HEK293FT cells (Thermo Fisher Scientific) were CaCl2-transfected with a plasmid mix containing pLKO.1 lentiviral expressing plasmid, PAX2 and MD2 viral packing plasmids. 48 h post-transfection, virus-containing supernatant from transfected HEK293FT cells was collected, filtered and mixed with polybrene (Sigma-Aldrich) and an additional 10% FBS before applying to Ror2fl/fl/ROSA26LSL-YFP HFSCs. Viral infection was performed by centrifugation of HFSCs with virus-containing mix at 1100 × g at 35 °C for 1 h. 5 days after infection, HFSCs were purified by FACS based on RFP and/or YFP expression. The following lentiviral expressing plasmids were used: pLKO.1-NLS-iCre-mRFP (kind gift from S. Beronja)83 (link), pLKO.1-MCS-mRFP (derived from pLKO.1-NLS-iCre-mRFP where NLS-iCre was replaced by multiple cloning sites).
Hek293ft cells
Manufactured by Merck Group
HEK293FT cells are a human embryonic kidney cell line that is widely used in research and development. These cells are capable of high-level expression of proteins, making them a valuable tool for various applications, such as the production of recombinant proteins, viral vector production, and transfection studies. The HEK293FT cell line is known for its high transfection efficiency and ease of use, making it a popular choice among researchers.
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Lab products found in correlation
2 protocols using hek293ft cells
1
Lentiviral Transduction of HFSCs
2
Maintenance of Podocyte Cell Lines
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Mouse wild type and CD2AP−/− podocytes, HEK 293FT cells (Sigma-Aldrich, St. Louis, MO) and conditionally immortalized human podocytes (AB 8/13) were maintained as decribed42 (link). Media and foetal bovine serum (FBS) were from Sigma-Aldrich, ITS from Gibco (Paisley, UK) and ultraglutamine from Lonza (Basel, Switzerland).
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