G, & Guo L. (2015). Comparative Investigation of Human Amniotic Epithelial Cells and Mesenchymal Stem Cells for Application in Bone Tissue Engineering. Stem Cells International, 2015, 565732.
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5

Immunofluorescence Assay of Viral Infection

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We performed immunofluorescence assay at day 9 postinfection according to previous research on primary hippocam-pal cells.32 (link) Cells were fixed in 4% (w/v) paraformaldehyde for 15 minutes, then treated with 0.25% (v/v) Triton X-100 for 10 minutes, and blocked with 5% (w/v) BSA for 40 minutes. Neurons were incubated with rabbit anti-MAP2 antibody (1:2,000 dilution; Beyotime Biotechnology, Shanghai, China) and mouse anti-P24 monoclonal antibody (1:200 dilution) overnight at 4°C, followed by Cy3-labeled goat anti-rabbit IgG antibody (1:500 dilution; Beyotime Biotechnology) and FITC-labeled goat anti-mouse IgG (1:500 dilution; Beyotime Biotechnology) for 1 hour. Finally, the cells were stained with DAPI (1:5,000 dilution; Beyotime Biotechnology). The cells were imaged using an inverted fluorescence microscope (Nikon, Tokyo, Japan).
Li C., Xu X., Zhang X., Cheng K., Guo Y., Jie J., Guo H., He Y., Zhou C., Gui S., Zhong X., Wang H, & Xie P. (2018). Activation of ERK/CREB/BDNF pathway involved in abnormal behavior of neonatally Borna virus-infected rats. Neuropsychiatric Disease and Treatment, 14, 3121-3132.
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6

Immunofluorescence Staining of Cells

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The cells were fixed in 4% paraformaldehyde for 15 min, and washing with PBS three times. The cells were covered with 0.1%tritonX-100 following incubation for 30 min. After washing with PBS, cells were blocked with goat serum. Subsequently, the primary antibodies (CD31, 1:200, Abcam, Cambridge, UK; FSP1, 1:200, Affinity, Jiangsu, China) were added with cells at 4°C overnight. After clearing the primary antibodies, cells were stained with secondary antibodies (Cy3-labeled goat-anti-rabbit IgG, 1:200, Beyotime, Haimen, China; FITC-labeled goat-anti-mouse IgG, 1:200, Beyotime) for 60 min at room temperature. Following staining with DAPI, cells were observed under a fluorescence microscope.
Yao L., Shao W., Chen Y., Wang S, & Huang D. (2021). Suppression of ADAM8 attenuates angiotensin II-induced cardiac fibrosis and endothelial-mesenchymal transition via inhibiting TGF-β1/Smad2/Smad3 pathways. Experimental Animals, 71(1), 90-99.
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7

Immunofluorescence Analysis of Mitochondria and Lysosomes

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MC3T3-E1 cells were seeded in confocal dishes with different treatments, fixed with paraformaldehyde, and permeabilized with 0.2% Triton-X. After blocking with 5% BSA, cells were incubated with rabbit anti-TOM20 (CST, #42406) or rabbit anti-HSP60 (CST, #4870) overnight at 4 °C. After washing and incubating with FITC-labeled goat anti-rabbit IgG (Beyotime, China) or Cy3-labeled goat anti-rabbit IgG (Beyotime, China). For immunofluorescence co-localization, cells with different treatments were fixed, permeabilized and blocked as mentioned above, and incubated with rabbit anti-LAMP1 (abcam, ab208943) and mouse anti-COX IV (abcam, ab33985) overnight at 4 °C, and then incubated with Cy3-labeled goat anti-rabbit IgG (Beyotime, China) and FITC-labeled goat anti-mouse IgG (Beyotime, China). All of the cells were stained by DAPI (Abcam,Cambridge,UK), and observed by ZEISS LSM 880 (Carl Zeiss, Germany).
Li W., Jiang W.S., Su Y.R., Tu K.W., Zou L., Liao C.R., Wu Q., Wang Z.H., Zhong Z.M., Chen J.T, & Zhu S.Y. (2023). PINK1/Parkin-mediated mitophagy inhibits osteoblast apoptosis induced by advanced oxidation protein products. Cell Death & Disease, 14(2), 88.
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8

Immunofluorescent Localization of MLKL

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Four percent paraformaldehyde‐fixed atria were embedded in OCT compound, snap‐frozen and sliced into 5‐µm‐thick sections using a freezing microtome (Leica, Wetzlar, Germany). Cryosections were fixed and permeabilized with ice‐cold acetone and blocked with 5% BSA at room temperature before incubated with mouse anti‐MLKL antibody (1:200, Millipore, Merck KGaA) at 4℃ overnight. FITC‐labeled goat‐anti‐mouse IgG (1:200, Beyotime, Shanghai, China) was added sequentially, and the nuclei were stained with DAPI. Finally, the slides were mounted with 50% glycerol and observed under confocal microscope (Zeiss, Oberkochen, Germany) for fluorescent signal analysis.
Fu Y., Jiang T., Sun H., Li T., Gao F., Fan B., Li X., Qin X, & Zheng Q. (2021). Necroptosis is required for atrial fibrillation and involved in aerobic exercise‐conferred cardioprotection. Journal of Cellular and Molecular Medicine, 25(17), 8363-8375.
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9

Pneumococcal Binding Assay in A549 Cells

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A549 cells (Type II epithelial lung carcinoma cells, ATCC) were cultured on 24-well plates with Dulbecco’s modified Eagle’s medium (DMEM) accompanied by 10% fetal bovine serum (FBS) without antibiotics to a concentration of 3×105 cells/ml. S. pneumoniae (19F, ZMU3H13628) (5×106 CFU/ml, 0.1 ml) was incubated with the anti-NanAT1-Tuf-PlyD4 antisera for 30 mins. Then, the bacterial suspension was added into the A549 cell culture wells and incubated for 1 h at 37°C. Next, FITC-labeled goat anti-mouse IgG (Beyotime Biotechnology, Shanghai, China) was added to indicate the reacted cells, which were photographed and merged. The antisera from adjuvant-immunized mice were used as controls.
Cui Y., Miao C., Chen W., Shang W., Qi Q., Zhou W., Wang X., Li Y., Yan Z, & Jiang Y. (2022). Construction and protective efficacy of a novel Streptococcus pneumoniae fusion protein vaccine NanAT1-TufT1-PlyD4. Frontiers in Immunology, 13, 1043293.
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10

HN Protein Expression and Detection

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293T cells grown to 80% confluency in 6-well plates were transfected with 2 μg of plasmid (wild-type or mutant HN) diluted in 200 μL jetPRIME buffer (Polyplus-transfection SA) for 4 h at 37°C. The transfection medium was replaced with grown medium and cells were incubated for 24 h. The cells were fixed with 4% paraformaldehyde for 15 min, blocked with 2% bovine serum albumin for 1 h and incubated with HN monoclonal antibody at 4°C overnight. After washing, the cells were incubated by FITC-labeled goat anti-mouse IgG (Beyotime, China) for 30 min, and subsequently incubated with DAPI for 5 min. The fluorescence was observed by an inverted fluorescence microscope (Olympus, Japan). For detecting the binding of sCVPS to HN, the sCVPS-FITC was incubated with transfected cells instead of HN antibody at 4°C for 30 min, and the secondary antibody is no longer used for observation.
Song X., Liu L., Hu W., Liang X., He C., Yin L., Ye G., Zou Y., Li L., Tang H., Jia R, & Yin Z. (2021). Identification of the amino acids residues involved in hemagglutinin-neuraminidase of Newcastle disease virus binding to sulfated Chuanmingshen violaceum polysaccharides. Poultry Science, 100(8), 101255.
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