The breast MCF10A epithelial cell line, the BC cell lines (MDA231, MCF7, MDA468 and BT474) and 293T cell line were purchased from BeNa Culture Collection (Beijing, China). The cell lines were free from mycoplasma contamination. The MDA231, MCF7, MDA468 and BT474 cell lines were cultured in DMEM supplemented with 10% FBS and 100 U/ml penicillin and streptomycin (all from Gibco; Thermo Fisher Scientific, Inc.). The MCF10A cell line was maintained in RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS (Gibco; Thermo Fisher Scientific, Inc.) and 1% penicillin and streptomycin (ScienCell Research Laboratories, Inc.). The cell lines were maintained at 37°C in a humidified atmosphere with 5% CO2. In addition, hypoxia treatment comprised 94% N2, 5% CO2 and 1% O2 at 37°C for different durations under hypoxic conditions. Hypoxia was simulated by culturing cells with CoCl2 (Sigma-Aldrich) at a final concentration of 100 µM.
293t cell line
Manufactured by BNCC
Sourced in China
The 293T cell line is a widely used human embryonic kidney cell line. It is a derivative of the 293 cell line and is commonly used in cell and molecular biology research as a versatile and robust expression system. The 293T cell line is known for its high transfection efficiency, which makes it a valuable tool for the production of recombinant proteins, viral vector generation, and various other applications in the field of biotechnology and life sciences.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Mcf 10a, by Thermo Fisher Scientific (1 mentions)
Bt474, by Thermo Fisher Scientific (1 mentions)
Mda 231, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Mcf 7, by Thermo Fisher Scientific (1 mentions)
Cocl2, by Merck Group (1 mentions)
Mcf 7, by BNCC (1 mentions)
Bt474, by BNCC (1 mentions)
Penicillin, by ScienCell (1 mentions)
Streptomycin, by ScienCell (1 mentions)
Hemecs, by BNCC (1 mentions)
Hemecs, by Thermo Fisher Scientific (1 mentions)
Cd90 fitc, by BD (1 mentions)
Influx cell sorter, by BD (1 mentions)
Sn12c, by BNCC (1 mentions)
4 protocols using 293t cell line
1
Breast Cancer Cell Line Culturing and Hypoxia Assay
2
Isolation and Culture of Primary Hemangioma Cells
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Human primary hemangioma endothelial cells (HemECs) and the 293T cell line were purchased from the BeNa Culture Collection. HemECs were maintained in complete endothelial cell medium (Gibco; Thermo Fisher Scientific, Inc.), whereas the 293T cells were maintained in DMEM (Gibco; Thermo Fisher Scientific, Inc.) containing 10% FBS (Gibco; Thermo Fisher Scientific, Inc.). All cells were cultured in a humidified incubator with 5% CO2 at 37˚C. Ethics approval for the use of HemECs was waived by the Ethics Committee of the Affiliated Hospital of Hebei University of Engineering (Handan, China).
3
MSC Surface Marker Identification
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The human BMSCs (hBMSC) and the 293 T cell line were all purchased from BeNa Culture Collection (Beijing, China), maintained in DMEM (Dulbecco's modified Eagle’s medium) with 10% fetal bovine serum (FBS), 4 mM L-glutamine, and sodium pyruvate, and incubated in a 5% CO2 atmosphere at 37 °C. Then the cells were digested and incubated with specific antibodies for identification of surface markers. To verify the MSC, we selected human CD44-FITC and CD90 FITC (all from BD, San Jose, CA) as the antibodies for surface markers. A BD Influx cell sorter (BD) was used to perform flow cytometry.
4
Renal Cell Carcinoma Cell Lines Protocol
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RCC cell lines, including A498 (cat. no. BNCC338630; BeNa Culture Collection), A704 (cat. no. BNCC342393; BeNa Culture Collection), SN12C (cat. no. BNCC341858; BeNa Culture Collection), 769-P (cat. no. BNCC101643; BeNa Culture Collection), the human renal fibroblast cell line KFB (cat. no. HTX2520; Otwo Biotech), human proximal convoluted tubular cell line HK-2 (cat. no. BNCC338012; BeNa Culture Collection) and the 293T cell line (cat. no. MZ-0005; Ningbo Mingzhou Biotechnology Co., Ltd.) were used. Briefly, the cells were seeded in a 10-mm culture dish and cultivated in DMEM (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS (Gibco; Thermo Fisher Scientific, Inc.) and 1% penicillin and streptomycin in a humidified incubator containing 5% CO2 and 95% air at 37°C. The culture medium was replaced every 2 days. Cell passage was conducted using trypsin at over 80% of confluence.
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