Tissue culture 6 well plates

Tissue culture 6-well plates (Falcon) were used for all adsorption and absorption experiments. All cells were seeded at a density of 1 × 10⁶ per well and allowed to grow to confluence (3 to 5 days). Phages Slur96 and T7 were applied with DMEM medium and incubated with cells for either one or four hours. The medium (pre-wash) was removed, followed by the addition of PBS while gently pipetting up and down. The PBS addition was repeated twice, and the resulting medium (wash step) was collected. Then, 0.5% saponin (Sigma Aldrich, St. Louis, MO, USA) was added to each well, and the plate was incubated for 15 min at 37 °C. After the incubation, the saponin in each well was pipetted up and down, followed by sample collection (lysis step). The samples collected during the pre-wash, wash, and lysis steps were used for phage quantification by plating with their bacterial host.

Nucleated bone marrow cells were plated into tissue culture 6-well plates (BD Falcon) at a density of <10⁵ cells/cm² and cultured in low-glucose DMEM with GlutaMAX supplement (Gibco 10567022) and 10% mesenchymal stem cell-qualified FBS (Gibco 12662029) containing penicillin-streptomycin (Sigma P0781) for 10∼14 days. Cell cultures were maintained at 37 ^°C in a 5% CO₂ incubator. Representative images of at least three independent biological samples are shown in the figures. For CFU-Fs, cells were fixed with 70% Ethanol for 5 min and stained for 2% methylene blue.

Nucleated bone marrow cells were plated into tissue culture 6-well plates (BD Falcon) at a density of <10⁵ cells/cm², and cultured in low-glucose DMEM with GlutaMAX supplement (Gibco 10567022) and 10% mesenchymal stem cell-qualified FBS (Gibco 12662029) containing penicillin–streptomycin (Sigma P0781) for 10–14 days. Cell cultures were maintained at 37 °C in a 5% CO₂ incubator. Representative images of at least three independent biological samples are shown in the figures.