Tgfbri

Manufactured by Cell Signaling Technology

TGFBRI is a lab equipment product that functions as a transforming growth factor beta receptor type I. It is a protein involved in the signaling pathway of transforming growth factor beta, which plays a crucial role in various cellular processes.

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Lab products found in correlation

4 protocols using tgfbri

Analyzing Epithelial-Mesenchymal Transition

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Western blotting was performed as described previously 38 (link). Antibodies against E-cadherin, vimentin, fibronectin, ZEB1, TGFBRI, p SMAD3 and SMAD3 were purchased from Cell Signaling Technology, p65 from Proteintech and p84 from Invitroge. The membranes were stripped and reprobed with an anti-α-tubulin antibody (Sigma-Aldrich, USA) as the loading control.

Dai Y., Wu Z., Lang C., Zhang X., He S., Yang Q., Guo W., Lai Y., Du H., Peng X, & Ren D. (2019). Copy number gain of ZEB1 mediates a double-negative feedback loop with miR-33a-5p that regulates EMT and bone metastasis of prostate cancer dependent on TGF-β signaling. Theranostics, 9(21), 6063-6079.

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Kinase Inhibitors for TGF-beta Signaling

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Antibodies to phosphorylated (pSer^465/467) or total SMAD2, TGFBRI, TGFBRII, pSer⁶³ c-JUN, pSer⁴⁷³ or total AKT, pThr¹⁸⁰/Tyr¹⁸² or total p38MAPK, and GAPDH were all from Cell Signaling Technology. Antibody against SMAD3 was from Invitrogen; antibodies to TGFBR1 (H100) and TGFBRII (L21) were from Santa Cruz; antibodies to Myc-tag and firefly luciferase were from Millipore; antibodies to N-terminal of BUB1 were from Abcam (rabbit) or Santa Cruz (goat); antibody to Flag-HRP was from Sigma; and antibodies to His-HRP were from Invitrogen (clones H3 and C-term) or Millipore (H8 clone). HRP-conjugated and fluorophore-conjugated secondary antibodies were from Jackson ImmunoResearch. Recombinant human TGFβ1 was obtained from HumanZyme. TGFBRI inhibitor SB431542 was obtained from Cayman Chemical. D-Luciferin was from Xenogen Corp. The siGENOME Smart Pool siRNA library targeted against all human kinases, non-silencing siRNA (NSS), and individual siRNA from the siGENOME Smart Pool, and custom siRNA against BUB1 were obtained from Dharmacon. A specific, small molecule inhibitor of BUB1 kinase activity (2-[(4-amino-1-(tert-butyl)-1H-pyrazolo[3,4-d]pyrimidin-3-yl)methyl]phenol; (2OH-BNPP1) (9 (link)) was synthesized in house.

Nyati S., Schinske-Sebolt K., Pitchiaya S., Chekhovskiy K., Chator A., Chaudhry N., Dosch J., Van Dort M.E., Varambally S., Kumar-Sinha C., Nyati M.K., Ray D., Walter N.G., Yu H., Ross B.D, & Rehemtulla A. (2015). The kinase activity of the Ser/Thr kinase BUB1 promotes TGF-β signaling. Science signaling, 8(358), ra1.

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Analysis of TGF-β Signaling Pathway

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Nuclear and/or cytoplasmic fractionation was separated using the Cell Fractionation Kit (Cell Signaling Technology, USA) according to the manufacturer’s instructions, and the whole-cell lysates were extracted with radio-immunoprecipitation assay (RIPA) Buffer (Cell Signaling Technology). Western blotting was performed according to a standard method, as described previously.⁵³ (link) Antibodies against SMAD2, SMAD4, TGFBRI, TGFBRII, p SMAD3, and SMAD3 were purchased from Cell Signaling Technology, and p84 from Invitrogen. The membranes were stripped and reprobed with an anti-α-tubulin antibody (Sigma-Aldrich, USA) as the loading control.

Huang S., Zou C., Tang Y., Wa Q., Peng X., Chen X., Yang C., Ren D., Huang Y., Liao Z., Huang S., Zou X, & Pan J. (2019). miR-582-3p and miR-582-5p Suppress Prostate Cancer Metastasis to Bone by Repressing TGF-β Signaling. Molecular Therapy. Nucleic Acids, 16, 91-104.

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Investigating Cellular Signaling Pathways with Targeted Protein Modulation

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Human recombinant TGF-β1, PDGF and BMP4 were purchased from Biolegend (San Diego, CA). Fast-start universal SYBR Green master mix was from Roche, and sb431542, MG132 and chloroquine were from Sigma. The antibodies used for immunoblotting (IB): GAPDH 1:10000 (Sigma), β-actin 1:5000 (Santa Cruz),Smad2 1:1000 (Cell Signalling), Smad3 1:1000 (Cell Signalling), p-Smad2 (Ser465/467) 1:1000 (Cell Signalling), p-Smad3 (Ser423/425) 1:500 (Cell Signalling), IDH1 1:1000 (Origene), SP1 1:1000 (Sigma), α-tubulin 1:1000 (Santa Cruz), p-p38 1:1000 (Cell Signalling), p38 1:1000 (Cell Signalling), p-Erk1/2 1:2000 (Cell Signalling), Erk1/2 1:1000 (Cell Signalling), S100A4/FSP1 1:500 (Abnova), TGFBRI 1:1000 (Cell Signalling), TGFBRII 1:1000 (Abcam), p-TGFBRI 1:200 (Abcam), Cav1 1:1000 (ProteinTech), FKBP12 1:1000 (ProteinTech), and SARA 1:1000 (ProteinTech).

Hou X., Zhang J., Wang Y., Xiong W, & Mi J. (2017). TGFBR-IDH1-Cav1 axis promotes TGF-β signalling in cancer-associated fibroblast. Oncotarget, 8(48), 83962-83974.

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