The crystallographic study of the manufactured nanocomposite was condcuted by using a D8 Advance X-ray diffractometer (Bruker AXS) using monochromatic Cu Kα rays having 2 θ speed of scan per minute. The dimensions (size) and surficial study was measured and analyzed by SEM (Tescan Vega 3 LMU SEM). The absorbance of the bacterial cultures was observed using a nanodrop Thermo 2000 °C spectrophotometer (Thermo Fisher Scientific, USA) with a peak analyzer system. A Platos R 496 microplate reader (Ameda Labor diagnostik GmbH, Graz, Austria) was used to measure the absorbance of the MTT-treated cell line at 550 nm.
Vega3 lmu sem
Manufactured by TESCAN
Sourced in Czechia
The TESCAN Vega3 LMU is a scanning electron microscope (SEM) designed for a wide range of applications. Its core function is to produce high-quality, high-resolution images of samples by scanning them with a focused electron beam. The Vega3 LMU is equipped with advanced features and capabilities to enable comprehensive analysis and characterization of a variety of materials and specimens.
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Lab products found in correlation
6 protocols using vega3 lmu sem
1
Nanocomposite Characterization Techniques
2
Biofilm Visualization and Analysis by SEM
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Scanning electron microscopy (SEM) was used to visualise the appearance and coverage of biofilm developed on the PWG coupons inserts (Figure 1 ). The inner surface (insert) of 3 PWG coupons (total area 90 mm2) was collected at the end of the experiment (day 28). Coupons inserts were fixed overnight with 5% Glutaraldehyde (Glutaraldehyde, 25% aqueous solution, Thermo Fisher United Kingdom) to preserve them until further analysis. Following this, inserts were fixed in 2% aqueous osmium tetroxide for 1 h at room temperature. A series of ethanol dilutions in distilled water were used for dehydrating the inserts in 15-min steps as follows: 75%, 95%, two steps of 100% ethanol, and 100% over anhydrous copper sulphate. The inserts were immersed in a 50/50% (v/v) solution of absolute ethanol and hexamethyldisilazane for 30 min and then transferred to 100% hexamethyldisilazane for a further 30 min. Samples were mounted onto a pin-stub using a Leit-C sticky tab and Leit-C plast, gold coated using an Edwards S150B sputter coater and examined in using a Tescan Vega3 LMU SEM at The University of Sheffield (UoS).
3
SEM Analysis of Polyethylene Surfaces
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Prior to carrying out the SEM analysis, the samples were gold plated in a sputter coater as the samples are non-conductive in nature. The samples were then placed in the chamber of the TESCAN VEGA3 LMU SEM assembly. Backscattered electron images were collected to observe the morphology of the polyethylene surfaces. The LDPE surface was captured at a magnification of 1000x, 1500x and 2000x magnification.
4
Isolation and Characterization of Sugarcane Rhizosphere Fungi
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All soil samples were collected from the 5–15 cm deep sugarcane rhizosphere by five sampling methods in Guangxi province, China. Fungal isolations were obtained by using Chinese cabbage as a baiting plant, as described by Narisawa et al. (1998) (link). Cultural characteristics were recorded after two weeks from potato dextrose agar (PDA ). Conidiophores, conidiogenous cells, and conidia were examined as slide fungal preparations mounted in PVLG (polyvinyl alcohol, Lactic acid, Glycerin, and MiliQ water). Observations and measurements were made with Olympus BX53 Ci-L light microscope. Scanning electron microscopy (SEM ) used a Tescan-vega3 LMU SEM .
5
Visualizing Chitosan Bead Modifications
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The surface of the freeze-dried chitosan beads modified by immobilization of CDH isolated from the four fungal strains, lactose, and cellobiose was visualized using the scanning microscopy technique (SEM) at 30 kV accelerating voltage. Beads containing chitosan with glutaraldehyde (ChGA), ChGA with immobilized lactose, and ChGA with bounded cellobiose were the controls. All of the samples were coated with gold (Sigma-Aldrich, St. Louis, MO, USA) and their morphologies were observed using VEGA-3 LMU SEM (TESCAN, Brno, Czech Republic). The magnification ×10,000 was used to examine the surface of the control and the CDH-, laccase-, and cellobiose-modified samples. In order to check and confirm possible changes in the image of the samples, 10 separate areas with chitosan beads were selected.
6
SEM Visualization of Biofilm Formation
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On day 28, a lead coupon and a piece of PVC tubing were taken from the high phosphate and low phosphate experimental bioreactors for visualization with SEM by the Electron Microscopy Facility of the Faculty of Science of the University of Sheffield. The coupons were fixed for 24 h in 2.5% Glutaraldehyde (Glutaraldehyde, 25% aqueous solution, Thermo Fisher, UK) and washed in PBS and post fixed using 2% aqueous Osmium tetroxide, washed briefly in water and dehydrated. The dehydration consisted of a series of treatments with graded ethanol, samples were dried in a 50/50 mixture of 100% ethanol and hexamethyldisilazane (HEX) with a final drying step in 100% HEX. Samples were mounted onto a pin-stub using a Leit-C sticky tab and Leit-C plast, gold-coated using an Edwards S150B sputter coater and examined in using a Tescan Vega3 LMU SEM.
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