Photomultiplier

Manufactured by Hamamatsu Photonics

Sourced in Japan

A photomultiplier is an electronic device that converts light into an electrical signal. It consists of a photocathode that generates electrons when exposed to light, and a series of dynodes that amplify the signal. The photomultiplier is designed to detect and measure low-level light signals with high sensitivity and speed.

Automatically generated - may contain errors

Lab products found in correlation

Plp 10, by Hamamatsu Photonics (2 mentions) Fluorolog 3, by Horiba (2 mentions) Lsm 700 confocal microscope, by Zeiss (2 mentions) Fs5 spectrofluorometer, by Edinburgh Instruments (1 mentions) Orca flash4.0 v2, by Hamamatsu Photonics (1 mentions) Volocity, by PerkinElmer (1 mentions) Fs5 spectrofluorometer, by Hamamatsu Photonics (1 mentions) K alpha model, by Thermo Fisher Scientific (1 mentions) Jsm 7800f, by JEOL (1 mentions) Spectrofluorimeter, by Horiba (1 mentions) Fluorolog spectrofluorimeter, by Horiba (1 mentions) C1 microscope, by Nikon (1 mentions) Ihr320, by Horiba (1 mentions) Mplan10 0, by Olympus (1 mentions) T64000 triple spectrometer, by Horiba (1 mentions) Peltier cooled charge coupleddevice detector, by Horiba (1 mentions) Thms600, by Linkam (1 mentions) Newton emccd camera, by Oxford Instruments (1 mentions)

13 protocols using photomultiplier

Adsorption and Photoluminescence of JUK-20-x

Check if the same lab product or an alternative is used in the 5 most similar protocols

The
adsorption measurements for N₂, CO₂, water (H₂O and D₂O), and alcohol vapors were performed at
77, 273–293, 293, and 300 K, respectively. The virtual porosity
of JUK-20-x was analyzed using Zeo++ calculations of the framework
models based on the crystal structures (Table S1).^{44 (link)} The carbon dioxide heat of
adsorption was determined by fitting a Freundlich model for carbon
dioxide adsorption isotherms at three temperatures: 273, 283, and
293 K. Solid-state photoluminescent characterization for all reported
compounds was performed using an FS5 spectrofluorometer (Edinburgh
Instruments) equipped with a Xe arc lamp (150 W, excitation spectra)
serving as an excitation source and a Hamamatsu photomultiplier of
the R928P type as a detector. Humidity-dependent measurements were
performed in situ using a home-made setup employing HG-100 RH humidity
generator (L&C Science and Technology).

Jędrzejowski D., Ryndak M., Zakrzewski J.J., Hodorowicz M., Chorazy S, & Matoga D. (2023). Covalent Modification by Click Mechanochemistry: Systematic Installation of Pendant OH Groups in a MOF for Rigidity Control and Luminescence-Based Water Detection. ACS Applied Materials & Interfaces, 15(21), 25661-25670.

+ Open protocol

+ Expand

Microscopy Protocols for Tissue and Cell Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols

Images were acquired at room temperature using a Leica DM6 upright microscope (tissues), or a Zeiss LSM700 confocal microscope (tissues and single cells). For the LSM700, excitation lasers were 405 nM (DAPI and BV-421), 488 nM (FITC and Alexa488), 543 nM (R-PE and Alexa546), and 633 nM (Alexa680), with either 40×/1.3 or 63×/1.4 oil immersion objectives and collected using a Hamamatsu photomultiplier. Signal quantifications were done using Volocity (Perkin Elmer). For the DM6 microscope, the filter cubes used were DAPI, YFP, Cy3 and Cy5, with 20× objectives and collected using ORCAflash 4.0 V.2 from Hamamatsu, a high-resolution monochromatic camera. For each experiment, multiple fields were analyzed, excluding cells with saturated signal, abnormal DNA structure or mitotic figures. For figures, images were transferred to Photoshop for cropping, adjusting brightness and contrast for the whole image when necessary to enhance visibility.

Litzler L.C., Zahn A., Dionne K.L., Sprumont A., Ferreira S.R., Slattery M.R., Methot S.P., Patenaude A.M., Hébert S., Kabir N., Subramani P.G., Jung S., Richard S., Kleinman C.L, & Di Noia J.M. (2023). Protein arginine methyltransferase 1 regulates B cell fate after positive selection in the germinal center in mice. The Journal of Experimental Medicine, 220(9), e20220381.

+ Open protocol

+ Expand

Millifluidic Lineage Tracking Assay

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The analysis with the millifluidic lineage tracking machine was performed after dilution in fresh LB medium (Sigma-Aldrich) to obtain, an average number of cell per drop λ ≈ 0.5. Drops were incubated and measured at 37°C as described in [12 (link), 13 (link)]. The fluorescence was measured using a photomultiplier (Hamamatsu) and a YFP filter set (479/40nm excitation and 530/40nm emission, Thorlabs) and an LED source (490nm, Thorlabs). In order to compare experiments, the data were normalized according to a reference measurement on the ancestral population.

Cottinet D., Condamine F., Bremond N., Griffiths A.D., Rainey P.B., de Visser J.A., Baudry J, & Bibette J. (2016). Lineage Tracking for Probing Heritable Phenotypes at Single-Cell Resolution. PLoS ONE, 11(4), e0152395.

+ Open protocol

+ Expand

Magnetic and Spectroscopic Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Alternate-current (ac) and direct-current (dc) magnetic measurements were performed using a Quantum Design MPMS 3 SQUID magnetometer with an Evercool system. Samples during the measurements were placed in the hermetic FEP capsules under an Ar atmosphere. Excitation and emission spectra were recorded on the Edinburgh Instruments FS5 spectrofluorometer equipped with a Xe arc lamp as an excitation source and a Hamamatsu photomultiplier as a detector. Computational details regarding the ab initio calculations of CASSCF/RASSI/SINGLE_ANISO type (together with the related references) performed on the experimental crystal structures of 1–4 were discussed in the Supporting Information.

Wegner W., Zakrzewski J.J., Zychowicz M, & Chorazy S. (2021). Incorporation of expanded organic cations in dysprosium(III) borohydrides for achieving luminescent molecular nanomagnets. Scientific Reports, 11, 11354.

+ Open protocol

+ Expand

Photocatalyst Photoluminescence Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

PL measurements were performed by sampling the same surface areas of the photocatalyst and reference as well (1 cm²), at room temperature by an He–Cd laser (excitation wavelength of 325 nm). PL signals have been recorded by a monochromator and a water-cooled Hamamatsu photomultiplier.

Ussia M., Urso M., Miritello M., Bruno E., Curcuruto G., Vitalini D., Condorelli G.G., Cantarella M., Privitera V, & Carroccio S.C. (2019). Hybrid nickel-free graphene/porphyrin rings for photodegradation of emerging pollutants in water. RSC Advances, 9(52), 30182-30194.

+ Open protocol

+ Expand

Temperature-Dependent Optical Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

The PL and TR-PL measurements were performed as a function of the sample’s temperature in the range of 10–310 K in two positions on the film by exciting the sample with a solid-state pulsed laser (mod. PLP-10, Hamamatsu), which provided pulses at a wavelength of 400 nm for about 58 ps at a repetition rate of 1 MHz. The PL was dispersed with an iHR320 (focal length of 0.32 m) Horiba monochromator equipped with a Peltier cooled Hamamatsu photomultiplier (Head-on mod. R943-02), which operated in the single-photon-counting mode. The time-correlated single-photon-counting (TCSPC) technique was used to record the TR-PL with an Edinburg Instruments TCC900 TSCPC electronics card. The temporal resolution of the system was about 0.46 ns.

Anni M., Cretí A., De Giorgi M.L, & Lomascolo M. (2021). Local Morphology Effects on the Photoluminescence Properties of Thin CsPbBr3 Nanocrystal Films. Nanomaterials, 11(6), 1470.

+ Open protocol

+ Expand

Structural and Optical Characterization of Thin Films

Check if the same lab product or an alternative is used in the 5 most similar protocols

The distinct film thicknesses were measured using a profilometer (Dektak, Bruker, MA, USA). Their structural characteristics were analyzed using X-ray diffraction (XRD; INEL EQUINOX 2000) with Cu-Kα radiation (wavelength, λ = 0.15406 nm). The chemical composition and sample features were evaluated using X-ray photoelectron spectroscopy (XPS; K-Alpha model, Thermo Fisher Scientific, Waltham, MA, USA) with an Al–Kα monochrome X-ray source (for calibration, the C1s peak was used as the reference). The film’s optical properties were characterized via photoluminescence (PL) measurements (Hamamatsu photomultiplier attached to a SPEX spectrometer with a focal length of 50 cm); the film’s PL spectra were recorded with the 325 nm emission line of a 200 mW He–Cd laser. The surface morphologies of the produced films were examined using field-emission scanning electron microscopy (FESEM; JSM-7800F, JEOL, Tokyo, Japan) with an acceleration voltage of 3–5 kV. All of the characterizations were carried out at room temperature.

Hernández-Rodríguez Y.M., Lopez-Salazar P., Juarez-Diaz G., Paredes-Rubio G.R, & Peña-Sierra R. (2022). Synthesis and Characterization of Nanoporous ZnO Films by Controlling the Zn Sublimation by Using ZnO/Zn Precursor Films. Materials, 15(16), 5509.

+ Open protocol

+ Expand

Luminescence Spectra of Thin Films

Check if the same lab product or an alternative is used in the 5 most similar protocols

The luminescence spectra of films obtained by drop-casting from acetonitrile solutions on quartz slides were recorded at room temperature using a Fluorolog-3, Horiba JobinYvon spectrofluorimeter equipped with double-grating monochromator in both the excitation and emission sides. A 450 W Xe arc lamp and an R928P Hamamatsu photomultiplier were employed as excitation source and detector, respectively. The emission spectra were corrected for detection and optical spectral response of the spectrofluorimeter supplied by the manufacturer.

Longhi A., Baron M., Rancan M., Bottaro G., Armelao L., Sgarbossa P, & Tubaro C. (2019). Possible Synthetic Approaches for Heterobimetallic Complexes by Using nNHC/tzNHC Heteroditopic Carbene Ligands. Molecules, 24(12), 2305.

+ Open protocol

+ Expand

Optical Properties of Y₂O₃ and Y₂O₃:Bi Thin Films

Check if the same lab product or an alternative is used in the 5 most similar protocols

The optical properties of all the annealed undoped Y₂O₃ and Y₂O₃:Bi thin films have been analyzed by photoluminescence (PL) and photoluminescence excitation (PLE) spectroscopy at room temperature (RT), performed by a Horiba Fluorolog spectrofluorimeter equipped with a 400 W Xe lamp. The excitation wavelength was varied between 300 nm and 550 nm with a scan step of 1 nm, while the luminescence was detected by a Hamamatsu Photomultiplier.
Cathodoluminescence (CL) properties have been investigated using a commercial Oxford CL system, fitted onto a Cambridge S360 standard tungsten gun scanning electron microscope. The CL system is equipped with a 1800 line/mm grating and a multialkali Photomultiplier sensitive in the range 350–830 nm. The spectroscopic CL analyses are carried out with an accelerating voltage of 3 kV at room temperature and at low temperature (liquid nitrogen, 77 K). The spectral resolution is 5 nm.

Scarangella A., Fabbri F., Reitano R., Rossi F., Priolo F, & Miritello M. (2017). Visible emission from bismuth-doped yttrium oxide thin films for lighting and display applications. Scientific Reports, 7, 17325.

+ Open protocol

+ Expand

Time-resolved Photoluminescence Measurements

Check if the same lab product or an alternative is used in the 5 most similar protocols

The time-resolved PL (TR-PL) measurements were performed by exciting the samples by a solid-state pulsed laser (mod. PLP-10, Hamamatsu), which provides pulse at a wavelength of 400 nm of about 58 ps at a repetition rate of 1 MHz. The PL has been dispersed using an iHR320 (focal length of 0.32 m) Horiba monochromator equipped with a Peltier cooled Hamamatsu photomultiplier (head-on mod R943-02), operating in single-photon counting. The time-resolved PL measurements have been performed by Time Correlated Single Photon Counting (TCSPC) technique using an Edinburg Instruments TCC900 TSCPC electronics card. The temporal resolution of the system is about 0.46 ns. The sample temperature has been changed by a closed circle He cryostat, allowing to change the temperature in the range 10–310 K. The fluorescence microscopy measurements have been performed with a Nikon C1 microscope, exciting the sample with a Hg lamp in the spectral range 340–380 nm, and collecting the F8BT and the NCs PL with band pass filter in the 515–555 nm and 580–630 nm range, respectively.

Balena A., Cretí A., Lomascolo M, & Anni M. (2021). Investigation of the exciton relaxation processes in poly(9,9-dioctylfluorene-co-benzothiadiazole):CsPbI1.5Br1.5 nanocrystal hybrid polymer–perovskite nanocrystal blend. RSC Advances, 11(53), 33531-33539.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!