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Low attachment 6 well plates

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Low-attachment 6-well plates are a type of laboratory equipment designed to promote the formation of 3D cell cultures. They feature a non-adherent surface that encourages cells to aggregate and form spheroids or organoids, which can be useful for various cell-based assays and research applications.

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Lab products found in correlation

Fibroblast growth factor (fgf), by Thermo Fisher Scientific (3 mentions) Hank s balanced salt solution, by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Dmem f12 media, by Thermo Fisher Scientific (2 mentions) Epidermal growth factor (egf), by Thermo Fisher Scientific (2 mentions) Mk 2206, by Selleck Chemicals (1 mentions)

Spelling variants of this product

6-well plates (338 citations) Ultra-low attachment 6-well plates (6 citations) Ultra Low Attachment 6-well culture plates (3 citations)

5 protocols using low attachment 6 well plates

1

Fetal Cardiac Tissue Characterization

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Fetal hearts were purchased as surgical waste from Advanced Bioscience Resources (Alameda, CA), and delivered on ice within 2.5 hours of surgery. Left ventricles were sectioned from the apex towards the atria into 7mm long x 2mm wide strips, washed 3 times in Hanks Balanced Salt Solution (Gibco), transferred to low attachment 6-well plates (Nunc) containing RPMI+B27 media, and placed into the incubator for 1 hour before taking measurements. FCT strips were analyzed in a similar manner to the cardiac tissues for contractile behavior, force generation, gene expression, cardiac proteins, ultrastructure and histomorphology, as detailed below. In addition, RNA isolated from 32 pooled fetal hearts, ages 21–37 weeks, was obtained from Clontech (Mountain View, CA) for gene expression studies.
Ronaldson-Bouchard K., Ma S.P., Yeager K., Chen T., Song L., Sirabella D., Morikawa K., Teles D., Yazawa M, & Vunjak-Novakovic G. (2018). Advanced maturation of human cardiac tissue grown from pluripotent stem cells. Nature, 556(7700), 239-243.
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2

Fetal Cardiac Tissue Characterization

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Fetal hearts were purchased as surgical waste from Advanced Bioscience Resources (Alameda, CA), and delivered on ice within 2.5 hours of surgery. Left ventricles were sectioned from the apex towards the atria into 7mm long x 2mm wide strips, washed 3 times in Hanks Balanced Salt Solution (Gibco), transferred to low attachment 6-well plates (Nunc) containing RPMI+B27 media, and placed into the incubator for 1 hour before taking measurements. FCT strips were analyzed in a similar manner to the cardiac tissues for contractile behavior, force generation, gene expression, cardiac proteins, ultrastructure and histomorphology, as detailed below. In addition, RNA isolated from 32 pooled fetal hearts, ages 21–37 weeks, was obtained from Clontech (Mountain View, CA) for gene expression studies.
Ronaldson-Bouchard K., Ma S.P., Yeager K., Chen T., Song L., Sirabella D., Morikawa K., Teles D., Yazawa M, & Vunjak-Novakovic G. (2018). Advanced maturation of human cardiac tissue grown from pluripotent stem cells. Nature, 556(7700), 239-243.
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3

3D Mammosphere Culture Protocol

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Mammospheres were cultured in low-attachment (also referred to as 3D culture) in DMEM/F-12 media supplemented with B-27, penicillin-streptomycin, fibroblast growth factor (FGF) and epidermal growth factor (EGF) (Invitrogen, Carlsbad, CA) at 37 °C in humidified 5% CO2. Mammospheres were created by plating TU-BCx-2 K1 PDX cells (50,000 cells) in low suspension DMEM/F-12 media supplemented with fibroblast and epidermal growth factors (20 ng/mL each; Miltenyi Biotec, Bergisch Gladbach, Germany) in low-attachment 6-well plates (ThermoFisher Scientific, Waltham MA). Growth factors were added to the spheres every 3 days. Sphere growth was observed with brightfield microscopy and representative images were captured every 3 days.
Matossian M.D., Burks H.E., Elliott S., Hoang V.T., Bowles A.C., Sabol R.A., Wahba B., Anbalagan M., Rowan B., Abazeed M.E., Bunnell B.A., Moroz K., Miele L., Rhodes L.V., Jones S.D., Martin E.C., Collins-Burow B.M, & Burow M.E. (2019). Drug resistance profiling of a new triple negative breast cancer patient-derived xenograft model. BMC Cancer, 19, 205.
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4

Mammosphere Culture and Inhibitor Treatment

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Mammospheres were cultured in low suspension media composed of DMEM/F-12 media supplemented with B-27, penicillin-streptomycin, fibroblast growth factor (FGF) and epidermal growth factor (EGF) (Invitrogen, Carlsbad, CA). Mammospheres were created by plating TU-BCx-2K1 PDX cells (50,000) in low suspension DMEM/F-12 media supplemented with FGF and EGF (20 ng/mL each) in low-attachment 6-well plates (Thermo Fisher Scientific, Waltham MA). Mammospheres were treated with GSI PF-03084014, AKT inhibitor (MK-2206) or IKK inhibitor, Bay11-70820 (Selleckchem, Texas) as described earlier.
Hossain F., Sorrentino C., Ucar D.A., Peng Y., Matossian M., Wyczechowska D., Crabtree J., Zabaleta J., Morello S., Del Valle L., Burow M., Collins-Burow B., Pannuti A., Minter L.M., Golde T.E., Osborne B.A, & Miele L. (2018). Notch Signaling Regulates Mitochondrial Metabolism and NF-κB Activity in Triple-Negative Breast Cancer Cells via IKKα-Dependent Non-canonical Pathways. Frontiers in Oncology, 8, 575.
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5

3D Mammosphere Culture Protocol

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Mammospheres were cultured by plating explants in 3D culture composed of DMEM/F-12 media supplemented with B-27, penicillin- streptomycin, fibroblast-growth factor and epidermal-growth factor (Invitrogen, Carlsbad, CA, USA). Explants were plated in low-attachment 6-well plates (ThermoFisher Scientific, Waltham MA, USA) and primary spheres grew for up to 10 days before harvesting and quantified. Sphere area was quantified in the Aperio program.
Matossian M.D., Burks H.E., Elliott S., Hoang V.T., Bowles A.C., Sabol R.A., Bunnell B.A., Martin E.C., Burow M.E, & Collins-Burow B.M. (2018). Panobinostat suppresses the mesenchymal phenotype in a novel claudin-low triple negative patient-derived breast cancer model. Oncoscience, 5(3-4), 99-108.
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