For cell viability assay, 1 × 105 cells were seeded in duplicate in 60-mm dishes. After 4 h for hypoxia followed by 72 h transfection, cells were washed with phosphate buffered saline (PBS) and living cell numbers were determined by trypan blue exclusion (Beyotime Biotechnology, Shanghai, China) as previously described [10 (link)].
Trypan blue exclusion
Manufactured by Beyotime
Sourced in China
Trypan blue exclusion is a laboratory technique used to determine the viability of cells. It is a dye exclusion assay that relies on the principle that live, healthy cells with intact cell membranes do not take up the trypan blue dye, while dead or damaged cells with compromised membranes absorb the dye and appear blue under a microscope.
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3 protocols using trypan blue exclusion
1
Cell Viability Assay Protocol
2
Trypan Blue Viability Assay
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Cell viability was determined by trypan blue exclusion assay. Briefly, 1 × 105 cells were seeded in 60-mm dishes and cultured for 48 h. Then cells were rinsed with phosphate-buffered saline (PBS), and the number of viable cells was determined by trypan blue exclusion (Beyotime Biotechnology, Shanghai, P.R. China) as previously described17 (link).
3
Cell Proliferation Assay Protocol
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For cell proliferation assay, 1 × 10 5 cells were seeded in duplicate in 60-mm dishes. At the indicated time periods, cells were washed phosphatebuffered saline (PBS) and live cell numbers were determined by trypan blue exclusion (Beyotime Biotechnology, Shanghai, China) as previously described [25] .
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