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Anti mouse pd 1 antibody clone rmp1 14

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The Anti-mouse PD-1 antibody (clone RMP1-14) is a laboratory reagent used for research purposes. It is a monoclonal antibody that specifically binds to the mouse programmed cell death protein 1 (PD-1). PD-1 is an important immune checkpoint receptor that plays a role in regulating T-cell activation and function.

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Lab products found in correlation

Erlotinib, by LC Laboratories (1 mentions) Phosphatase inhibitor cocktail, by Merck Group (1 mentions) 1 1 dioctadecyl 3 3 3 3 tetramethylindotricarbocyanine iodide dir, by Merck Group (1 mentions) Tryptone lp0042, by Thermo Fisher Scientific (1 mentions) Yeast extract lp0021, by Thermo Fisher Scientific (1 mentions) Hoechst 33258, by Merck Group (1 mentions) 1 2 dioleoyl sn glycero 3 phosphate dopa, by Avanti Polar Lipids (1 mentions) 2 3 dioleoyloxy propyl trimethylammonium dotap, by Avanti Polar Lipids (1 mentions) Gemcitabine, by Eli Lilly (1 mentions) Anti cd206 pe, by BioLegend (1 mentions) Trustain fcx plus, by BioLegend (1 mentions) Apc anti gr1, by BioLegend (1 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions) Anti cd8, by Cell Signaling Technology (1 mentions) Anti cd3 ab16669, by Abcam (1 mentions) Anti cd4 bv711, by BioLegend (1 mentions) Pe cy7 anti f4 80, by BioLegend (1 mentions) Anti f4 80 70076, by Cell Signaling Technology (1 mentions) Anti cd45 apc cy7, by BioLegend (1 mentions) Anti cd3 pe cy7, by BioLegend (1 mentions)

6 protocols using anti mouse pd 1 antibody clone rmp1 14

1

Erlotinib and anti-PD-1 combination therapy

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Erlotinib (LC Laboratories, Woburn, MA, USA) was solved in 6% Captisol solution and orally administered at a concentration of 50 mg/kg body weight (BW) twice per week. Anti-mouse PD-1 antibody (clone RMP1-14, BioXCell, Lebanon, NH, USA) was administered at 10 mg/kg BW intraperitoneal twice per week [20 (link),21 (link)]. Vehicle mice were treated twice per week with 6% Captisol given orally in combination with intraperitoneal administration of the appropriate murine aPD-1 IgG control (Isotype control rat IgG2a, κ; BioXCell, Lebanon, NH, USA). Mice were randomly assigned to the different therapy groups before start of treatment.
Selenz C., Compes A., Nill M., Borchmann S., Odenthal M., Florin A., Brägelmann J., Büttner R., Meder L, & Ullrich R.T. (2022). EGFR Inhibition Strongly Modulates the Tumour Immune Microenvironment in EGFR-Driven Non-Small-Cell Lung Cancer. Cancers, 14(16), 3943.
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2

Formulation and Characterization of DOTAP/DOPA Lipoplex

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(2,3-dioleoyloxy-propyl)-trimethylammonium (DOTAP) and 1,2-dioleoyl-sn-glycero-3-phosphate (DOPA) were obtained from Avanti Polar Lipids (Alabaster, AL, USA). 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (ammonium salt) (DSPE-PEG2000) was obtained from Xi’an Ruixi biotech (Xi’an, China). FHK peptide (FHKHKSPALSPV) was synthesized by China Peptides Co., Ltd (Shanghai, China). Low molecular weight protamine (LMWP), IGEPAL® CO-520, cholesterol, 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide (DiR), 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT), 4,6-Diamidino-2-phenylindole (DAPI), Hoechst 33258 and phosphatase inhibitor cocktails were provided by Sigma-Aldrich (St. Louis, MO, USA). α-M were purchased from Biopurify Co., Ltd (Chengdu, China). Collagenase type IV (40510ES60), hyaluronidase (20426ES60), DNase I (10608ES25) and Agar (70101ES76) were purchased from Shanghai yeasen biotech (Shanghai, China). Tryptone (LP0042) and Yeast extract (LP0021) were obtained from OXOID. Gemcitabine was obtained from Eli Lilly. Anti-mouse PD-1 antibody (clone: RMP1-14) and IgG isotype (MPC-11, BP0086) are acquired from BioXcell. All the other chemical reagents and solvents were acquired from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China) unless specified.
Huang Y., Chen Y., Zhou S., Chen L., Wang J., Pei Y., Xu M., Feng J., Jiang T., Liang K., Liu S., Song Q., Jiang G., Gu X., Zhang Q., Gao X, & Chen J. (2020). Dual-mechanism based CTLs infiltration enhancement initiated by Nano-sapper potentiates immunotherapy against immune-excluded tumors. Nature Communications, 11, 622.
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3

Antibody-Drug Conjugate Preclinical Evaluation

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RC48 and Disitamab were provided by RemeGen Co., Ltd. Monomethyl auristatin E (MMAE, 474645-27-7) was from Topscience Co. Ltd. Irinotecan (CPT-11, I1406), were purchased from Sigma-Aldrich LLC. Anti-mouse PD-1 antibody (clone RMP1-14) was purchased from Bio X Cell.
Wu X., Xu L., Li X., Zhou Y., Han X., Zhang W., Wang W., Guo W., Liu W., Xu Q, & Gu Y. (2023). A HER2-targeting antibody-MMAE conjugate RC48 sensitizes immunotherapy in HER2-positive colon cancer by triggering the cGAS-STING pathway. Cell Death & Disease, 14(8), 550.
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4

WT1 Peptide Vaccine and Anti-PD-1 Therapy

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For WT1 peptide vaccine therapy, both WT1126–134 (RMFPNAPYL) and WT135–52 (WAPVLDFAPPGASAYGSL) peptides (SIGMA Genosys) were prepared as oil-in-water emulsion with Montanide ISA 51 (Seppic S.A.) and subcutaneously and weekly injected for a total of five times on axillary regions. Amino acid sequences of these two mouse WT1 peptides were the same as those of human WT1 peptides. For anti-PD-1 antibody therapy, 200 μg of anti-mouse PD-1 antibody (clone RMP1-14, BioXCell) was intraperitoneally injected twice a week for a total of nine times. In the combination therapy, WT1 peptide vaccine and anti-PD-1 antibody therapies were simultaneously performed.
Yokota C., Nakata J., Takano K., Nakajima H., Hayashibara H., Minagawa H., Chiba Y., Hirayama R., Kijima N., Kinoshita M., Hashii Y., Tsuboi A., Oka Y., Oji Y., Kumanogoh A., Sugiyama H., Kagawa N, & Kishima H. (2021). Distinct difference in tumor-infiltrating immune cells between Wilms’ tumor gene 1 peptide vaccine and anti-programmed cell death-1 antibody therapies. Neuro-oncology Advances, 3(1), vdab091.
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5

Anti-PD-1 Antibody Treatment for Tumor Regression

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Anti-mouse PD-1 antibody (clone RMP1-14) was purchased from BioXcell (West Lebanon, NH, USA). The antibody (250 μg) in 200 μL PBS was administered twice intraperitoneally to the tumor-bearing mice on Day 0 and Day 5. Mice with anti-PD-1 antibody were defined as the treatment group. In addition to the treatment group, the control group was prepared, which consisted of mice receiving 200 μL PBS without anti-PD-1 antibody twice intraperitoneally on Day 0 and Day 5.
Tumors were measured using calipers, and the tumor volume expressed in mm3 was calculated according to the following formula: 0.5 × (long diameter) × (short diameter)2. Relative tumor volume, the volume ratio of posttreatment to pretreatment tumor, was also calculated in this study.
Tatsumi M., Soeda F., Naka S., Kurimoto K., Ooe K., Fukui H., Katayama D., Watabe T., Kato H, & Tomiyama N. (2022). Advantages of FBPA PET in evaluating early response of anti-PD-1 immunotherapy in B16F10 melanoma-bearing mice: Comparison to FDG PET. Frontiers in Oncology, 12, 1026608.
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6

Immune Cell Profiling in Tumor Models

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Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were obtained from Gibco (Grand Island, NY, USA). Matrigel was purchased from Corning, Inc. (Corning, NY, USA). Anti-mouse PD-1 antibody (clone RMP1-14) and isotype control IgG2a antibody were obtained from BioXCell (West Lebanon, NH, USA). Anti-CD3 (#ab16669) and anti-TGF-β1 (#ab215715) antibodies were purchased from Abcam (Cambridge, MA, USA). Anti-CD8 (#98941) and anti-F4/80 (#70076) antibodies were purchased from Cell Signaling (Beverly, MA, USA). Anti-granzyme B antibody (#14-8822-82) was purchased from Invitrogen (Carlsbad, CA, USA). PE/Cy7 anti-CD3 (#552774), BV605 Anti-CD8 (#563152), BV711 anti-CD4 (#563050), APC anti-CD335 (NKp46) (#137608), APC/Cy7 anti-CD45 (#103116), BV421 anti-CD11b (#562605), APC anti-GR-1 (#108412), PE/Cy7 anti-F4/80 (#123114), Alexa488 anti-MHC Class II (#107616), PE anti-CD206 (#141706), and TruStain FcX™ PLUS (#156604) antibodies were purchased from Biolegend (San Diego, CA, USA).
Chun J., Park S.M., Lee M., Ha I.J, & Jeong M.K. (2023). The Sesquiterpene Lactone-Rich Fraction of Inula helenium L. Enhances the Antitumor Effect of Anti-PD-1 Antibody in Colorectal Cancer: Integrative Phytochemical, Transcriptomic, and Experimental Analyses. Cancers, 15(3), 653.
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