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Il 13 ebio13a

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The IL-13 (eBio13A) is a recombinant anti-human IL-13 monoclonal antibody. It is designed for use in immunoassay applications.

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Lab products found in correlation

Facsaria 2, by BD (2 mentions) Fc block, by Thermo Fisher Scientific (2 mentions) Cell stimulation cocktail, by Thermo Fisher Scientific (2 mentions) Dnase, by Merck Group (2 mentions) Fixation and permeabilization buffer, by BioLegend (2 mentions) Dithiothreitol (dtt), by Merck Group (2 mentions) Dispase, by Merck Group (2 mentions) Fixable live dead stain, by Thermo Fisher Scientific (2 mentions) Epcam g8, by BioLegend (2 mentions) Il 22 1h8pwsr, by Thermo Fisher Scientific (2 mentions) Cd45 30 f11, by BioLegend (2 mentions) Fixable viability dye, by Thermo Fisher Scientific (1 mentions) Intracellular fixation permeabilization buffer set, by Thermo Fisher Scientific (1 mentions) Stabilizing fixative, by BD (1 mentions) Rm4 5, by BD (1 mentions) Il 17 tc11 18h10, by BD (1 mentions) Ifn γ xmg1.2, by BioLegend (1 mentions) Ifn γ xmg1.2, by BD (1 mentions) Trfk4, by Thermo Fisher Scientific (1 mentions) Ebio1316h, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

IL-13 (429 citations) EBio13A (24 citations) Anti-IL-13 (eBio13A; (11 citations) Anti-IL-13 (clone eBio13A; (2 citations) IL-13 (clone eBio13A, (2 citations) PE-conjugated anti–IL-13 (eBio13A, (2 citations)

5 protocols using il 13 ebio13a

1

Isolation and Characterization of Colonic Immune Cells

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Lamina propria cells from colonic tissue were harvested as before8 (link). For intracellular cytokine staining, cells were stimulated using the eBioscience cell stimulation cocktail for 4 h at 37 °C. Cells were fixed and permeabilized using the Biolegend fixation and permeabilization buffer. The following antibodies (clones) were used for staining: CD45 (30-F11), CD19 (6D5), CD11b (M1/70), CD90.2 (53–2.1), CD3 (145–2C11), TCR-β (H57–597), CD4 (RM4–5), CD8 (53–6.7), Gr-1 (RB6–8C5), MHCII (M5/114.15.2), CD11c (N418), CD103 (2E7), IFN-γ (XMG1.2) from Biolegend and IL-22 (1H8PWSR), and IL-13 (eBio13A) from eBioscience. All samples were blocked with Fc block (TruStainfcx) and stained with a fixable live dead stain (Invitrogen). FlowJo v.10 was used to analyze flow cytometry data. Intestinal epithelial cells (IECs) were harvested by incubation at 37°C with 2mM DTT (Sigma) followed by two incubations with 5mM EDTA and then digested with Dispase (Sigma) and DNase (Sigma). IECs were sorted as PI−CD45−EpCam+ using the following antibody clones: CD45 (30-F11) and EpCam (G8.8) from Biolegend on an FACSAria II (BD Biosciences). Purity of IECs was ≥ 95%.
Neil J.A., Matsuzawa-Ishimoto Y., Kernbauer-Hölzl E., Schuster S.L., Sota S., Venzon M., Dallari S., Galvao Neto A., Hine A., Hudesman D., Loke P., Nice T.J, & Cadwell K. (2019). IFN-I and IL-22 mediate protective effects of intestinal viral infection. Nature microbiology, 4(10), 1737-1749.
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2

Characterization of CD4+ T Cell Subsets

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Draining cervical lymph nodes were mechanically pressed through a 70-μm filter with PBS. The resulting single cell suspension was resuspended in 0.5% BSA followed by anti-FcR blockade. Cells were immunostained with surface antigen CD4 (RM4-5, BD Biosciences) at 2–4 μg/ml cell suspension for 20 min on ice. The cells were then washed, resuspended in PBS, and stained with Fixable Viability Dye (eBioscience) for 20 min on ice. The following antibodies were used for intracellular staining of the T cells prepared with Intracellular Fixation & Permeabilization Buffer Set (eBioscience): IL-13 (eBio13A, eBioscience), IFN-γ (XMG1.2, BioLegend), and IL-17 (TC11-18H10, BD Biosciences) at 8–20 μg/ml of cell suspension at room temperature for 30 min. Cells were washed and fixed with Stabilizing Fixative (BD). Data were acquired on BD Fortessa LSRII and analyzed with FlowJo (Treestar Inc.)
Singh P.P., Yu C., Mathew R., Perez V.L, & Saban D.R. (2021). Meibomian gland dysfunction is suppressed via selective inhibition of immune responses by topical LFA-1/ICAM antagonism with lifitegrast in the allergic eye disease (AED) model. The ocular surface, 21, 271-278.
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3

Isolation and Characterization of Colonic Immune Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Lamina propria cells from colonic tissue were harvested as before8 (link). For intracellular cytokine staining, cells were stimulated using the eBioscience cell stimulation cocktail for 4 h at 37 °C. Cells were fixed and permeabilized using the Biolegend fixation and permeabilization buffer. The following antibodies (clones) were used for staining: CD45 (30-F11), CD19 (6D5), CD11b (M1/70), CD90.2 (53–2.1), CD3 (145–2C11), TCR-β (H57–597), CD4 (RM4–5), CD8 (53–6.7), Gr-1 (RB6–8C5), MHCII (M5/114.15.2), CD11c (N418), CD103 (2E7), IFN-γ (XMG1.2) from Biolegend and IL-22 (1H8PWSR), and IL-13 (eBio13A) from eBioscience. All samples were blocked with Fc block (TruStainfcx) and stained with a fixable live dead stain (Invitrogen). FlowJo v.10 was used to analyze flow cytometry data. Intestinal epithelial cells (IECs) were harvested by incubation at 37°C with 2mM DTT (Sigma) followed by two incubations with 5mM EDTA and then digested with Dispase (Sigma) and DNase (Sigma). IECs were sorted as PI−CD45−EpCam+ using the following antibody clones: CD45 (30-F11) and EpCam (G8.8) from Biolegend on an FACSAria II (BD Biosciences). Purity of IECs was ≥ 95%.
Neil J.A., Matsuzawa-Ishimoto Y., Kernbauer-Hölzl E., Schuster S.L., Sota S., Venzon M., Dallari S., Galvao Neto A., Hine A., Hudesman D., Loke P., Nice T.J, & Cadwell K. (2019). IFN-I and IL-22 mediate protective effects of intestinal viral infection. Nature microbiology, 4(10), 1737-1749.
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4

Comprehensive Immune Cell Profiling

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Lymph nodes, BAL, spleen and lungs were harvested and single-cell suspensions were prepared. For intracellular staining, cells were stimulated with 1μM ionomycin and 10ng/ml PMA for 4–6h and 5μM of monensin. Cell were stained with live/dead (In vitrogen), CD4 (RM4.5), CD44 (IM7), CD45.1 (A20), CD45.2 (104), IL-2 (JES6-5H4), IL-4 11B11), IL-5 (TRFK5), IL-17A (TC11-18H10), IFN-γ (XMG1.2) (BD Bioscience), IL-13 eBio13A) (eBioscience) antibodies. IL-1R1 (JAMA 174) antibody was purchased from BioLegend, and stained using Faser Kit-PE amplification system (Miltenyi). Data were collected with BDLSRII and analyzed with FlowJo (TreeStar).
Caucheteux S.M., Hu-Li J., Guo L., Bhattacharyya N., Crank M., Collins M.T, & Paul W.E. (2016). Interleukin-1 beta enhances inflammatory Th2 differentiation. The Journal of allergy and clinical immunology, 138(3), 898-901.e4.
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5

Cytokine Levels Quantification via ELISA

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Cytokine levels were detected in culture supernatants and BAL fluid by ELISA using monoclonal capture and biotinylated detection antibody pairs as follows, used at concentrations optimised previously: IL-4 (11B11 + BVD6-24G2 (BD Pharmingen)); IL-5 (TRFK5 + TRFK4 (eBioscience)); IL-13 (eBio13A + eBio1316H (eBioscience). p-nitrophenyl phosphate (pNPP, 1 mg/ml, Sigma) was used as a substrate. OD was measured at 405 nm on a Precision microplate reader (Molecular Devices) and data analysed using Softmax Pro software.
Varyani F., Löser S., Filbey K.J., Harcus Y., Drurey C., Poveda M.C., Rasid O., White M.P., Smyth D.J., Gerbe F., Jay P, & Maizels R.M. (2022). The IL-25-dependent tuft cell circuit driven by intestinal helminths requires macrophage migration inhibitory factor (MIF). Mucosal Immunology, 15(6), 1243-1256.
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